Morphostatic Speciation within the Dagger Nematode <i>Xiphinema hispanum-</i>Complex Species (Nematoda: Longidoridae)
Dagger nematodes of the genus <i>Xiphinema</i> include a remarkable group of invertebrates of the phylum Nematoda comprising ectoparasitic animals of many wild and cultivated plants. Damage is caused by direct feeding on root cells and by vectoring nepoviruses that cause diseases on seve...
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MDPI AG
2020-11-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/12/1649 |
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| author | Antonio Archidona-Yuste Ruihang Cai Carolina Cantalapiedra-Navarrete José A. Carreira Ana Rey Benjamín Viñegla Gracia Liébanas Juan E. Palomares-Rius Pablo Castillo |
| author_facet | Antonio Archidona-Yuste Ruihang Cai Carolina Cantalapiedra-Navarrete José A. Carreira Ana Rey Benjamín Viñegla Gracia Liébanas Juan E. Palomares-Rius Pablo Castillo |
| author_sort | Antonio Archidona-Yuste |
| collection | DOAJ |
| description | Dagger nematodes of the genus <i>Xiphinema</i> include a remarkable group of invertebrates of the phylum Nematoda comprising ectoparasitic animals of many wild and cultivated plants. Damage is caused by direct feeding on root cells and by vectoring nepoviruses that cause diseases on several crops. Precise identification of <i>Xiphinema</i> species is critical for launching appropriate control measures. We deciphered the cryptic diversity of the <i>Xiphinema hispanum</i>-species complex applying integrative taxonomical approaches that allowed us to verify a paradigmatic example of the morphostatic speciation and the description of a new species, <i>Xiphinema malaka</i> sp. nov. Detailed morphological, morphometrical, multivariate and genetic studies were carried out, and mitochondrial and nuclear haploweb analyses were used for species delimitation of this group. The new species belongs to morphospecies Group 5 from the <i>Xiphinema</i> non<i>americanum</i>-group species. <i>D2-D3</i>, <i>ITS1</i>, partial <i>18S</i>, and partial <i>coxI</i> regions were used for inferring the phylogenetic relationships of <i>X. malaka</i> sp. nov. with other species within the genus <i>Xiphinema</i>. Molecular analyses showed a clear species differentiation not paralleled in morphology and morphometry, reflecting a clear morphostatic speciation. These results support the hypothesis that the biodiversity of dagger nematodes in southern Europe is greater than previously assumed. |
| first_indexed | 2024-03-10T14:32:57Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T14:32:57Z |
| publishDate | 2020-11-01 |
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| series | Plants |
| spelling | doaj.art-4c297d5bf3a64a6c8ed2d6788beb27c82023-11-20T22:23:04ZengMDPI AGPlants2223-77472020-11-01912164910.3390/plants9121649Morphostatic Speciation within the Dagger Nematode <i>Xiphinema hispanum-</i>Complex Species (Nematoda: Longidoridae)Antonio Archidona-Yuste0Ruihang Cai1Carolina Cantalapiedra-Navarrete2José A. Carreira3Ana Rey4Benjamín Viñegla5Gracia Liébanas6Juan E. Palomares-Rius7Pablo Castillo8Department of Ecological Modelling, Helmholtz Centre for Environmental Research—UFZ, Permoserstrasse 15, 04318 Leipzig, GermanyInstituto de Agricultura Sostenible (IAS), Consejo Superior de Investigaciones Científicas (CSIC), Avda. Menéndez Pidal s/n, 14004 Córdoba, SpainInstituto de Agricultura Sostenible (IAS), Consejo Superior de Investigaciones Científicas (CSIC), Avda. Menéndez Pidal s/n, 14004 Córdoba, SpainDepartamento de Biología Animal, Biología Vegetal y Ecología, Universidad de Jaén, Campus Las Lagunillas, 37724 Jaén, SpainDepartamento de Biogeografía y Cambio Global, Museo Nacional de Ciencias Naturales-CSIC, José