Effect of standard and neutral-pH peritoneal dialysis solutions upon fibroblasts proliferation
Introduction: Continuous exposition of the peritoneal membrane to conventional dialysis solutions is an important risk factor for inducing structural and functional alterations. Objective: To compare in vitro mouse fibroblast NIH-3T3 cell viability after exposition to a neutral pH dialysis solutio...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Nefrologia
2014-04-01
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| Series: | Brazilian Journal of Nephrology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-28002014000200150&lng=en&tlng=en |
| _version_ | 1819081794703589376 |
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| author | André Antunes Poitevin Christian Viezzer Denise Cantarelli Machado Bartira Ercilia Pinheiro da Costa Ana Elizabeth Figueiredo Domingos d'Avila Carlos Eduardo Poli-de-Figueiredo |
| author_facet | André Antunes Poitevin Christian Viezzer Denise Cantarelli Machado Bartira Ercilia Pinheiro da Costa Ana Elizabeth Figueiredo Domingos d'Avila Carlos Eduardo Poli-de-Figueiredo |
| author_sort | André Antunes Poitevin |
| collection | DOAJ |
| description | Introduction: Continuous exposition of the peritoneal membrane to conventional dialysis solutions is an important risk factor for inducing structural and functional alterations. Objective: To compare in vitro mouse fibroblast NIH-3T3 cell viability after exposition to a neutral pH dialysis solution in comparison to cells exposed to a standard solution. Methods: Experimental study to compare the effects of a conventional standard or a neutral-pH, low-glucose degradation products peritoneal dialysis solution on the viability of exposed fibroblasts in cell culture. Both solutions were tested in all the commercially available glucose concentrations. Cell viability was evaluated with tetrazolium salt colorimetric assay. Results: Fibroblast viability was significantly superior in the neutral pH solution in comparison to control, in all three glucose concentrations (Optical density in nm-means ± SD: 1.5% 0.295 ± 0.047 vs. 0.372 ± 0.042, p < 0.001; 2.3% 0.270 ± 0.036 vs. 0.337 ± 0.051, p < 0.001; 4.25% 0.284 ± 0.037 vs. 0.332 ± 0.032, p < 0.001; control vs. neutral pH respectively, Student t Test). There was no significant difference in cell viability between the three concentrations of glucose when standard solution was used (ANOVA p = 0.218), although cell viability was higher after exposition to neutral pH peritoneal dialysis fluid at 1.5% in comparison to 2.3 and 4.25% glucose concentrations (ANOVA p = 0.008: Bonferroni 1.5% vs. 2.3% p = 0.033, 1.5% vs. 4.25% p = 0.014, 2.3% vs. 4.25% p = 1.00). Conclusion: Cell viability was better in neutral pH dialysis solution, especially in the lower glucose concentration. A more physiological pH and lower glucose degradation products may be responsible for such results. |
| first_indexed | 2024-12-21T20:06:27Z |
| format | Article |
| id | doaj.art-4c1cc010143a4820b7e6c48b252f5f15 |
| institution | Directory Open Access Journal |
| issn | 2175-8239 |
| language | English |
| last_indexed | 2024-12-21T20:06:27Z |
| publishDate | 2014-04-01 |
| publisher | Sociedade Brasileira de Nefrologia |
| record_format | Article |
| series | Brazilian Journal of Nephrology |
| spelling | doaj.art-4c1cc010143a4820b7e6c48b252f5f152022-12-21T18:51:49ZengSociedade Brasileira de NefrologiaBrazilian Journal of Nephrology2175-82392014-04-0136215015410.5935/0101-2800.20140024S0101-28002014000200150Effect of standard and neutral-pH peritoneal dialysis solutions upon fibroblasts proliferationAndré Antunes PoitevinChristian ViezzerDenise Cantarelli MachadoBartira Ercilia Pinheiro da CostaAna Elizabeth FigueiredoDomingos d'AvilaCarlos Eduardo Poli-de-FigueiredoIntroduction: Continuous exposition of the peritoneal membrane to conventional dialysis solutions is an important risk factor for inducing structural and functional alterations. Objective: To compare in vitro mouse fibroblast NIH-3T3 cell viability after exposition to a neutral pH dialysis solution in comparison to cells exposed to a standard solution. Methods: Experimental study to compare the effects of a conventional standard or a neutral-pH, low-glucose degradation products peritoneal dialysis solution on the viability of exposed fibroblasts in cell culture. Both solutions were tested in all the commercially available glucose concentrations. Cell viability was evaluated with tetrazolium salt colorimetric assay. Results: Fibroblast viability was significantly superior in the neutral pH solution in comparison to control, in all three glucose concentrations (Optical density in nm-means ± SD: 1.5% 0.295 ± 0.047 vs. 0.372 ± 0.042, p < 0.001; 2.3% 0.270 ± 0.036 vs. 0.337 ± 0.051, p < 0.001; 4.25% 0.284 ± 0.037 vs. 0.332 ± 0.032, p < 0.001; control vs. neutral pH respectively, Student t Test). There was no significant difference in cell viability between the three concentrations of glucose when standard solution was used (ANOVA p = 0.218), although cell viability was higher after exposition to neutral pH peritoneal dialysis fluid at 1.5% in comparison to 2.3 and 4.25% glucose concentrations (ANOVA p = 0.008: Bonferroni 1.5% vs. 2.3% p = 0.033, 1.5% vs. 4.25% p = 0.014, 2.3% vs. 4.25% p = 1.00). Conclusion: Cell viability was better in neutral pH dialysis solution, especially in the lower glucose concentration. A more physiological pH and lower glucose degradation products may be responsible for such results.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-28002014000200150&lng=en&tlng=endiálise peritonealfibroblastosteste de materiaistécnicas de cultura de células |
| spellingShingle | André Antunes Poitevin Christian Viezzer Denise Cantarelli Machado Bartira Ercilia Pinheiro da Costa Ana Elizabeth Figueiredo Domingos d'Avila Carlos Eduardo Poli-de-Figueiredo Effect of standard and neutral-pH peritoneal dialysis solutions upon fibroblasts proliferation Brazilian Journal of Nephrology diálise peritoneal fibroblastos teste de materiais técnicas de cultura de células |
| title | Effect of standard and neutral-pH peritoneal dialysis solutions upon fibroblasts proliferation |
| title_full | Effect of standard and neutral-pH peritoneal dialysis solutions upon fibroblasts proliferation |
| title_fullStr | Effect of standard and neutral-pH peritoneal dialysis solutions upon fibroblasts proliferation |
| title_full_unstemmed | Effect of standard and neutral-pH peritoneal dialysis solutions upon fibroblasts proliferation |
| title_short | Effect of standard and neutral-pH peritoneal dialysis solutions upon fibroblasts proliferation |
| title_sort | effect of standard and neutral ph peritoneal dialysis solutions upon fibroblasts proliferation |
| topic | diálise peritoneal fibroblastos teste de materiais técnicas de cultura de células |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-28002014000200150&lng=en&tlng=en |
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