Novel monoclonal antibodies against Fiber-1 of duck adenovirus 3 and their B cell epitopes
Recently, the outbreak of the infection of Duck adenovirus 3 (DAdV-3) characterized by swelling and hemorrhagic liver and kidney has caused huge economic losses to duck industry since 2014 in China. To date, the B cell epitopes in the Fiber-1 protein and the underlying infection mechanism of DAdV-3...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-10-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.1003262/full |
| _version_ | 1798020735892979712 |
|---|---|
| author | Hongxia Shao Hongxia Shao Hongxia Shao Wenyuan Zhang Wenyuan Zhang Wenyuan Zhang Yun Lin Yun Lin Yun Lin Jing Xie Jing Xie Jing Xie Dan Ren Dan Ren Dan Ren Quan Xie Quan Xie Quan Xie Tuofan Li Tuofan Li Tuofan Li Zhimin Wan Zhimin Wan Zhimin Wan Aijian Qin Aijian Qin Aijian Qin Jianqiang Ye Jianqiang Ye Jianqiang Ye |
| author_facet | Hongxia Shao Hongxia Shao Hongxia Shao Wenyuan Zhang Wenyuan Zhang Wenyuan Zhang Yun Lin Yun Lin Yun Lin Jing Xie Jing Xie Jing Xie Dan Ren Dan Ren Dan Ren Quan Xie Quan Xie Quan Xie Tuofan Li Tuofan Li Tuofan Li Zhimin Wan Zhimin Wan Zhimin Wan Aijian Qin Aijian Qin Aijian Qin Jianqiang Ye Jianqiang Ye Jianqiang Ye |
| author_sort | Hongxia Shao |
| collection | DOAJ |
| description | Recently, the outbreak of the infection of Duck adenovirus 3 (DAdV-3) characterized by swelling and hemorrhagic liver and kidney has caused huge economic losses to duck industry since 2014 in China. To date, the B cell epitopes in the Fiber-1 protein and the underlying infection mechanism of DAdV-3 have not been investigated. In this study, the recombinant Fiber-1 protein was first expressed in E. coli and six novel monoclonal antibodies (mAbs) against Fiber-1 were generated, designated as 1D8, 1E6, 3G6, 4G1, 4G2, and 6F10, respectively. Moreover, mAbs 3G6 and 6F10 could efficiently immunoprecipitate the Fiber-1 in LMH cells infected with DAdV-3 or transfected with pcDNA3.1-Fiber-1. Notably, mAbs 3G6 and 4G2 also showed certain neutralizing activity against DAdV-3 infection in vitro. Epitopes mapping revealed that the B cell epitope recognized by 6F10, 3G6, 4G1, 1D8, 4G2, and 1E6 was located in 34-66aa, 67-99aa, 64-296aa, 297-329aa, 330-362aa, and 363-395aa, respectively. Sequence alignments further found that the six epitopes recognized by these mAbs were highly conserved among different DAdV-3 isolates. The generated mAbs specific to Fiber-1 and their defined epitopes provide powerful tools for establishing rapid and efficient diagnostics for the detection of DAdV-3 and pave the way for further studying on the critical role of Fiber-1 in mediating the infection of DAdV-3. |
| first_indexed | 2024-04-11T17:02:27Z |
| format | Article |
| id | doaj.art-4c260d5f21d44d5cb827196626cd899f |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-11T17:02:27Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-4c260d5f21d44d5cb827196626cd899f2022-12-22T04:13:08ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-10-01910.3389/fvets.2022.10032621003262Novel monoclonal antibodies against Fiber-1 of duck adenovirus 3 and their B cell epitopesHongxia Shao0Hongxia Shao1Hongxia Shao2Wenyuan Zhang3Wenyuan Zhang4Wenyuan Zhang5Yun Lin6Yun Lin7Yun Lin8Jing Xie9Jing Xie10Jing Xie11Dan Ren12Dan Ren13Dan Ren14Quan Xie15Quan Xie16Quan Xie17Tuofan Li18Tuofan Li19Tuofan Li20Zhimin Wan21Zhimin Wan22Zhimin Wan23Aijian Qin24Aijian Qin25Aijian Qin26Jianqiang Ye27Jianqiang Ye28Jianqiang Ye29Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, ChinaKey Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaJiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, ChinaRecently, the outbreak of the infection of Duck adenovirus 3 (DAdV-3) characterized by swelling and hemorrhagic liver and kidney has caused huge economic losses to duck industry since 2014 in China. To date, the B cell epitopes in the Fiber-1 protein and the underlying infection mechanism of DAdV-3 have not been investigated. In this study, the recombinant Fiber-1 protein was first expressed in E. coli and six novel monoclonal antibodies (mAbs) against Fiber-1 were generated, designated as 1D8, 1E6, 3G6, 4G1, 4G2, and 6F10, respectively. Moreover, mAbs 3G6 and 6F10 could efficiently immunoprecipitate the Fiber-1 in LMH cells infected with DAdV-3 