Alteration of the gut microbiome and correlated metabolism in a rat model of long-term depression
ObjectiveThis study aims to investigate the composition and function of the gut microbiome in long-term depression using an 8-week chronic unpredictable mild stress (CUMS) rat model.Materials and methodsAnimals were sacrificed after either 4 weeks or 8 weeks under CUMS to mimic long-term depression...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1116277/full |
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| author | Yubo Li Junling Li Ran Cheng Haixia Liu Yukun Zhao Yanjun Liu Yanjing Chen Zhibo Sun Zhiguang Zhai Meng Wu Yupeng Yan Yuxiu Sun Zhiguo Zhang |
| author_facet | Yubo Li Junling Li Ran Cheng Haixia Liu Yukun Zhao Yanjun Liu Yanjing Chen Zhibo Sun Zhiguang Zhai Meng Wu Yupeng Yan Yuxiu Sun Zhiguo Zhang |
| author_sort | Yubo Li |
| collection | DOAJ |
| description | ObjectiveThis study aims to investigate the composition and function of the gut microbiome in long-term depression using an 8-week chronic unpredictable mild stress (CUMS) rat model.Materials and methodsAnimals were sacrificed after either 4 weeks or 8 weeks under CUMS to mimic long-term depression in humans. The gut microbiome was analyzed to identify potential depression-related gut microbes, and the fecal metabolome was analyzed to detect their functional metabolites. The correlations between altered gut microbes and metabolites in the long-term depression rats were explored. The crucial metabolic pathways related to long-term depression were uncovered through enrichment analysis based on these gut microbes and metabolites.ResultsThe microbial composition of long-term depression (8-week CUMS) showed decreased species richness indices and different profiles compared with the control group and the 4-week CUMS group, characterized by disturbance of Alistipes indistinctus, Bacteroides ovatus, and Alistipes senegalensis at the species level. Additionally, long-term depression was associated with disturbances in fecal metabolomics. D-pinitol was the only increased metabolite in the 8-week CUMS group among the top 10 differential metabolites, while the top 3 decreased metabolites in the long-term depression rats included indoxyl sulfate, trimethylaminen-oxide, and 3 alpha,7 alpha-dihydroxy-12-oxocholanoic acid. The disordered fecal metabolomics in the long-term depression rats mainly involved the biosynthesis of pantothenate, CoA, valine, leucine and isoleucine.ConclusionOur findings suggest that the gut microbiome may participate in the long-term development of depression, and the mechanism may be related to the regulation of gut metabolism. |
| first_indexed | 2024-04-09T21:25:46Z |
| format | Article |
| id | doaj.art-4c1f6c2642af4ecaa00dcebecb164b4b |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-09T21:25:46Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-4c1f6c2642af4ecaa00dcebecb164b4b2023-03-27T13:48:20ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-03-011310.3389/fcimb.2023.11162771116277Alteration of the gut microbiome and correlated metabolism in a rat model of long-term depressionYubo Li0Junling Li1Ran Cheng2Haixia Liu3Yukun Zhao4Yanjun Liu5Yanjing Chen6Zhibo Sun7Zhiguang Zhai8Meng Wu9Yupeng Yan10Yuxiu Sun11Zhiguo Zhang12Institute of Basic Theory for Traditional Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing, ChinaSchool of Traditional Chinese Medicine, Capital Medical University, Beijing, ChinaDepartment of Gynaecology and Obstetrics, Hangzhou Traditional Chinese Medicine (TCM) Hospital Affiliated to Zhejiang Chinese Medical University, Hangzhou, ChinaInstitute of Basic Theory for Traditional Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing, ChinaInstitute of Basic Theory for Traditional Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing, ChinaInstitute of Basic Theory for Traditional Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing, ChinaInstitute of Basic Theory for Traditional Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing, ChinaInstitute of Basic Theory for Traditional Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing, ChinaInstitute of Basic Theory for Traditional Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing, ChinaInstitute of Basic Theory for Traditional Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing, ChinaInstitute of Basic Theory for Traditional Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing, ChinaInstitute of Basic Theory for Traditional Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing, ChinaInstitute of Basic Theory for Traditional Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing, ChinaObjectiveThis study aims to investigate the composition and function of the gut microbiome in long-term depression using an 8-week chronic unpredictable mild stress (CUMS) rat model.Materials and methodsAnimals were sacrificed after either 4 weeks or 8 weeks under CUMS to mimic long-term depression in humans. The gut microbiome was analyzed to identify potential depression-related gut microbes, and the fecal metabolome was analyzed to detect their functional metabolites. The correlations between altered gut microbes and metabolites in the long-term depression rats were explored. The crucial metabolic pathways related to long-term depression were uncovered through enrichment analysis based on these gut microbes and metabolites.ResultsThe microbial composition of long-term depression (8-week CUMS) showed decreased species richness indices and different profiles compared with the control group and the 4-week CUMS group, characterized by disturbance of Alistipes indistinctus, Bacteroides ovatus, and Alistipes senegalensis at the species level. Additionally, long-term depression was associated with disturbances in fecal metabolomics. D-pinitol was the only increased metabolite in the 8-week CUMS group among the top 10 differential metabolites, while the top 3 decreased metabolites in the long-term depression rats included indoxyl sulfate, trimethylaminen-oxide, and 3 alpha,7 alpha-dihydroxy-12-oxocholanoic acid. The disordered fecal metabolomics in the long-term depression rats mainly involved the biosynthesis of pantothenate, CoA, valine, leucine and isoleucine.ConclusionOur findings suggest that the gut microbiome may participate in the long-term development of depression, and the mechanism may be related to the regulation of gut metabolism.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1116277/fulllong-term depressionCUMSgut microbiomemetabolomerat |
| spellingShingle | Yubo Li Junling Li Ran Cheng Haixia Liu Yukun Zhao Yanjun Liu Yanjing Chen Zhibo Sun Zhiguang Zhai Meng Wu Yupeng Yan Yuxiu Sun Zhiguo Zhang Alteration of the gut microbiome and correlated metabolism in a rat model of long-term depression Frontiers in Cellular and Infection Microbiology long-term depression CUMS gut microbiome metabolome rat |
| title | Alteration of the gut microbiome and correlated metabolism in a rat model of long-term depression |
| title_full | Alteration of the gut microbiome and correlated metabolism in a rat model of long-term depression |
| title_fullStr | Alteration of the gut microbiome and correlated metabolism in a rat model of long-term depression |
| title_full_unstemmed | Alteration of the gut microbiome and correlated metabolism in a rat model of long-term depression |
| title_short | Alteration of the gut microbiome and correlated metabolism in a rat model of long-term depression |
| title_sort | alteration of the gut microbiome and correlated metabolism in a rat model of long term depression |
| topic | long-term depression CUMS gut microbiome metabolome rat |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1116277/full |
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