Osteogenic activity of polyetheretherketone/58S bioactive glasses bone implant
Objective To explore the osteogenic activity of polyetheretherketone/58S bioactive glasses (PEEK/58S) as a kind of bone implant. Methods MG-63 osteoblasts were cultured on PEEK and PEEK/58S surfaces for predetermined time. Then MG-63 osteoblast attachment and spreading condition were observed by s...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2021-02-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200730.pdf |
| _version_ | 1827390584493441024 |
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| author | LIU Lyu-hua, ZHENG Yan-yan, ZHANG Li-fang, XIONG Cheng-dong |
| author_facet | LIU Lyu-hua, ZHENG Yan-yan, ZHANG Li-fang, XIONG Cheng-dong |
| author_sort | LIU Lyu-hua, ZHENG Yan-yan, ZHANG Li-fang, XIONG Cheng-dong |
| collection | DOAJ |
| description | Objective To explore the osteogenic activity of polyetheretherketone/58S bioactive glasses (PEEK/58S) as a kind of bone implant. Methods MG-63 osteoblasts were cultured on PEEK and PEEK/58S surfaces for predetermined time. Then MG-63 osteoblast attachment and spreading condition were observed by scanning electron microscopy (SEM). The proliferation activity of MG-63 osteoblast was evaluated by CCK-8 (cell counting kit). Alkaline phosphatase (ALP) activity was measured to determine the osteogenic differentiation of MG-63 osteoblast. The mRNA expression levels of osteogenesis-related genes ALP, BMP-2, COL-I, OCN and OPN were detected using real-time fluorescent quantitative PCR. Osseointegration of PEEK and PEEK/58S bone implant was evaluated by histological analysis and biomechanical pull-out tests after implantation in a rat tibia model for 12 weeks. Results Compared with the PEEK, the adhesion and spreading of MG-63 osteoblast was more favorable. The proliferation activity and ALP activity of MG-63 osteoblast was significantly promoted (P＜0.05). Also, the mRNA expression of ALP, BMP-2, COL-I, OCN and OPN was significantly up-regulated (P＜0.05). In vivo experiments showed that PEEK/58S exhibited better osseointegration than pure PEEK. Conclusions PEEK/58S bone implant significantly improves the attachment, spreading, proliferation and differentiation of osteoblast and enhances the peri-implant osseointegration, so it is a potential novel orthopedic material for clinical use. |
| first_indexed | 2024-03-08T16:54:50Z |
| format | Article |
| id | doaj.art-4c263ddc1b604c7cb5cf2db576b025de |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:54:50Z |
| publishDate | 2021-02-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-4c263ddc1b604c7cb5cf2db576b025de2024-01-05T03:04:29ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252021-02-01412209213Osteogenic activity of polyetheretherketone/58S bioactive glasses bone implantLIU Lyu-hua, ZHENG Yan-yan, ZHANG Li-fang, XIONG Cheng-dong01. School of Basic Medical Sciences, North Sichuan Medical College, Nanchong 637000; ;2. Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041, ChinaObjective To explore the osteogenic activity of polyetheretherketone/58S bioactive glasses (PEEK/58S) as a kind of bone implant. Methods MG-63 osteoblasts were cultured on PEEK and PEEK/58S surfaces for predetermined time. Then MG-63 osteoblast attachment and spreading condition were observed by scanning electron microscopy (SEM). The proliferation activity of MG-63 osteoblast was evaluated by CCK-8 (cell counting kit). Alkaline phosphatase (ALP) activity was measured to determine the osteogenic differentiation of MG-63 osteoblast. The mRNA expression levels of osteogenesis-related genes ALP, BMP-2, COL-I, OCN and OPN were detected using real-time fluorescent quantitative PCR. Osseointegration of PEEK and PEEK/58S bone implant was evaluated by histological analysis and biomechanical pull-out tests after implantation in a rat tibia model for 12 weeks. Results Compared with the PEEK, the adhesion and spreading of MG-63 osteoblast was more favorable. The proliferation activity and ALP activity of MG-63 osteoblast was significantly promoted (P＜0.05). Also, the mRNA expression of ALP, BMP-2, COL-I, OCN and OPN was significantly up-regulated (P＜0.05). In vivo experiments showed that PEEK/58S exhibited better osseointegration than pure PEEK. Conclusions PEEK/58S bone implant significantly improves the attachment, spreading, proliferation and differentiation of osteoblast and enhances the peri-implant osseointegration, so it is a potential novel orthopedic material for clinical use.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200730.pdfpolyetheretherketone|58s bioactive glasses|bioactivity|osteogenic activity|osseointegration |
| spellingShingle | LIU Lyu-hua, ZHENG Yan-yan, ZHANG Li-fang, XIONG Cheng-dong Osteogenic activity of polyetheretherketone/58S bioactive glasses bone implant Jichu yixue yu linchuang polyetheretherketone|58s bioactive glasses|bioactivity|osteogenic activity|osseointegration |
| title | Osteogenic activity of polyetheretherketone/58S bioactive glasses bone implant |
| title_full | Osteogenic activity of polyetheretherketone/58S bioactive glasses bone implant |
| title_fullStr | Osteogenic activity of polyetheretherketone/58S bioactive glasses bone implant |
| title_full_unstemmed | Osteogenic activity of polyetheretherketone/58S bioactive glasses bone implant |
| title_short | Osteogenic activity of polyetheretherketone/58S bioactive glasses bone implant |
| title_sort | osteogenic activity of polyetheretherketone 58s bioactive glasses bone implant |
| topic | polyetheretherketone|58s bioactive glasses|bioactivity|osteogenic activity|osseointegration |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200730.pdf |
| work_keys_str_mv | AT liulyuhuazhengyanyanzhanglifangxiongchengdong osteogenicactivityofpolyetheretherketone58sbioactiveglassesboneimplant |