Genotoxicity of Silver Nanoparticles in Lung Cells of Sprague Dawley Rats after 12 Weeks of Inhalation Exposure
Due to the widespread use of silver nanoparticles in consumer products, the toxicity of silver nanoparticles has also been studied in relation to their application. However, most genotoxicity studies of silver nanoparticles have been performed in vitro. Therefore, this study evaluated the DNA damage...
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| Format: | Article |
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MDPI AG
2013-11-01
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| Series: | Toxics |
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| Online Access: | http://www.mdpi.com/2305-6304/1/1/36 |
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| author | Hyun Sun Cho Jae Hyuck Sung Kyung Seuk Song Jin Sik Kim Jun Ho Ji Ji Hyun Lee Hyeon Ryol Ryu Kangho Ahn Il Je Yu |
| author_facet | Hyun Sun Cho Jae Hyuck Sung Kyung Seuk Song Jin Sik Kim Jun Ho Ji Ji Hyun Lee Hyeon Ryol Ryu Kangho Ahn Il Je Yu |
| author_sort | Hyun Sun Cho |
| collection | DOAJ |
| description | Due to the widespread use of silver nanoparticles in consumer products, the toxicity of silver nanoparticles has also been studied in relation to their application. However, most genotoxicity studies of silver nanoparticles have been performed in vitro. Therefore, this study evaluated the DNA damage to lung cells caused by repeated inhalation of silver nanoparticles. Male Sprague Dawley rats were exposed to silver nanoparticles for 12 weeks in a whole-body inhalation chamber. The animals were divided into one control group and three dose groups that were exposed to silver nanoparticles (14–15 nm diameter) at concentrations of 0.66 × 106 particles/cm3 (49 μg/m3, low dose), 1.41 × 106 particles/cm3 (117 μg/m3, middle dose), and 3.24 × 106 particles /cm3 (381 μg/m3, high dose), respectively, for six hours/day over 12 weeks. The rats were sacrificed after the 12-week exposure period and the DNA damage assessed using a Comet assay of cells obtained from the right lungs. The olive tail moment values were 2.93 ± 0.19, 3.81 ± 0.23, 3.40 ± 0.22, and 5.16 ± 0.32 for the control, low-, middle-, and high-dose groups, respectively. Although no dose-dependent results were observed, a significant increase in the level of DNA damage was noted for the high-dose group. |
| first_indexed | 2024-12-20T05:24:53Z |
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| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-12-20T05:24:53Z |
| publishDate | 2013-11-01 |
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| series | Toxics |
| spelling | doaj.art-4c1b2d8e4b0c4ff49715b1807274491d2022-12-21T19:51:55ZengMDPI AGToxics2305-63042013-11-0111364510.3390/toxics1010036toxics1010036Genotoxicity of Silver Nanoparticles in Lung Cells of Sprague Dawley Rats after 12 Weeks of Inhalation ExposureHyun Sun Cho0Jae Hyuck Sung1Kyung Seuk Song2Jin Sik Kim3Jun Ho Ji4Ji Hyun Lee5Hyeon Ryol Ryu6Kangho Ahn7Il Je Yu8Bioconvergence Department, Korea Conformity Laboratories, Incheon 406-840, KoreaBioconvergence Department, Korea Conformity Laboratories, Incheon 406-840, KoreaBioconvergence Department, Korea Conformity Laboratories, Incheon 406-840, KoreaBioconvergence Department, Korea Conformity Laboratories, Incheon 406-840, KoreaCenter for Environment, Health and Welfare Research, Korea Institute of Science and Technology, Seoul 136-791, KoreaInstitute of Nanoproduct Safety Research, Hoseo University, 165 Sechul-ri, Baebang-myun, Asan 336-795, KoreaBioconvergence Department, Korea Conformity Laboratories, Incheon 406-840, KoreaDepartment of Mechanical Engineering, Hanyang University, Seoul 133-791, KoreaInstitute of Nanoproduct Safety Research, Hoseo University, 165 Sechul-ri, Baebang-myun, Asan 336-795, KoreaDue to the widespread use of silver nanoparticles in consumer products, the toxicity of silver nanoparticles has also been studied in relation to their application. However, most genotoxicity studies of silver nanoparticles have been performed in vitro. Therefore, this study evaluated the DNA damage to lung cells caused by repeated inhalation of silver nanoparticles. Male Sprague Dawley rats were exposed to silver nanoparticles for 12 weeks in a whole-body inhalation chamber. The animals were divided into one control group and three dose groups that were exposed to silver nanoparticles (14–15 nm diameter) at concentrations of 0.66 × 106 particles/cm3 (49 μg/m3, low dose), 1.41 × 106 particles/cm3 (117 μg/m3, middle dose), and 3.24 × 106 particles /cm3 (381 μg/m3, high dose), respectively, for six hours/day over 12 weeks. The rats were sacrificed after the 12-week exposure period and the DNA damage assessed using a Comet assay of cells obtained from the right lungs. The olive tail moment values were 2.93 ± 0.19, 3.81 ± 0.23, 3.40 ± 0.22, and 5.16 ± 0.32 for the control, low-, middle-, and high-dose groups, respectively. Although no dose-dependent results were observed, a significant increase in the level of DNA damage was noted for the high-dose group.http://www.mdpi.com/2305-6304/1/1/36silver nanoparticlelung cellsgenotoxicitycomet assaysingle cell gel electrophoresisinhalation exposure |
| spellingShingle | Hyun Sun Cho Jae Hyuck Sung Kyung Seuk Song Jin Sik Kim Jun Ho Ji Ji Hyun Lee Hyeon Ryol Ryu Kangho Ahn Il Je Yu Genotoxicity of Silver Nanoparticles in Lung Cells of Sprague Dawley Rats after 12 Weeks of Inhalation Exposure Toxics silver nanoparticle lung cells genotoxicity comet assay single cell gel electrophoresis inhalation exposure |
| title | Genotoxicity of Silver Nanoparticles in Lung Cells of Sprague Dawley Rats after 12 Weeks of Inhalation Exposure |
| title_full | Genotoxicity of Silver Nanoparticles in Lung Cells of Sprague Dawley Rats after 12 Weeks of Inhalation Exposure |
| title_fullStr | Genotoxicity of Silver Nanoparticles in Lung Cells of Sprague Dawley Rats after 12 Weeks of Inhalation Exposure |
| title_full_unstemmed | Genotoxicity of Silver Nanoparticles in Lung Cells of Sprague Dawley Rats after 12 Weeks of Inhalation Exposure |
| title_short | Genotoxicity of Silver Nanoparticles in Lung Cells of Sprague Dawley Rats after 12 Weeks of Inhalation Exposure |
| title_sort | genotoxicity of silver nanoparticles in lung cells of sprague dawley rats after 12 weeks of inhalation exposure |
| topic | silver nanoparticle lung cells genotoxicity comet assay single cell gel electrophoresis inhalation exposure |
| url | http://www.mdpi.com/2305-6304/1/1/36 |
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