Genomic characterization of an Omono River virus isolated from Culex tritaeniorhynchus in eastern China
Abstract Omono River virus (OMRV) is a newly reported, unclassified RNA virus in the family Totiviridae, which infects mosquitoes and bats. In this study, we report the isolation of an OMRV strain SD76 from Culex tritaeniorhynchus captured in Jinan city, China. The cytopathic effect was characterize...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-04-01
|
| Series: | Virology Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12985-023-02041-y |
| _version_ | 1827961474085027840 |
|---|---|
| author | Xiaojuan Lin Bo Sun Guifang Liu Yunjiao Wu Yao Liu Feng Ji Zexin Tao Aiqiang Xu |
| author_facet | Xiaojuan Lin Bo Sun Guifang Liu Yunjiao Wu Yao Liu Feng Ji Zexin Tao Aiqiang Xu |
| author_sort | Xiaojuan Lin |
| collection | DOAJ |
| description | Abstract Omono River virus (OMRV) is a newly reported, unclassified RNA virus in the family Totiviridae, which infects mosquitoes and bats. In this study, we report the isolation of an OMRV strain SD76 from Culex tritaeniorhynchus captured in Jinan city, China. The cytopathic effect was characterized by cell fusion on C6/36 cell line. Its complete genome was 7611 nucleotides in length, with 71.4–90.4% similarities with other OMRV strains. Phylogenetic analysis based on complete genomes showed all OMRV-like strains can be divided into 3 groups with between-group distances ranging from 0.254 to 0.293. These results revealed that the OMRV isolate had high genetic diversity with those identified previously, and enriched the genetic information of family Totiviridae. |
| first_indexed | 2024-04-09T16:26:14Z |
| format | Article |
| id | doaj.art-4c1c54ac0ef14dc0b63d27c01f585c5c |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-04-09T16:26:14Z |
| publishDate | 2023-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-4c1c54ac0ef14dc0b63d27c01f585c5c2023-04-23T11:09:26ZengBMCVirology Journal1743-422X2023-04-012011510.1186/s12985-023-02041-yGenomic characterization of an Omono River virus isolated from Culex tritaeniorhynchus in eastern ChinaXiaojuan Lin0Bo Sun1Guifang Liu2Yunjiao Wu3Yao Liu4Feng Ji5Zexin Tao6Aiqiang Xu7Shandong Center for Disease Control and PreventionTianqiao Center for Disease Control and PreventionShandong Center for Disease Control and PreventionSchool of Public Health, Shandong First Medical University & Shandong Academy of Medical SciencesShandong Center for Disease Control and PreventionShandong Center for Disease Control and PreventionShandong Center for Disease Control and PreventionShandong Center for Disease Control and PreventionAbstract Omono River virus (OMRV) is a newly reported, unclassified RNA virus in the family Totiviridae, which infects mosquitoes and bats. In this study, we report the isolation of an OMRV strain SD76 from Culex tritaeniorhynchus captured in Jinan city, China. The cytopathic effect was characterized by cell fusion on C6/36 cell line. Its complete genome was 7611 nucleotides in length, with 71.4–90.4% similarities with other OMRV strains. Phylogenetic analysis based on complete genomes showed all OMRV-like strains can be divided into 3 groups with between-group distances ranging from 0.254 to 0.293. These results revealed that the OMRV isolate had high genetic diversity with those identified previously, and enriched the genetic information of family Totiviridae.https://doi.org/10.1186/s12985-023-02041-yOmono River virusMosquitoComplete genomePhylogeny |
| spellingShingle | Xiaojuan Lin Bo Sun Guifang Liu Yunjiao Wu Yao Liu Feng Ji Zexin Tao Aiqiang Xu Genomic characterization of an Omono River virus isolated from Culex tritaeniorhynchus in eastern China Virology Journal Omono River virus Mosquito Complete genome Phylogeny |
| title | Genomic characterization of an Omono River virus isolated from Culex tritaeniorhynchus in eastern China |
| title_full | Genomic characterization of an Omono River virus isolated from Culex tritaeniorhynchus in eastern China |
| title_fullStr | Genomic characterization of an Omono River virus isolated from Culex tritaeniorhynchus in eastern China |
| title_full_unstemmed | Genomic characterization of an Omono River virus isolated from Culex tritaeniorhynchus in eastern China |
| title_short | Genomic characterization of an Omono River virus isolated from Culex tritaeniorhynchus in eastern China |
| title_sort | genomic characterization of an omono river virus isolated from culex tritaeniorhynchus in eastern china |
| topic | Omono River virus Mosquito Complete genome Phylogeny |
| url | https://doi.org/10.1186/s12985-023-02041-y |
| work_keys_str_mv | AT xiaojuanlin genomiccharacterizationofanomonorivervirusisolatedfromculextritaeniorhynchusineasternchina AT bosun genomiccharacterizationofanomonorivervirusisolatedfromculextritaeniorhynchusineasternchina AT guifangliu genomiccharacterizationofanomonorivervirusisolatedfromculextritaeniorhynchusineasternchina AT yunjiaowu genomiccharacterizationofanomonorivervirusisolatedfromculextritaeniorhynchusineasternchina AT yaoliu genomiccharacterizationofanomonorivervirusisolatedfromculextritaeniorhynchusineasternchina AT fengji genomiccharacterizationofanomonorivervirusisolatedfromculextritaeniorhynchusineasternchina AT zexintao genomiccharacterizationofanomonorivervirusisolatedfromculextritaeniorhynchusineasternchina AT aiqiangxu genomiccharacterizationofanomonorivervirusisolatedfromculextritaeniorhynchusineasternchina |