Impact of Dietary or Drinking Water Ruminococcus sp. Supplementation and/or Heat Stress on Growth, Histopathology, and Bursal Gene Expression of Broilers
This research was conducted to evaluate the impact of dietary or drinking water Ruminococcus sp. supplementation and/or heat stress (HS) on the growth, serum biochemistry, tissue antioxidant, phagocytic assay, histopathology, and bursa gene expression of broilers. Day-old broiler chicks were allotte...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-06-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2021.663577/full |
| _version_ | 1797823555334832128 |
|---|---|
| author | Adel Hassan Saad Mohamed S. Ahmed Mohamed Aboubakr Hanan A. Ghoneim Mohamed M. Abdel-Daim Ghadeer M. Albadrani Nagah Arafat Sabreen Ezzat Fadl Walied Abdo |
| author_facet | Adel Hassan Saad Mohamed S. Ahmed Mohamed Aboubakr Hanan A. Ghoneim Mohamed M. Abdel-Daim Ghadeer M. Albadrani Nagah Arafat Sabreen Ezzat Fadl Walied Abdo |
| author_sort | Adel Hassan Saad |
| collection | DOAJ |
| description | This research was conducted to evaluate the impact of dietary or drinking water Ruminococcus sp. supplementation and/or heat stress (HS) on the growth, serum biochemistry, tissue antioxidant, phagocytic assay, histopathology, and bursa gene expression of broilers. Day-old broiler chicks were allotted into six groups according to HS and/or Ruminococcus with or without enzyme supplementation. The first group was the control one, with a formulated diet and normal environmental temperature but without any supplement. The second group fed on Ruminococcus-supplemented diet (1 kg/kg diet). The third group fed on a formulated diet without supplement, and Ruminococcus and digestive enzymes were given in drinking water (0.1 ml/L). The fourth one was the heat stress group, with a normal formulated diet. The fifth and the sixth groups served as second and third groups, respectively, but with heat stress. The results of this experiment indicated that thermal temperature negatively affected the parameters of growth performance, serum biochemical, tissue antioxidants, and phagocytic assay. Moreover, heat stress led to pathological lesions in the internal organs and affected the expression of some genes related to heat stress, including proapoptotic genes such as caspase8 and bax, inflammatory genes such as NF-κβ1, and heat shock protein such as HSP 70 in the bursal tissue. These bad effects and abnormalities were mitigated by Ruminococcus alone or with enzyme supplementation, which improved all the above-mentioned parameters. |
| first_indexed | 2024-03-13T10:25:39Z |
| format | Article |
| id | doaj.art-4c2bdc6c7bac4aadbb90bbc6a7107ffd |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-13T10:25:39Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-4c2bdc6c7bac4aadbb90bbc6a7107ffd2023-05-19T10:30:38ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-06-01810.3389/fvets.2021.663577663577Impact of Dietary or Drinking Water Ruminococcus sp. Supplementation and/or Heat Stress on Growth, Histopathology, and Bursal Gene Expression of BroilersAdel Hassan Saad0Mohamed S. Ahmed1Mohamed Aboubakr2Hanan A. Ghoneim3Mohamed M. Abdel-Daim4Ghadeer M. Albadrani5Nagah Arafat6Sabreen Ezzat Fadl7Walied Abdo8Nutrition and Clinical Nutrition Department, Faculty of Veterinary Medicine, Matrouh University, Mersa Matruh, EgyptPathology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Shaikh, EgyptPharmacology Department, Faculty of Veterinary Medicine, Benha University, Banha, EgyptDepartment of Physiology, Faculty of Veterinary Medicine, Damanhour University, Damanhour, EgyptPharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, EgyptDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi ArabiaDepartment of Poultry Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, EgyptBiochemistry Department, Faculty of Veterinary Medicine, Matrouh University, Mersa Matruh, EgyptPathology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Shaikh, EgyptThis research was conducted to evaluate the impact of dietary or drinking water Ruminococcus sp. supplementation and/or heat stress (HS) on the growth, serum biochemistry, tissue antioxidant, phagocytic assay, histopathology, and bursa gene expression of broilers. Day-old broiler chicks were allotted into six