Characterization and Genome Analysis of Staphylococcus aureus Podovirus CSA13 and Its Anti-Biofilm Capacity
Staphylococcus aureus is one of the notable human pathogens that can be easily encountered in both dietary and clinical surroundings. Among various countermeasures, bacteriophage therapy is recognized as an alternative method for resolving the issue of antibiotic resistance. In the current study, ba...
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MDPI AG
2019-01-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/11/1/54 |
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| author | Yoyeon Cha Jihwan Chun Bokyung Son Sangryeol Ryu |
| author_facet | Yoyeon Cha Jihwan Chun Bokyung Son Sangryeol Ryu |
| author_sort | Yoyeon Cha |
| collection | DOAJ |
| description | Staphylococcus aureus is one of the notable human pathogens that can be easily encountered in both dietary and clinical surroundings. Among various countermeasures, bacteriophage therapy is recognized as an alternative method for resolving the issue of antibiotic resistance. In the current study, bacteriophage CSA13 was isolated from a chicken, and subsequently, its morphology, physiology, and genomics were characterized. This Podoviridae phage displayed an extended host inhibition effect of up to 23 hours of persistence. Its broad host spectrum included methicillin susceptible S. aureus (MSSA), methicillin resistant S. aureus (MRSA), local S. aureus isolates, as well as non-aureus staphylococci strains. Moreover, phage CSA13 could successfully remove over 78% and 93% of MSSA and MRSA biofilms in an experimental setting, respectively. Genomic analysis revealed a 17,034 bp chromosome containing 18 predicted open reading frames (ORFs) without tRNAs, representing a typical chromosomal structure of the staphylococcal Podoviridae family. The results presented here suggest that phage CSA13 can be applicable as an effective biocontrol agent against S. aureus. |
| first_indexed | 2024-12-10T22:03:10Z |
| format | Article |
| id | doaj.art-4c29600df20447a78e901112beb839f2 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-10T22:03:10Z |
| publishDate | 2019-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-4c29600df20447a78e901112beb839f22022-12-22T01:31:51ZengMDPI AGViruses1999-49152019-01-011115410.3390/v11010054v11010054Characterization and Genome Analysis of Staphylococcus aureus Podovirus CSA13 and Its Anti-Biofilm CapacityYoyeon Cha0Jihwan Chun1Bokyung Son2Sangryeol Ryu3Department of Food and Animal Biotechnology, Seoul National University, Seoul 08826, KoreaDepartment of Food and Animal Biotechnology, Seoul National University, Seoul 08826, KoreaDepartment of Food and Animal Biotechnology, Seoul National University, Seoul 08826, KoreaDepartment of Food and Animal Biotechnology, Seoul National University, Seoul 08826, KoreaStaphylococcus aureus is one of the notable human pathogens that can be easily encountered in both dietary and clinical surroundings. Among various countermeasures, bacteriophage therapy is recognized as an alternative method for resolving the issue of antibiotic resistance. In the current study, bacteriophage CSA13 was isolated from a chicken, and subsequently, its morphology, physiology, and genomics were characterized. This Podoviridae phage displayed an extended host inhibition effect of up to 23 hours of persistence. Its broad host spectrum included methicillin susceptible S. aureus (MSSA), methicillin resistant S. aureus (MRSA), local S. aureus isolates, as well as non-aureus staphylococci strains. Moreover, phage CSA13 could successfully remove over 78% and 93% of MSSA and MRSA biofilms in an experimental setting, respectively. Genomic analysis revealed a 17,034 bp chromosome containing 18 predicted open reading frames (ORFs) without tRNAs, representing a typical chromosomal structure of the staphylococcal Podoviridae family. The results presented here suggest that phage CSA13 can be applicable as an effective biocontrol agent against S. aureus.http://www.mdpi.com/1999-4915/11/1/54BacteriophageStaphylococcus aureusanti-biofilmgenome analysis |
| spellingShingle | Yoyeon Cha Jihwan Chun Bokyung Son Sangryeol Ryu Characterization and Genome Analysis of Staphylococcus aureus Podovirus CSA13 and Its Anti-Biofilm Capacity Viruses Bacteriophage Staphylococcus aureus anti-biofilm genome analysis |
| title | Characterization and Genome Analysis of Staphylococcus aureus Podovirus CSA13 and Its Anti-Biofilm Capacity |
| title_full | Characterization and Genome Analysis of Staphylococcus aureus Podovirus CSA13 and Its Anti-Biofilm Capacity |
| title_fullStr | Characterization and Genome Analysis of Staphylococcus aureus Podovirus CSA13 and Its Anti-Biofilm Capacity |
| title_full_unstemmed | Characterization and Genome Analysis of Staphylococcus aureus Podovirus CSA13 and Its Anti-Biofilm Capacity |
| title_short | Characterization and Genome Analysis of Staphylococcus aureus Podovirus CSA13 and Its Anti-Biofilm Capacity |
| title_sort | characterization and genome analysis of staphylococcus aureus podovirus csa13 and its anti biofilm capacity |
| topic | Bacteriophage Staphylococcus aureus anti-biofilm genome analysis |
| url | http://www.mdpi.com/1999-4915/11/1/54 |
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