or transfected with pcDNA3.1-Fiber-1. Notably, mAbs 3G6 and 4G2 also showed certain neutralizing activity against DAdV-3 infection in vitro. Epitopes mapping revealed that the B cell epitope recognized by 6F10, 3G6, 4G1, 1D8, 4G2, and 1E6 was located in 34-66aa, 67-99aa, 64-296aa, 297-329aa, 330-362aa, and 363-395aa, respectively. Sequence alignments further found that the six epitopes recognized by these mAbs were highly conserved among different DAdV-3 isolates. The generated mAbs specific to Fiber-1 and their defined epitopes provide powerful tools for establishing rapid and efficient diagnostics for the detection of DAdV-3 and pave the way for further studying on the critical role of Fiber-1 in mediating the infection of DAdV-3.https://www.frontiersin.org/articles/10.3389/fvets.2022.1003262/fullduck adenovirus 3Fiber-1monoclonal antibodiesIFAWestern blotimmunoprecipitation |
| spellingShingle | Hongxia Shao Hongxia Shao Hongxia Shao Wenyuan Zhang Wenyuan Zhang Wenyuan Zhang Yun Lin Yun Lin Yun Lin Jing Xie Jing Xie Jing Xie Dan Ren Dan Ren Dan Ren Quan Xie Quan Xie Quan Xie Tuofan Li Tuofan Li Tuofan Li Zhimin Wan Zhimin Wan Zhimin Wan Aijian Qin Aijian Qin Aijian Qin Jianqiang Ye Jianqiang Ye Jianqiang Ye Novel monoclonal antibodies against Fiber-1 of duck adenovirus 3 and their B cell epitopes Frontiers in Veterinary Science duck adenovirus 3 Fiber-1 monoclonal antibodies IFA Western blot immunoprecipitation |
| title | Novel monoclonal antibodies against Fiber-1 of duck adenovirus 3 and their B cell epitopes |
| title_full | Novel monoclonal antibodies against Fiber-1 of duck adenovirus 3 and their B cell epitopes |
| title_fullStr | Novel monoclonal antibodies against Fiber-1 of duck adenovirus 3 and their B cell epitopes |
| title_full_unstemmed | Novel monoclonal antibodies against Fiber-1 of duck adenovirus 3 and their B cell epitopes |
| title_short | Novel monoclonal antibodies against Fiber-1 of duck adenovirus 3 and their B cell epitopes |
| title_sort | novel monoclonal antibodies against fiber 1 of duck adenovirus 3 and their b cell epitopes |
| topic | duck adenovirus 3 Fiber-1 monoclonal antibodies IFA Western blot immunoprecipitation |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.1003262/full |
| work_keys_str_mv | AT hongxiashao novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT hongxiashao novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT hongxiashao novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT wenyuanzhang novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT wenyuanzhang novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT wenyuanzhang novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT yunlin novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT yunlin novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT yunlin novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT jingxie novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT jingxie novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT jingxie novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT danren novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT danren novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT danren novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT quanxie novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT quanxie novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT quanxie novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT tuofanli novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT tuofanli novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT tuofanli novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT zhiminwan novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT zhiminwan novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT zhiminwan novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT aijianqin novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT aijianqin novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT aijianqin novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT jianqiangye novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT jianqiangye novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes AT jianqiangye novelmonoclonalantibodiesagainstfiber1ofduckadenovirus3andtheirbcellepitopes |