Nanotheranostic Trojan Horse for visualization and photo-immunotherapy of multidrug-resistant bacterial infection
Abstract Effective diagnosis and therapy for bacterial infections, especially those caused by multidrug-resistant (MDR) species, greatly challenge current antimicrobial stewardship. Monocytes, which can chemotactically migrate from the blood to infection site and elicit a robust infection infiltrati...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-12-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-023-02267-6 |
| _version_ | 1827399325414588416 |
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| author | Xin Pang Haohang Xu Qishun Geng Yu Han Huiya Zhang Heng Liu Xiao Zhang Mingsan Miao |
| author_facet | Xin Pang Haohang Xu Qishun Geng Yu Han Huiya Zhang Heng Liu Xiao Zhang Mingsan Miao |
| author_sort | Xin Pang |
| collection | DOAJ |
| description | Abstract Effective diagnosis and therapy for bacterial infections, especially those caused by multidrug-resistant (MDR) species, greatly challenge current antimicrobial stewardship. Monocytes, which can chemotactically migrate from the blood to infection site and elicit a robust infection infiltration, provide a golden opportunity for bacterial theranostics. Here, a nano-Trojan Horse was facilely engineered using mannose-functionalized manganese-eumelanin coordination nanoparticles (denoted as MP-MENP) for precise two-step localization and potent photothermal-immunotherapy of MDR bacterial infection. Taking advantage of the selective recognition between mannose and inflammation-associated monocytes, the MP-MENP could be passively piggybacked to infection site by circulating monocytes, and also actively target infiltrated monocytes that are already accumulated in infection microenvironment. Such dual-pronged targeting enabled an efficient imaging diagnosis of bacterial infection. Upon laser irradiation, the MP-MENP robustly produced local hyperemia to ablate bacteria, both extracellularly and intracellularly. Further combined with photothermal therapy-induced immunogenic cell death and MP-MENP-mediated macrophage reprogramming, the immunosuppressive infection microenvironment was significantly relieved, allowing an enhanced antibacterial immunity. Collectively, the proposed nanotheranostic Trojan Horse, which integrates dual-pronged targeting, precise imaging diagnosis, and high-performance photothermal immunotherapy, promises a new way for complete eradication of MDR bacterial infection. |
| first_indexed | 2024-03-08T19:44:17Z |
| format | Article |
| id | doaj.art-4c20ab18431c438cb23ecd378a2b8ec3 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-03-08T19:44:17Z |
| publishDate | 2023-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-4c20ab18431c438cb23ecd378a2b8ec32023-12-24T12:28:13ZengBMCJournal of Nanobiotechnology1477-31552023-12-0121111510.1186/s12951-023-02267-6Nanotheranostic Trojan Horse for visualization and photo-immunotherapy of multidrug-resistant bacterial infectionXin Pang0Haohang Xu1Qishun Geng2Yu Han3Huiya Zhang4Heng Liu5Xiao Zhang6Mingsan Miao7School of Pharmacy, Henan University of Traditional Chinese MedicineSchool of Pharmacy, Henan University of Traditional Chinese MedicineChina-Japan Friendship Hospital (Institute of Clinical Medical Sciences), Chinese Academy of Medical Sciences & Peking Union Medical CollegeJoint Institute of Management and Science University, Henan University of Traditional Chinese MedicineJoint Institute of Management and Science University, Henan University of Traditional Chinese MedicineDepartment of Radiology, PLA Rocket Force Characteristic Medical CenterSchool of Pharmacy, Henan University of Traditional Chinese MedicineAcademy of Chinese Medical Sciences, Henan University of Traditional Chinese MedicineAbstract Effective diagnosis and therapy for bacterial infections, especially those caused by multidrug-resistant (MDR) species, greatly challenge current antimicrobial stewardship. Monocytes, which can chemotactically migrate from the blood to infection site and elicit a robust infection infiltration, provide a golden opportunity for bacterial theranostics. Here, a nano-Trojan Horse was facilely engineered using mannose-functionalized manganese-eumelanin coordination nanoparticles (denoted as MP-MENP) for precise two-step localization and potent photothermal-immunotherapy of MDR bacterial infection. Taking advantage of the selective recognition between mannose and inflammation-associated monocytes, the MP-MENP could be passively piggybacked to infection site by circulating monocytes, and also actively target infiltrated monocytes that are already accumulated in infection microenvironment. Such dual-pronged targeting enabled an efficient imaging diagnosis of bacterial infection. Upon laser irradiation, the MP-MENP robustly produced local hyperemia to ablate bacteria, both extracellularly and intracellularly. Further combined with photothermal therapy-induced immunogenic cell death and MP-MENP-mediated macrophage reprogramming, the immunosuppressive infection microenvironment was significantly relieved, allowing an enhanced antibacterial immunity. Collectively, the proposed nanotheranostic Trojan Horse, which integrates dual-pronged targeting, precise imaging diagnosis, and high-performance photothermal immunotherapy, promises a new way for complete eradication of MDR bacterial infection.https://doi.org/10.1186/s12951-023-02267-6AntibacterialMonocyte-hitchhikingPhoto-immunotherapyTheranosticsBacterial Infection |
| spellingShingle | Xin Pang Haohang Xu Qishun Geng Yu Han Huiya Zhang Heng Liu Xiao Zhang Mingsan Miao Nanotheranostic Trojan Horse for visualization and photo-immunotherapy of multidrug-resistant bacterial infection Journal of Nanobiotechnology Antibacterial Monocyte-hitchhiking Photo-immunotherapy Theranostics Bacterial Infection |
| title | Nanotheranostic Trojan Horse for visualization and photo-immunotherapy of multidrug-resistant bacterial infection |
| title_full | Nanotheranostic Trojan Horse for visualization and photo-immunotherapy of multidrug-resistant bacterial infection |
| title_fullStr | Nanotheranostic Trojan Horse for visualization and photo-immunotherapy of multidrug-resistant bacterial infection |
| title_full_unstemmed | Nanotheranostic Trojan Horse for visualization and photo-immunotherapy of multidrug-resistant bacterial infection |
| title_short | Nanotheranostic Trojan Horse for visualization and photo-immunotherapy of multidrug-resistant bacterial infection |
| title_sort | nanotheranostic trojan horse for visualization and photo immunotherapy of multidrug resistant bacterial infection |
| topic | Antibacterial Monocyte-hitchhiking Photo-immunotherapy Theranostics Bacterial Infection |
| url | https://doi.org/10.1186/s12951-023-02267-6 |
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