Transcriptome Profiling Reveals Differential Gene Expression during the Process of Microtuber Formation in <i>Pinellia ternata</i>
Using petiole material as explants and directly inducing the formation of microtubers without going through the callus stage is an essential way to rapidly expand scarce medical plants such as <i>Pinellia ternata</i>. However, the early molecular mechanism underlying the formation of the...
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MDPI AG
2023-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/14/11604 |
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| author | Chen Bo Chuandong Su Jingtong Teng Wei Sheng Tao Xue Yanfang Zhu Jianping Xue |
| author_facet | Chen Bo Chuandong Su Jingtong Teng Wei Sheng Tao Xue Yanfang Zhu Jianping Xue |
| author_sort | Chen Bo |
| collection | DOAJ |
| description | Using petiole material as explants and directly inducing the formation of microtubers without going through the callus stage is an essential way to rapidly expand scarce medical plants such as <i>Pinellia ternata</i>. However, the early molecular mechanism underlying the formation of the microtuber is largely elusive. Here, we conducted cytology and dynamic transcriptome analyses of inchoate microtubers in <i>Pinellia</i> explants and identified 1092 differentially expressed genes after their cultivation in vitro for 0, 5, and 15 days. Compared with 0 day, the number and size of the microtuber cells were larger at 5 and 15 days of culture. Detailed categorization revealed that the differentially expressed genes were mainly related to responses to stimulus, biological regulation, organelles, membranes, transcription factor activity, and protein binding. Further analysis revealed that the microtuber at different incubation days exhibited quite a difference in both hormone signaling pathway transduction and the regulation pattern of transcription factors. Therefore, this study contributes to a better understanding of the early molecular regulation during the formation of the microtuber and provides new insights for the study of the rapid expansion of <i>P. ternata</i> and other medical plants. |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T00:59:34Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4c288f1faf324ea19c75d9225efd5fd02023-11-18T19:42:06ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-07-0124141160410.3390/ijms241411604Transcriptome Profiling Reveals Differential Gene Expression during the Process of Microtuber Formation in <i>Pinellia ternata</i>Chen Bo0Chuandong Su1Jingtong Teng2Wei Sheng3Tao Xue4Yanfang Zhu5Jianping Xue6Anhui Provincial Engineering Laboratory for Efficient Utilization of Featured Resource Plants, College of Life Sciences, Huaibei Normal University, Huaibei 235000, ChinaAnhui Provincial Engineering Laboratory for Efficient Utilization of Featured Resource Plants, College of Life Sciences, Huaibei Normal University, Huaibei 235000, ChinaAnhui Provincial Engineering Laboratory for Efficient Utilization of Featured Resource Plants, College of Life Sciences, Huaibei Normal University, Huaibei 235000, ChinaAnhui Provincial Engineering Laboratory for Efficient Utilization of Featured Resource Plants, College of Life Sciences, Huaibei Normal University, Huaibei 235000, ChinaAnhui Provincial Engineering Laboratory for Efficient Utilization of Featured Resource Plants, College of Life Sciences, Huaibei Normal University, Huaibei 235000, ChinaAnhui Provincial Engineering Laboratory for Efficient Utilization of Featured Resource Plants, College of Life Sciences, Huaibei Normal University, Huaibei 235000, ChinaAnhui Provincial Engineering Laboratory for Efficient Utilization of Featured Resource Plants, College of Life Sciences, Huaibei Normal University, Huaibei 235000, ChinaUsing petiole material as explants and directly inducing the formation of microtubers without going through the callus stage is an essential way to rapidly expand scarce medical plants such as <i>Pinellia ternata</i>. However, the early molecular mechanism underlying the formation of the microtuber is largely elusive. Here, we conducted cytology and dynamic transcriptome analyses of inchoate microtubers in <i>Pinellia</i> explants and identified 1092 differentially expressed genes after their cultivation in vitro for 0, 5, and 15 days. Compared with 0 day, the number and size of the microtuber cells were larger at 5 and 15 days of culture. Detailed categorization revealed that the differentially expressed genes were mainly related to responses to stimulus, biological regulation, organelles, membranes, transcription factor activity, and protein binding. Further analysis revealed that the microtuber at different incubation days exhibited quite a difference in both hormone signaling pathway transduction and the regulation pattern of transcription factors. Therefore, this study contributes to a better understanding of the early molecular regulation during the formation of the microtuber and provides new insights for the study of the rapid expansion of <i>P. ternata</i> and other medical plants.https://www.mdpi.com/1422-0067/24/14/11604<i>Pinellia ternata</i>microtuberRNA-seqdifferentially expressed genes (DEGs)expression pattern |
| spellingShingle | Chen Bo Chuandong Su Jingtong Teng Wei Sheng Tao Xue Yanfang Zhu Jianping Xue Transcriptome Profiling Reveals Differential Gene Expression during the Process of Microtuber Formation in <i>Pinellia ternata</i> International Journal of Molecular Sciences <i>Pinellia ternata</i> microtuber RNA-seq differentially expressed genes (DEGs) expression pattern |
| title | Transcriptome Profiling Reveals Differential Gene Expression during the Process of Microtuber Formation in <i>Pinellia ternata</i> |
| title_full | Transcriptome Profiling Reveals Differential Gene Expression during the Process of Microtuber Formation in <i>Pinellia ternata</i> |
| title_fullStr | Transcriptome Profiling Reveals Differential Gene Expression during the Process of Microtuber Formation in <i>Pinellia ternata</i> |
| title_full_unstemmed | Transcriptome Profiling Reveals Differential Gene Expression during the Process of Microtuber Formation in <i>Pinellia ternata</i> |
| title_short | Transcriptome Profiling Reveals Differential Gene Expression during the Process of Microtuber Formation in <i>Pinellia ternata</i> |
| title_sort | transcriptome profiling reveals differential gene expression during the process of microtuber formation in i pinellia ternata i |
| topic | <i>Pinellia ternata</i> microtuber RNA-seq differentially expressed genes (DEGs) expression pattern |
| url | https://www.mdpi.com/1422-0067/24/14/11604 |
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