Abascal 2, 28006 Madrid, SpainDepartamento de Biología Animal, Biología Vegetal y Ecología, Universidad de Jaén, Campus Las Lagunillas, 37724 Jaén, SpainDepartamento de Biología Animal, Biología Vegetal y Ecología, Universidad de Jaén, Campus Las Lagunillas, 37724 Jaén, SpainInstituto de Agricultura Sostenible (IAS), Consejo Superior de Investigaciones Científicas (CSIC), Avda. Menéndez Pidal s/n, 14004 Córdoba, SpainInstituto de Agricultura Sostenible (IAS), Consejo Superior de Investigaciones Científicas (CSIC), Avda. Menéndez Pidal s/n, 14004 Córdoba, SpainDagger nematodes of the genus <i>Xiphinema</i> include a remarkable group of invertebrates of the phylum Nematoda comprising ectoparasitic animals of many wild and cultivated plants. Damage is caused by direct feeding on root cells and by vectoring nepoviruses that cause diseases on several crops. Precise identification of <i>Xiphinema</i> species is critical for launching appropriate control measures. We deciphered the cryptic diversity of the <i>Xiphinema hispanum</i>-species complex applying integrative taxonomical approaches that allowed us to verify a paradigmatic example of the morphostatic speciation and the description of a new species, <i>Xiphinema malaka</i> sp. nov. Detailed morphological, morphometrical, multivariate and genetic studies were carried out, and mitochondrial and nuclear haploweb analyses were used for species delimitation of this group. The new species belongs to morphospecies Group 5 from the <i>Xiphinema</i> non<i>americanum</i>-group species. <i>D2-D3</i>, <i>ITS1</i>, partial <i>18S</i>, and partial <i>coxI</i> regions were used for inferring the phylogenetic relationships of <i>X. malaka</i> sp. nov. with other species within the genus <i>Xiphinema</i>. Molecular analyses showed a clear species differentiation not paralleled in morphology and morphometry, reflecting a clear morphostatic speciation. These results support the hypothesis that the biodiversity of dagger nematodes in southern Europe is greater than previously assumed.https://www.mdpi.com/2223-7747/9/12/1649Bayesian inferencecryptic species<i>coxI</i><i>D2-D3</i> expansion domains of <i>28S</i> rRNA-geneintegrative taxonomyprincipal component analysis |
| spellingShingle | Antonio Archidona-Yuste Ruihang Cai Carolina Cantalapiedra-Navarrete José A. Carreira Ana Rey Benjamín Viñegla Gracia Liébanas Juan E. Palomares-Rius Pablo Castillo Morphostatic Speciation within the Dagger Nematode <i>Xiphinema hispanum-</i>Complex Species (Nematoda: Longidoridae) Plants Bayesian inference cryptic species <i>coxI</i> <i>D2-D3</i> expansion domains of <i>28S</i> rRNA-gene integrative taxonomy principal component analysis |
| title | Morphostatic Speciation within the Dagger Nematode <i>Xiphinema hispanum-</i>Complex Species (Nematoda: Longidoridae) |
| title_full | Morphostatic Speciation within the Dagger Nematode <i>Xiphinema hispanum-</i>Complex Species (Nematoda: Longidoridae) |
| title_fullStr | Morphostatic Speciation within the Dagger Nematode <i>Xiphinema hispanum-</i>Complex Species (Nematoda: Longidoridae) |
| title_full_unstemmed | Morphostatic Speciation within the Dagger Nematode <i>Xiphinema hispanum-</i>Complex Species (Nematoda: Longidoridae) |
| title_short | Morphostatic Speciation within the Dagger Nematode <i>Xiphinema hispanum-</i>Complex Species (Nematoda: Longidoridae) |
| title_sort | morphostatic speciation within the dagger nematode i xiphinema hispanum i complex species nematoda longidoridae |
| topic | Bayesian inference cryptic species <i>coxI</i> <i>D2-D3</i> expansion domains of <i>28S</i> rRNA-gene integrative taxonomy principal component analysis |
| url | https://www.mdpi.com/2223-7747/9/12/1649 |
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