Transformation of sugarcane molasses into fructooligosaccharides with enhanced prebiotic activity using whole-cell biocatalysts from Aureobasidium pullulans FRR 5284 and an invertase-deficient Saccharomyces cerevisiae 1403-7A
Abstract Fructooligosaccharides (FOS) can be used as feed prebiotics, but are limited by high production costs. In this study, low-cost sugarcane molasses was used to produce whole-cell biocatalysts containing transfructosylating enzymes by Aureobasidium pullulans FRR 5284, followed by FOS productio...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-09-01
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| Series: | Bioresources and Bioprocessing |
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| Online Access: | https://doi.org/10.1186/s40643-021-00438-7 |
| _version_ | 1819118538302947328 |
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| author | Most Sheauly Khatun Morteza Hassanpour Solange I. Mussatto Mark D. Harrison Robert E. Speight Ian M. O’Hara Zhanying Zhang |
| author_facet | Most Sheauly Khatun Morteza Hassanpour Solange I. Mussatto Mark D. Harrison Robert E. Speight Ian M. O’Hara Zhanying Zhang |
| author_sort | Most Sheauly Khatun |
| collection | DOAJ |
| description | Abstract Fructooligosaccharides (FOS) can be used as feed prebiotics, but are limited by high production costs. In this study, low-cost sugarcane molasses was used to produce whole-cell biocatalysts containing transfructosylating enzymes by Aureobasidium pullulans FRR 5284, followed by FOS production from molasses using the whole-cells of A. pullulans. A. pullulans in molasses-based medium produced cells and broth with a total transfructosylating activity of 123.6 U/mL compared to 61.0 and 85.8 U/mL in synthetic molasses-based and sucrose-based media, respectively. It was found that inclusion of glucose in sucrose medium reduced both transfructosylating and hydrolytic activities of the produced cells and broth. With the use of pure glucose medium, cells and broth had very low levels of transfructosylating activities and hydrolytic activities were not detected. These results indicated that A. pullulans FRR 5284 produced both constitutive and inducible enzymes in sucrose-rich media, such as molasses while it only produced constitutive enzymes in the glucose media. Furthermore, treatment of FOS solutions generated from sucrose-rich solutions using an invertase-deficient Saccharomyces yeast converted glucose to ethanol and acetic acid and improved FOS content in total sugars by 20–30%. Treated FOS derived from molasses improved the in vitro growth of nine probiotic strains by 9–63% compared to a commercial FOS in 12 h incubation. This study demonstrated the potential of using molasses to produce FOS for feed application. |
| first_indexed | 2024-12-22T05:50:28Z |
| format | Article |
| id | doaj.art-4c272fe8ec8a4698890044cc16298754 |
| institution | Directory Open Access Journal |
| issn | 2197-4365 |
| language | English |
| last_indexed | 2024-12-22T05:50:28Z |
| publishDate | 2021-09-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bioresources and Bioprocessing |
| spelling | doaj.art-4c272fe8ec8a4698890044cc162987542022-12-21T18:36:52ZengSpringerOpenBioresources and Bioprocessing2197-43652021-09-018111210.1186/s40643-021-00438-7Transformation of sugarcane molasses into fructooligosaccharides with enhanced prebiotic activity using whole-cell biocatalysts from Aureobasidium pullulans FRR 5284 and an invertase-deficient Saccharomyces cerevisiae 1403-7AMost Sheauly Khatun0Morteza Hassanpour1Solange I. Mussatto2Mark D. Harrison3Robert E. Speight4Ian M. O’Hara5Zhanying Zhang6Centre for Agriculture and the Bioeconomy, Faculty of Science, Queensland University of TechnologyCentre for Agriculture and the Bioeconomy, Faculty of Science, Queensland University of TechnologyDepartment of Biotechnology and Biomedicine, Technical University of DenmarkCentre for Agriculture and the Bioeconomy, Faculty of Science, Queensland University of TechnologyCentre for Agriculture and the Bioeconomy, Faculty of Science, Queensland