Tracking B Cell Memory to SARS-CoV-2 Using Rare Cell Analysis System
Rapid mutations within SARS-CoV-2 are driving immune escape, highlighting the need for in-depth and routine analysis of memory B cells (MBCs) to complement the important but limited information from neutralizing antibody (nAb) studies. In this study, we collected plasma samples and peripheral blood...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Vaccines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-393X/11/4/735 |
| _version_ | 1797603281542840320 |
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| author | Dong-Yan Tsai Chun-Hung Wang Perry G. Schiro Nathan Chen Ju-Yu Tseng |
| author_facet | Dong-Yan Tsai Chun-Hung Wang Perry G. Schiro Nathan Chen Ju-Yu Tseng |
| author_sort | Dong-Yan Tsai |
| collection | DOAJ |
| description | Rapid mutations within SARS-CoV-2 are driving immune escape, highlighting the need for in-depth and routine analysis of memory B cells (MBCs) to complement the important but limited information from neutralizing antibody (nAb) studies. In this study, we collected plasma samples and peripheral blood mononuclear cells (PBMCs) from 35 subjects and studied the nAb titers and the number of antigen-specific memory B cells at designated time points before and after vaccination. We developed an assay to use the MiSelect R II System with a single-use microfluidic chip to directly detect the number of spike-receptor-binding domain (RBD)-specific MBCs in PBMCs. Our results show that the number of spike-RBD-specific MBCs detected by the MiSelect R II System is highly correlated with the level of nAbs secreted by stimulated PBMCs, even 6 months after vaccination when nAbs were generally not present in plasma. We also found antigen-specific cells recognizing Omicron spike-RBD were present in PBMCs from booster vaccination of subjects, but with a high variability in the number of B cells. The MiSelect R II System provided a direct, automated, and quantitative method to isolate and analyze subsets of rare cells for tracking cellular immunity in the context of a rapidly mutating virus. |
| first_indexed | 2024-03-11T04:27:58Z |
| format | Article |
| id | doaj.art-4c1be2019fa44063bc6fa601c49928a8 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-11T04:27:58Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-4c1be2019fa44063bc6fa601c49928a82023-11-17T21:41:00ZengMDPI AGVaccines2076-393X2023-03-0111473510.3390/vaccines11040735Tracking B Cell Memory to SARS-CoV-2 Using Rare Cell Analysis SystemDong-Yan Tsai0Chun-Hung Wang1Perry G. Schiro2Nathan Chen3Ju-Yu Tseng4MiCareo Taiwan Co., Ltd., 5F, No. 69, Ln. 77, Xing Ai Rd., Neihu Dist., Taipei City 114, TaiwanMiCareo Taiwan Co., Ltd., 5F, No. 69, Ln. 77, Xing Ai Rd., Neihu Dist., Taipei City 114, TaiwanMiCareo Taiwan Co., Ltd., 5F, No. 69, Ln. 77, Xing Ai Rd., Neihu Dist., Taipei City 114, TaiwanAdimmune Corporation, No. 3, Sec.1, Tanxing Rd., Tanzi Dist., Taichung City 427, TaiwanMiCareo Taiwan Co., Ltd., 5F, No. 69, Ln. 77, Xing Ai Rd., Neihu Dist., Taipei City 114, TaiwanRapid mutations within SARS-CoV-2 are driving immune escape, highlighting the need for in-depth and routine analysis of memory B cells (MBCs) to complement the important but limited information from neutralizing antibody (nAb) studies. In this study, we collected plasma samples and peripheral blood mononuclear cells (PBMCs) from 35 subjects and studied the nAb titers and the number of antigen-specific memory B cells at designated time points before and after vaccination. We developed an assay to use the MiSelect R II System with a single-use microfluidic chip to directly detect the number of spike-receptor-binding domain (RBD)-specific MBCs in PBMCs. Our results show that the number of spike-RBD-specific MBCs detected by the MiSelect R II System is highly correlated with the level of nAbs secreted by stimulated PBMCs, even 6 months after vaccination when nAbs were generally not present in plasma. We also found antigen-specific cells recognizing Omicron spike-RBD were present in PBMCs from booster vaccination of subjects, but with a high variability in the number of B cells. The MiSelect R II System provided a direct, automated, and quantitative method to isolate and analyze subsets of rare cells for tracking cellular immunity in the context of a rapidly mutating virus.https://www.mdpi.com/2076-393X/11/4/735SARS-CoV-2vaccinesrare cellsmemory B cells |
| spellingShingle | Dong-Yan Tsai Chun-Hung Wang Perry G. Schiro Nathan Chen Ju-Yu Tseng Tracking B Cell Memory to SARS-CoV-2 Using Rare Cell Analysis System Vaccines SARS-CoV-2 vaccines rare cells memory B cells |
| title | Tracking B Cell Memory to SARS-CoV-2 Using Rare Cell Analysis System |
| title_full | Tracking B Cell Memory to SARS-CoV-2 Using Rare Cell Analysis System |
| title_fullStr | Tracking B Cell Memory to SARS-CoV-2 Using Rare Cell Analysis System |
| title_full_unstemmed | Tracking B Cell Memory to SARS-CoV-2 Using Rare Cell Analysis System |
| title_short | Tracking B Cell Memory to SARS-CoV-2 Using Rare Cell Analysis System |
| title_sort | tracking b cell memory to sars cov 2 using rare cell analysis system |
| topic | SARS-CoV-2 vaccines rare cells memory B cells |
| url | https://www.mdpi.com/2076-393X/11/4/735 |
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