Retracted: MiR‐191‐5p inhibits lung adenocarcinoma by repressing SATB1 to inhibit Wnt pathway
Abstract Background To investigate the function of miR‐191‐5p in lung adenocarcinoma and its possible mechanism. Methods QRT‐PCR was adopted for the detection of the expression levels of miR‐191‐5p and SATB1 (HGNC: 10541). The effects of miR‐191‐5p and SATB1 on cell proliferation and migration were...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Molecular Genetics & Genomic Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/mgg3.1043 |
| _version_ | 1811215827501842432 |
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| author | Lai‐yong Zhou Fu‐wei Zhang Jian Tong Fang Liu |
| author_facet | Lai‐yong Zhou Fu‐wei Zhang Jian Tong Fang Liu |
| author_sort | Lai‐yong Zhou |
| collection | DOAJ |
| description | Abstract Background To investigate the function of miR‐191‐5p in lung adenocarcinoma and its possible mechanism. Methods QRT‐PCR was adopted for the detection of the expression levels of miR‐191‐5p and SATB1 (HGNC: 10541). The effects of miR‐191‐5p and SATB1 on cell proliferation and migration were examined through the CCK‐8 and Transwell assays. Subsequently, the binding relationships between miR‐191‐5p and SATB1 were confirmed by dual‐luciferase reporter gene assay. Finally, the potential mechanisms of action of miR‐191‐5p were explored through a serious of in vivo and in vitro experiments. Results Lung adenocarcinoma patients had a notably lower expression level of miR‐191‐5p than controls, patients with metastasis had a lower level than those without metastasis, and the level in patients with lung adenocarcinoma in stage III‐IV was lower than that in patients with lung adenocarcinoma in stage I‐II. Overexpression of miR‐191‐5p repressed the migration and proliferation of lung cancer A549/H1650 cells. According to the reporter gene assay, miR‐191‐5p could bind to SATB1. Besides, SATB1 was significantly overexpressed in cancer tissues of patients with lung adenocarcinoma, and SATB1 overexpression accelerated the migration and proliferation of A549/H1650 cells and reversed inhibition on cell migration and proliferation by miR‐191‐5p. Conclusion Overexpression of miR‐191‐5p is capable of blocking the migration and proliferation of lung cancer cells, and its mechanism may be through targeting SATB1 thus downregulating Wnt signaling. |
| first_indexed | 2024-04-12T06:28:59Z |
| format | Article |
| id | doaj.art-4c20957b192e48178061f7059f017877 |
| institution | Directory Open Access Journal |
| issn | 2324-9269 |
| language | English |
| last_indexed | 2024-04-12T06:28:59Z |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Molecular Genetics & Genomic Medicine |
| spelling | doaj.art-4c20957b192e48178061f7059f0178772022-12-22T03:44:04ZengWileyMolecular Genetics & Genomic Medicine2324-92692020-01-0181n/an/a10.1002/mgg3.1043Retracted: MiR‐191‐5p inhibits lung adenocarcinoma by repressing SATB1 to inhibit Wnt pathwayLai‐yong Zhou0Fu‐wei Zhang1Jian Tong2Fang Liu3Department of Cardiothoracic surgery The People's Hospital of Bao’an Shenzhen The Affiliated Bao’an Hospital of Southern Medical University Shenzhen ChinaDepartment of Cardiothoracic surgery Zhujiang Hospital Southern Medical University Guangzhou ChinaDepartment of Cardiothoracic surgery Zhujiang Hospital Southern Medical University Guangzhou ChinaDepartment of Pathology The People's Hospital of Bao’an Shenzhen The Affiliated Bao’an Hospital of Southern Medical University Shenzhen ChinaAbstract Background To investigate the function of miR‐191‐5p in lung adenocarcinoma and its possible mechanism. Methods QRT‐PCR was adopted for the detection of the expression levels of miR‐191‐5p and SATB1 (HGNC: 10541). The effects of miR‐191‐5p and SATB1 on cell proliferation and migration were examined through the CCK‐8 and Transwell assays. Subsequently, the binding relationships between miR‐191‐5p and SATB1 were confirmed by dual‐luciferase reporter gene assay. Finally, the potential mechanisms of action of miR‐191‐5p were explored through a serious of in vivo and in vitro experiments. Results Lung adenocarcinoma patients had a notably lower expression level of miR‐191‐5p than controls, patients with metastasis had a lower level than those without metastasis, and the level in patients with lung adenocarcinoma in stage III‐IV was lower than that in patients with lung adenocarcinoma in stage I‐II. Overexpression of miR‐191‐5p repressed the migration and proliferation of lung cancer A549/H1650 cells. According to the reporter gene assay, miR‐191‐5p could bind to SATB1. Besides, SATB1 was significantly overexpressed in cancer tissues of patients with lung adenocarcinoma, and SATB1 overexpression accelerated the migration and proliferation of A549/H1650 cells and reversed inhibition on cell migration and proliferation by miR‐191‐5p. Conclusion Overexpression of miR‐191‐5p is capable of blocking the migration and proliferation of lung cancer cells, and its mechanism may be through targeting SATB1 thus downregulating Wnt signaling.https://doi.org/10.1002/mgg3.1043lung adenocarcinomametastatic lung cancermiR‐191‐5pSATB1 |
| spellingShingle | Lai‐yong Zhou Fu‐wei Zhang Jian Tong Fang Liu Retracted: MiR‐191‐5p inhibits lung adenocarcinoma by repressing SATB1 to inhibit Wnt pathway Molecular Genetics & Genomic Medicine lung adenocarcinoma metastatic lung cancer miR‐191‐5p SATB1 |
| title | Retracted: MiR‐191‐5p inhibits lung adenocarcinoma by repressing SATB1 to inhibit Wnt pathway |
| title_full | Retracted: MiR‐191‐5p inhibits lung adenocarcinoma by repressing SATB1 to inhibit Wnt pathway |
| title_fullStr | Retracted: MiR‐191‐5p inhibits lung adenocarcinoma by repressing SATB1 to inhibit Wnt pathway |
| title_full_unstemmed | Retracted: MiR‐191‐5p inhibits lung adenocarcinoma by repressing SATB1 to inhibit Wnt pathway |
| title_short | Retracted: MiR‐191‐5p inhibits lung adenocarcinoma by repressing SATB1 to inhibit Wnt pathway |
| title_sort | retracted mir 191 5p inhibits lung adenocarcinoma by repressing satb1 to inhibit wnt pathway |
| topic | lung adenocarcinoma metastatic lung cancer miR‐191‐5p SATB1 |
| url | https://doi.org/10.1002/mgg3.1043 |
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