groups according to HS and/or Ruminococcus with or without enzyme supplementation. The first group was the control one, with a formulated diet and normal environmental temperature but without any supplement. The second group fed on Ruminococcus-supplemented diet (1 kg/kg diet). The third group fed on a formulated diet without supplement, and Ruminococcus and digestive enzymes were given in drinking water (0.1 ml/L). The fourth one was the heat stress group, with a normal formulated diet. The fifth and the sixth groups served as second and third groups, respectively, but with heat stress. The results of this experiment indicated that thermal temperature negatively affected the parameters of growth performance, serum biochemical, tissue antioxidants, and phagocytic assay. Moreover, heat stress led to pathological lesions in the internal organs and affected the expression of some genes related to heat stress, including proapoptotic genes such as caspase8 and bax, inflammatory genes such as NF-κβ1, and heat shock protein such as HSP 70 in the bursal tissue. These bad effects and abnormalities were mitigated by Ruminococcus alone or with enzyme supplementation, which improved all the above-mentioned parameters.https://www.frontiersin.org/articles/10.3389/fvets.2021.663577/fullheat stressRuminococcusenzymebiochemistryphagocytic assaypathology |
| spellingShingle | Adel Hassan Saad Mohamed S. Ahmed Mohamed Aboubakr Hanan A. Ghoneim Mohamed M. Abdel-Daim Ghadeer M. Albadrani Nagah Arafat Sabreen Ezzat Fadl Walied Abdo Impact of Dietary or Drinking Water Ruminococcus sp. Supplementation and/or Heat Stress on Growth, Histopathology, and Bursal Gene Expression of Broilers Frontiers in Veterinary Science heat stress Ruminococcus enzyme biochemistry phagocytic assay pathology |
| title | Impact of Dietary or Drinking Water Ruminococcus sp. Supplementation and/or Heat Stress on Growth, Histopathology, and Bursal Gene Expression of Broilers |
| title_full | Impact of Dietary or Drinking Water Ruminococcus sp. Supplementation and/or Heat Stress on Growth, Histopathology, and Bursal Gene Expression of Broilers |
| title_fullStr | Impact of Dietary or Drinking Water Ruminococcus sp. Supplementation and/or Heat Stress on Growth, Histopathology, and Bursal Gene Expression of Broilers |
| title_full_unstemmed | Impact of Dietary or Drinking Water Ruminococcus sp. Supplementation and/or Heat Stress on Growth, Histopathology, and Bursal Gene Expression of Broilers |
| title_short | Impact of Dietary or Drinking Water Ruminococcus sp. Supplementation and/or Heat Stress on Growth, Histopathology, and Bursal Gene Expression of Broilers |
| title_sort | impact of dietary or drinking water ruminococcus sp supplementation and or heat stress on growth histopathology and bursal gene expression of broilers |
| topic | heat stress Ruminococcus enzyme biochemistry phagocytic assay pathology |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2021.663577/full |
| work_keys_str_mv | AT adelhassansaad impactofdietaryordrinkingwaterruminococcusspsupplementationandorheatstressongrowthhistopathologyandbursalgeneexpressionofbroilers AT mohamedsahmed impactofdietaryordrinkingwaterruminococcusspsupplementationandorheatstressongrowthhistopathologyandbursalgeneexpressionofbroilers AT mohamedaboubakr impactofdietaryordrinkingwaterruminococcusspsupplementationandorheatstressongrowthhistopathologyandbursalgeneexpressionofbroilers AT hananaghoneim impactofdietaryordrinkingwaterruminococcusspsupplementationandorheatstressongrowthhistopathologyandbursalgeneexpressionofbroilers AT mohamedmabdeldaim impactofdietaryordrinkingwaterruminococcusspsupplementationandorheatstressongrowthhistopathologyandbursalgeneexpressionofbroilers AT ghadeermalbadrani impactofdietaryordrinkingwaterruminococcusspsupplementationandorheatstressongrowthhistopathologyandbursalgeneexpressionofbroilers AT nagaharafat impactofdietaryordrinkingwaterruminococcusspsupplementationandorheatstressongrowthhistopathologyandbursalgeneexpressionofbroilers AT sabreenezzatfadl impactofdietaryordrinkingwaterruminococcusspsupplementationandorheatstressongrowthhistopathologyandbursalgeneexpressionofbroilers AT waliedabdo impactofdietaryordrinkingwaterruminococcusspsupplementationandorheatstressongrowthhistopathologyandbursalgeneexpressionofbroilers |