Dissecting human trophoblast cell transcriptional heterogeneity in preeclampsia using single‐cell RNA sequencing
Abstract Objective PE is a pregnancy‐specific syndrome that affects 3%–5% of pregnant women. It often presents as new‐onset hypertension and proteinuria during the third trimester. PE progresses rapidly and may lead to serious complications, including the death of both mother and fetus. In low‐incom...
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| Format: | Article |
| Language: | English |
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Wiley
2021-08-01
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| Series: | Molecular Genetics & Genomic Medicine |
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| Online Access: | https://doi.org/10.1002/mgg3.1730 |
| _version_ | 1818603008790888448 |
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| author | Tao Zhang Qianqian Bian Yanchun Chen Xiaolin Wang Shaowei Yu Shunhua Liu Ping Ji Ling Li Mandakini Shrestha Shujun Dong Rong Guo Hong Zhang |
| author_facet | Tao Zhang Qianqian Bian Yanchun Chen Xiaolin Wang Shaowei Yu Shunhua Liu Ping Ji Ling Li Mandakini Shrestha Shujun Dong Rong Guo Hong Zhang |
| author_sort | Tao Zhang |
| collection | DOAJ |
| description | Abstract Objective PE is a pregnancy‐specific syndrome that affects 3%–5% of pregnant women. It often presents as new‐onset hypertension and proteinuria during the third trimester. PE progresses rapidly and may lead to serious complications, including the death of both mother and fetus. In low‐income countries, PE is one of the main causes of maternal and child mortality. While the cause of PE is still debated, clinical and pathological studies suggest that the placenta plays an important role in the pathogenesis of PE. Materials and Methods In this single‐cell RNA‐sequencing (RNA‐seq) study, the placenta was taken from the designated position after cesarean section. We compared placental cell subsets and their transcriptional heterogeneity between preeclampsia and healthy pregnancies using the single‐cell RNA‐seq technology. A developmental trajectory of human trophoblasts was shown. Results Gene expression in endoplasmic reticulum signaling pathways in syncytiotrophoblast was upregulated in the PE group. The villi cytotrophoblasts (VCT) and extravillous trophoblasts were mainly involved in immune responses. Conclusion The placental immune function of patients with PE was altered. Proteasomes, spliceosomes, ribosomes, and mitochondria were abnormally active in the new VCT cell type. |
| first_indexed | 2024-12-16T13:16:21Z |
| format | Article |
| id | doaj.art-4c244da36d9e4ed89c419fb361ab5159 |
| institution | Directory Open Access Journal |
| issn | 2324-9269 |
| language | English |
| last_indexed | 2024-12-16T13:16:21Z |
| publishDate | 2021-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Molecular Genetics & Genomic Medicine |
| spelling | doaj.art-4c244da36d9e4ed89c419fb361ab51592022-12-21T22:30:28ZengWileyMolecular Genetics & Genomic Medicine2324-92692021-08-0198n/an/a10.1002/mgg3.1730Dissecting human trophoblast cell transcriptional heterogeneity in preeclampsia using single‐cell RNA sequencingTao Zhang0Qianqian Bian1Yanchun Chen2Xiaolin Wang3Shaowei Yu4Shunhua Liu5Ping Ji6Ling Li7Mandakini Shrestha8Shujun Dong9Rong Guo10Hong Zhang11Department of Obstetrics and Gynecology The Second Affiliated Hospital of Soochow University Suzhou ChinaDepartment of Nephrology Weifang Traditional Chinese Hospital Weifang Shandong ChinaDepartment of Histology and Embryology Basic Medical CollegeWeifang Medical University Weifang Shandong ChinaDepartment of Pathology Weifang Traditional Chinese Hospital Weifang Shandong ChinaDepartment of Obstetrics and Gynecology Weifang Traditional Chinese Hospital Weifang Shandong ChinaDepartment of Obstetrics and Gynecology Weifang Traditional Chinese Hospital Weifang Shandong ChinaDepartment of Obstetrics and Gynecology Weifang Traditional Chinese Hospital Weifang Shandong ChinaDepartment of Obstetrics and Gynecology Weifang Traditional Chinese Hospital Weifang Shandong ChinaDepartment of Obstetrics and Gynecology Third Affiliated Hospital of Guangzhou Medical University Guangzhou ChinaDepartment of Obstetrics and Gynecology Weifang Traditional Chinese Hospital Weifang Shandong ChinaDepartment of Obstetrics and Gynaecology The First Affiliated Hospital of Gannan Medical University Ganzhou Jiangxi ChinaDepartment of Obstetrics and Gynecology The Second Affiliated Hospital of Soochow University Suzhou ChinaAbstract Objective PE is a pregnancy‐specific syndrome that affects 3%–5% of pregnant women. It often presents as new‐onset hypertension and proteinuria during the third trimester. PE progresses rapidly and may lead to serious complications, including the death of both mother and fetus. In low‐income countries, PE is one of the main causes of maternal and child mortality. While the cause of PE is still debated, clinical and pathological studies suggest that the placenta plays an important role in the pathogenesis of PE. Materials and Methods In this single‐cell RNA‐sequencing (RNA‐seq) study, the placenta was taken from the designated position after cesarean section. We compared placental cell subsets and their transcriptional heterogeneity between preeclampsia and healthy pregnancies using the single‐cell RNA‐seq technology. A developmental trajectory of human trophoblasts was shown. Results Gene expression in endoplasmic reticulum signaling pathways in syncytiotrophoblast was upregulated in the PE group. The villi cytotrophoblasts (VCT) and extravillous trophoblasts were mainly involved in immune responses. Conclusion The placental immune function of patients with PE was altered. Proteasomes, spliceosomes, ribosomes, and mitochondria were abnormally active in the new VCT cell type.https://doi.org/10.1002/mgg3.1730extravillous trophoblastspreeclampsiasingle‐cell RNA sequencingsyncytiotrophoblastvilli cytotrophoblast |
| spellingShingle | Tao Zhang Qianqian Bian Yanchun Chen Xiaolin Wang Shaowei Yu Shunhua Liu Ping Ji Ling Li Mandakini Shrestha Shujun Dong Rong Guo Hong Zhang Dissecting human trophoblast cell transcriptional heterogeneity in preeclampsia using single‐cell RNA sequencing Molecular Genetics & Genomic Medicine extravillous trophoblasts preeclampsia single‐cell RNA sequencing syncytiotrophoblast villi cytotrophoblast |
| title | Dissecting human trophoblast cell transcriptional heterogeneity in preeclampsia using single‐cell RNA sequencing |
| title_full | Dissecting human trophoblast cell transcriptional heterogeneity in preeclampsia using single‐cell RNA sequencing |
| title_fullStr | Dissecting human trophoblast cell transcriptional heterogeneity in preeclampsia using single‐cell RNA sequencing |
| title_full_unstemmed | Dissecting human trophoblast cell transcriptional heterogeneity in preeclampsia using single‐cell RNA sequencing |
| title_short | Dissecting human trophoblast cell transcriptional heterogeneity in preeclampsia using single‐cell RNA sequencing |
| title_sort | dissecting human trophoblast cell transcriptional heterogeneity in preeclampsia using single cell rna sequencing |
| topic | extravillous trophoblasts preeclampsia single‐cell RNA sequencing syncytiotrophoblast villi cytotrophoblast |
| url | https://doi.org/10.1002/mgg3.1730 |
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