S-Adenosyl-L-Methionine and Cu(II) Impact Green Plant Regeneration Efficiency
The biological improvement of triticale, a cereal of increasing importance in agriculture, may be accelerated via the production of doubled haploid lines using in vitro culture. Among the relevant factors affecting the culture efficiency are Cu(II) or Ag(I) acting, e.g., as cofactors of enzymes. The...
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MDPI AG
2022-08-01
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| author | Renata Orłowska Jacek Zebrowski Janusz Zimny Piotr Androsiuk Piotr Tomasz Bednarek |
| author_facet | Renata Orłowska Jacek Zebrowski Janusz Zimny Piotr Androsiuk Piotr Tomasz Bednarek |
| author_sort | Renata Orłowska |
| collection | DOAJ |
| description | The biological improvement of triticale, a cereal of increasing importance in agriculture, may be accelerated via the production of doubled haploid lines using in vitro culture. Among the relevant factors affecting the culture efficiency are Cu(II) or Ag(I) acting, e.g., as cofactors of enzymes. The copper ions are known to positively affect green plant regeneration efficiency. However, the biochemical basis, mainly its role in the generation of in vitro-induced genetic and epigenetic variation and green plant regeneration efficiency, is not well understood. Here, we employed structural equation modeling to evaluate the relationship between de novo DNA methylation affecting the asymmetric context of CHH sequences, the methylation-sensitive Amplified Fragment Length Polymorphism related sequence variation, and the concentration of Cu(II) and Ag(I) ions in induction media, as well as their effect on S-adenosyl-L-methionine perturbations, observed using FTIR spectroscopy, and the green plant regeneration efficiency. Our results allowed the construction of a theory-based model reflecting the biological phenomena associated with green plant regeneration efficiency. Furthermore, it is shown that Cu(II) ions in induction media affect plant regeneration, and by manipulating their concentration, the regeneration efficiency can be altered. Additionally, S-adenosyl-L-methionine is involved in the efficiency of green plant regeneration through methylation of the asymmetric CHH sequence related to de novo methylation. This shows that the Yang cycle may impact the production of green regenerants. |
| first_indexed | 2024-03-10T01:56:45Z |
| format | Article |
| id | doaj.art-4c1f04d339284ee3ba6c2d77e179c9ba |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T01:56:45Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-4c1f04d339284ee3ba6c2d77e179c9ba2023-11-23T12:55:27ZengMDPI AGCells2073-44092022-08-011117270010.3390/cells11172700S-Adenosyl-L-Methionine and Cu(II) Impact Green Plant Regeneration EfficiencyRenata Orłowska0Jacek Zebrowski1Janusz Zimny2Piotr Androsiuk3Piotr Tomasz Bednarek4Plant Breeding and Acclimatization Institute—National Research Institute, Radzików, 05-870 Błonie, PolandInstitute of Biology and Biotechnology, University of Rzeszow, Pigonia 1, 35-310 Rzeszow, PolandPlant Breeding and Acclimatization Institute—National Research Institute, Radzików, 05-870 Błonie, PolandDepartment of Plant Physiology, Genetics and Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, PolandPlant Breeding and Acclimatization Institute—National Research Institute, Radzików, 05-870 Błonie, PolandThe biological improvement of triticale, a cereal of increasing importance in agriculture, may be accelerated via the production of doubled haploid lines using in vitro culture. Among the relevant factors affecting the culture efficiency are Cu(II) or Ag(I) acting, e.g., as cofactors of enzymes. The copper ions are known to positively affect green plant regeneration efficiency. However, the biochemical basis, mainly its role in the generation of in vitro-induced genetic and epigenetic variation and green plant regeneration efficiency, is not well understood. Here, we employed structural equation modeling to evaluate the relationship between de novo DNA methylation affecting the asymmetric context of CHH sequences, the methylation-sensitive Amplified Fragment Length Polymorphism related sequence variation, and the concentration of Cu(II) and Ag(I) ions in induction media, as well as their effect on S-adenosyl-L-methionine perturbations, observed using FTIR spectroscopy, and the green plant regeneration efficiency. Our results allowed the construction of a theory-based model reflecting the biological phenomena associated with green plant regeneration efficiency. Furthermore, it is shown that Cu(II) ions in induction media affect plant regeneration, and by manipulating their concentration, the regeneration efficiency can be altered. Additionally, S-adenosyl-L-methionine is involved in the efficiency of green plant regeneration through methylation of the asymmetric CHH sequence related to de novo methylation. This shows that the Yang cycle may impact the production of green regenerants.https://www.mdpi.com/2073-4409/11/17/2700anther culturecoppergenetic variationmetAFLPS-adenosyl-L-methioninesilver |
| spellingShingle | Renata Orłowska Jacek Zebrowski Janusz Zimny Piotr Androsiuk Piotr Tomasz Bednarek S-Adenosyl-L-Methionine and Cu(II) Impact Green Plant Regeneration Efficiency Cells anther culture copper genetic variation metAFLP S-adenosyl-L-methionine silver |
| title | S-Adenosyl-L-Methionine and Cu(II) Impact Green Plant Regeneration Efficiency |
| title_full | S-Adenosyl-L-Methionine and Cu(II) Impact Green Plant Regeneration Efficiency |
| title_fullStr | S-Adenosyl-L-Methionine and Cu(II) Impact Green Plant Regeneration Efficiency |
| title_full_unstemmed | S-Adenosyl-L-Methionine and Cu(II) Impact Green Plant Regeneration Efficiency |
| title_short | S-Adenosyl-L-Methionine and Cu(II) Impact Green Plant Regeneration Efficiency |
| title_sort | s adenosyl l methionine and cu ii impact green plant regeneration efficiency |
| topic | anther culture copper genetic variation metAFLP S-adenosyl-L-methionine silver |
| url | https://www.mdpi.com/2073-4409/11/17/2700 |
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