Response of MCF-7 Breast Cancer Cells Overexpressed with P-Glycoprotein to Apoptotic Induction after Photodynamic Therapy
Multidrug resistance (MDR) has posed a significant threat to cancer treatment and has led to the emergence of a new therapeutic regime of photodynamic therapy (PDT) to curb the menace. The PDT modality employs a photosensitiser (PS), excited at a specific wavelength of light to kill cancer cells. In...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/23/7412 |
| _version_ | 1827674723404742656 |
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| author | Eric Chekwube Aniogo Blassan P. George Heidi Abrahamse |
| author_facet | Eric Chekwube Aniogo Blassan P. George Heidi Abrahamse |
| author_sort | Eric Chekwube Aniogo |
| collection | DOAJ |
| description | Multidrug resistance (MDR) has posed a significant threat to cancer treatment and has led to the emergence of a new therapeutic regime of photodynamic therapy (PDT) to curb the menace. The PDT modality employs a photosensitiser (PS), excited at a specific wavelength of light to kill cancer cells. In the present study, we used a zinc phthalocyanine tetrasulfonic acid PS to mediate the photodynamic killing of MCF-7 cells overexpressed with P-glycoprotein (P-gp) and investigate the response to cell death induction. After photodynamic treatment, MCF-7 cells undergo cell death, and indicators like Annexin V/PI staining, DNA fragmentation, and measurement of apoptotic protein expression were investigated. Results showed increased externalisation of phosphatidylserine protein, measured as a percentage in flow cytometry indicative of apoptotic induction. This expression was significant (<i>p</i> < 0.006) for the untreated control cells, and there was no detection of DNA fragments after a laser fluence of 20 J/cm<sup>2</sup>. In addition, a statistically significant difference (<i>p</i> < 0.05) was seen in caspase 8 activity and Bax protein expression. These findings were indicative of apoptotic induction and thus seem to represent the extrinsic apoptotic pathway. This study shows the role of PDT in the treatment of a resistant phenotype breast cancer. |
| first_indexed | 2024-03-10T04:47:15Z |
| format | Article |
| id | doaj.art-4c287ad2505345f89dbca8811bc01519 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T04:47:15Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-4c287ad2505345f89dbca8811bc015192023-11-23T02:52:10ZengMDPI AGMolecules1420-30492021-12-012623741210.3390/molecules26237412Response of MCF-7 Breast Cancer Cells Overexpressed with P-Glycoprotein to Apoptotic Induction after Photodynamic TherapyEric Chekwube Aniogo0Blassan P. George1Heidi Abrahamse2Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, South AfricaLaser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, South AfricaLaser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, South AfricaMultidrug resistance (MDR) has posed a significant threat to cancer treatment and has led to the emergence of a new therapeutic regime of photodynamic therapy (PDT) to curb the menace. The PDT modality employs a photosensitiser (PS), excited at a specific wavelength of light to kill cancer cells. In the present study, we used a zinc phthalocyanine tetrasulfonic acid PS to mediate the photodynamic killing of MCF-7 cells overexpressed with P-glycoprotein (P-gp) and investigate the response to cell death induction. After photodynamic treatment, MCF-7 cells undergo cell death, and indicators like Annexin V/PI staining, DNA fragmentation, and measurement of apoptotic protein expression were investigated. Results showed increased externalisation of phosphatidylserine protein, measured as a percentage in flow cytometry indicative of apoptotic induction. This expression was significant (<i>p</i> < 0.006) for the untreated control cells, and there was no detection of DNA fragments after a laser fluence of 20 J/cm<sup>2</sup>. In addition, a statistically significant difference (<i>p</i> < 0.05) was seen in caspase 8 activity and Bax protein expression. These findings were indicative of apoptotic induction and thus seem to represent the extrinsic apoptotic pathway. This study shows the role of PDT in the treatment of a resistant phenotype breast cancer.https://www.mdpi.com/1420-3049/26/23/7412P-glycoproteinmultidrug resistancephotodynamic therapyapoptosis |
| spellingShingle | Eric Chekwube Aniogo Blassan P. George Heidi Abrahamse Response of MCF-7 Breast Cancer Cells Overexpressed with P-Glycoprotein to Apoptotic Induction after Photodynamic Therapy Molecules P-glycoprotein multidrug resistance photodynamic therapy apoptosis |
| title | Response of MCF-7 Breast Cancer Cells Overexpressed with P-Glycoprotein to Apoptotic Induction after Photodynamic Therapy |
| title_full | Response of MCF-7 Breast Cancer Cells Overexpressed with P-Glycoprotein to Apoptotic Induction after Photodynamic Therapy |
| title_fullStr | Response of MCF-7 Breast Cancer Cells Overexpressed with P-Glycoprotein to Apoptotic Induction after Photodynamic Therapy |
| title_full_unstemmed | Response of MCF-7 Breast Cancer Cells Overexpressed with P-Glycoprotein to Apoptotic Induction after Photodynamic Therapy |
| title_short | Response of MCF-7 Breast Cancer Cells Overexpressed with P-Glycoprotein to Apoptotic Induction after Photodynamic Therapy |
| title_sort | response of mcf 7 breast cancer cells overexpressed with p glycoprotein to apoptotic induction after photodynamic therapy |
| topic | P-glycoprotein multidrug resistance photodynamic therapy apoptosis |
| url | https://www.mdpi.com/1420-3049/26/23/7412 |
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