University of TechnologyCentre for Agriculture and the Bioeconomy, Faculty of Science, Queensland University of TechnologyCentre for Agriculture and the Bioeconomy, Faculty of Science, Queensland University of TechnologyAbstract Fructooligosaccharides (FOS) can be used as feed prebiotics, but are limited by high production costs. In this study, low-cost sugarcane molasses was used to produce whole-cell biocatalysts containing transfructosylating enzymes by Aureobasidium pullulans FRR 5284, followed by FOS production from molasses using the whole-cells of A. pullulans. A. pullulans in molasses-based medium produced cells and broth with a total transfructosylating activity of 123.6 U/mL compared to 61.0 and 85.8 U/mL in synthetic molasses-based and sucrose-based media, respectively. It was found that inclusion of glucose in sucrose medium reduced both transfructosylating and hydrolytic activities of the produced cells and broth. With the use of pure glucose medium, cells and broth had very low levels of transfructosylating activities and hydrolytic activities were not detected. These results indicated that A. pullulans FRR 5284 produced both constitutive and inducible enzymes in sucrose-rich media, such as molasses while it only produced constitutive enzymes in the glucose media. Furthermore, treatment of FOS solutions generated from sucrose-rich solutions using an invertase-deficient Saccharomyces yeast converted glucose to ethanol and acetic acid and improved FOS content in total sugars by 20–30%. Treated FOS derived from molasses improved the in vitro growth of nine probiotic strains by 9–63% compared to a commercial FOS in 12 h incubation. This study demonstrated the potential of using molasses to produce FOS for feed application.https://doi.org/10.1186/s40643-021-00438-7Sugarcane molassesA. pullulansTransfructosylating activityFructooligosaccharidesPrebioticsProbiotics |
| spellingShingle | Most Sheauly Khatun Morteza Hassanpour Solange I. Mussatto Mark D. Harrison Robert E. Speight Ian M. O’Hara Zhanying Zhang Transformation of sugarcane molasses into fructooligosaccharides with enhanced prebiotic activity using whole-cell biocatalysts from Aureobasidium pullulans FRR 5284 and an invertase-deficient Saccharomyces cerevisiae 1403-7A Bioresources and Bioprocessing Sugarcane molasses A. pullulans Transfructosylating activity Fructooligosaccharides Prebiotics Probiotics |
| title | Transformation of sugarcane molasses into fructooligosaccharides with enhanced prebiotic activity using whole-cell biocatalysts from Aureobasidium pullulans FRR 5284 and an invertase-deficient Saccharomyces cerevisiae 1403-7A |
| title_full | Transformation of sugarcane molasses into fructooligosaccharides with enhanced prebiotic activity using whole-cell biocatalysts from Aureobasidium pullulans FRR 5284 and an invertase-deficient Saccharomyces cerevisiae 1403-7A |
| title_fullStr | Transformation of sugarcane molasses into fructooligosaccharides with enhanced prebiotic activity using whole-cell biocatalysts from Aureobasidium pullulans FRR 5284 and an invertase-deficient Saccharomyces cerevisiae 1403-7A |
| title_full_unstemmed | Transformation of sugarcane molasses into fructooligosaccharides with enhanced prebiotic activity using whole-cell biocatalysts from Aureobasidium pullulans FRR 5284 and an invertase-deficient Saccharomyces cerevisiae 1403-7A |
| title_short | Transformation of sugarcane molasses into fructooligosaccharides with enhanced prebiotic activity using whole-cell biocatalysts from Aureobasidium pullulans FRR 5284 and an invertase-deficient Saccharomyces cerevisiae 1403-7A |
| title_sort | transformation of sugarcane molasses into fructooligosaccharides with enhanced prebiotic activity using whole cell biocatalysts from aureobasidium pullulans frr 5284 and an invertase deficient saccharomyces cerevisiae 1403 7a |
| topic | Sugarcane molasses A. pullulans Transfructosylating activity Fructooligosaccharides Prebiotics Probiotics |
| url | https://doi.org/10.1186/s40643-021-00438-7 |
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