Genome-wide identification and characterisation of ammonium transporter gene family in barley
Nitrogen (N) is an essential macronutrient for the growth and development of plants, but excessive use of nitrogen fertiliser in agriculture can result in environmental pollution. As a preferred nitrogen form, ammonium (NH 4 +) is absorbed from the soil by the plants through ammonium transporters (A...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Polish Academy of Sciences
2023-09-01
|
| Series: | Journal of Water and Land Development |
| Subjects: | |
| Online Access: | https://journals.pan.pl/Content/128744/PDF/2023-03-JWLD-24.pdf |
| _version_ | 1797659242971267072 |
|---|---|
| author | Umesh K. Tanwar Ewelina Stolarska Ewelina Paluch-Lubawa Elżbieta Rudy Ewa Sobieszczuk-Nowicka |
| author_facet | Umesh K. Tanwar Ewelina Stolarska Ewelina Paluch-Lubawa Elżbieta Rudy Ewa Sobieszczuk-Nowicka |
| author_sort | Umesh K. Tanwar |
| collection | DOAJ |
| description | Nitrogen (N) is an essential macronutrient for the growth and development of plants, but excessive use of nitrogen fertiliser in agriculture can result in environmental pollution. As a preferred nitrogen form, ammonium (NH 4 +) is absorbed from the soil by the plants through ammonium transporters (AMTs). Therefore, it is important to explore AMTs to improve the efficiency of plant N utilisation. Here, we performed a comprehensive genome-wide analysis to identify and characterise the AMT genes in barley ( HvAMTs), which is a very important cereal crop. A total of seven AMT genes were identified in barley and further divided into two subfamilies ( AMT1 and AMT2) based on phylogenetic analysis. All HvAMT genes were distributed on five chromosomes with only one tandem duplication. HvAMTs might play an important role in plant growth, development, and various stress responses, as indicated by cis- regulatory elements, miRNAs, and protein interaction analysis. Further, we analysed the expression pattern of HvAMTs in various developmental plant tissues, which indicated that AMT1 subfamily members might play a major role in the uptake of NH 4 + from the soil through the roots in barley. Altogether, these findings might be helpful to improve the barley crop with improved nitrogen use efficiency, which is not only of great significance to the crop but also for land and water as it will reduce N fertiliser pollution in the surrounding ecosystem. |
| first_indexed | 2024-03-11T18:12:01Z |
| format | Article |
| id | doaj.art-4c2d8b3889b54e9cb64ef327cc7a76ca |
| institution | Directory Open Access Journal |
| issn | 2083-4535 |
| language | English |
| last_indexed | 2024-03-11T18:12:01Z |
| publishDate | 2023-09-01 |
| publisher | Polish Academy of Sciences |
| record_format | Article |
| series | Journal of Water and Land Development |
| spelling | doaj.art-4c2d8b3889b54e9cb64ef327cc7a76ca2023-10-16T12:12:37ZengPolish Academy of SciencesJournal of Water and Land Development2083-45352023-09-01No 58220233https://doi.org/10.24425/jwld.2023.146614Genome-wide identification and characterisation of ammonium transporter gene family in barleyUmesh K. Tanwar0https://orcid.org/0000-0003-1032-6816Ewelina Stolarska1https://orcid.org/0000-0002-1774-3762Ewelina Paluch-Lubawa2https://orcid.org/0000-0003-4456-0758Elżbieta Rudy3https://orcid.org/0000-0003-3548-3500Ewa Sobieszczuk-Nowicka4https://orcid.org/0000-0002-6603-0582Adam Mickiewicz University, Faculty of Biology, Department of Plant Physiology, ul. Uniwersytetu Poznańskiego 6, 61-614, Poznań, PolandAdam Mickiewicz University, Faculty of Biology, Department of Plant Physiology, ul. Uniwersytetu Poznańskiego 6, 61-614, Poznań, PolandAdam Mickiewicz University, Faculty of Biology, Department of Plant Physiology, ul. Uniwersytetu Poznańskiego 6, 61-614, Poznań, PolandAdam Mickiewicz University, Faculty of Biology, Department of Plant Physiology, ul. Uniwersytetu Poznańskiego 6, 61-614, Poznań, PolandAdam Mickiewicz University, Faculty of Biology, Department of Plant Physiology, ul. Uniwersytetu Poznańskiego 6, 61-614, Poznań, PolandNitrogen (N) is an essential macronutrient for the growth and development of plants, but excessive use of nitrogen fertiliser in agriculture can result in environmental pollution. As a preferred nitrogen form, ammonium (NH 4 +) is absorbed from the soil by the plants through ammonium transporters (AMTs). Therefore, it is important to explore AMTs to improve the efficiency of plant N utilisation. Here, we performed a comprehensive genome-wide analysis to identify and characterise the AMT genes in barley ( HvAMTs), which is a very important cereal crop. A total of seven AMT genes were identified in barley and further divided into two subfamilies ( AMT1 and AMT2) based on phylogenetic analysis. All HvAMT genes were distributed on five chromosomes with only one tandem duplication. HvAMTs might play an important role in plant growth, development, and various stress responses, as indicated by cis- regulatory elements, miRNAs, and protein interaction analysis. Further, we analysed the expression pattern of HvAMTs in various developmental plant tissues, which indicated that AMT1 subfamily members might play a major role in the uptake of NH 4 + from the soil through the roots in barley. Altogether, these findings might be helpful to improve the barley crop with improved nitrogen use efficiency, which is not only of great significance to the crop but also for land and water as it will reduce N fertiliser pollution in the surrounding ecosystem.https://journals.pan.pl/Content/128744/PDF/2023-03-JWLD-24.pdfammonium transporter (amt)bioinformaticsgene expressionbarley (hordeum vulgare)itrogen use efficiency |
| spellingShingle | Umesh K. Tanwar Ewelina Stolarska Ewelina Paluch-Lubawa Elżbieta Rudy Ewa Sobieszczuk-Nowicka Genome-wide identification and characterisation of ammonium transporter gene family in barley Journal of Water and Land Development ammonium transporter (amt) bioinformatics gene expression barley (hordeum vulgare) itrogen use efficiency |
| title | Genome-wide identification and characterisation of ammonium transporter gene family in barley |
| title_full | Genome-wide identification and characterisation of ammonium transporter gene family in barley |
| title_fullStr | Genome-wide identification and characterisation of ammonium transporter gene family in barley |
| title_full_unstemmed | Genome-wide identification and characterisation of ammonium transporter gene family in barley |
| title_short | Genome-wide identification and characterisation of ammonium transporter gene family in barley |
| title_sort | genome wide identification and characterisation of ammonium transporter gene family in barley |
| topic | ammonium transporter (amt) bioinformatics gene expression barley (hordeum vulgare) itrogen use efficiency |
| url | https://journals.pan.pl/Content/128744/PDF/2023-03-JWLD-24.pdf |
| work_keys_str_mv | AT umeshktanwar genomewideidentificationandcharacterisationofammoniumtransportergenefamilyinbarley AT ewelinastolarska genomewideidentificationandcharacterisationofammoniumtransportergenefamilyinbarley AT ewelinapaluchlubawa genomewideidentificationandcharacterisationofammoniumtransportergenefamilyinbarley AT elzbietarudy genomewideidentificationandcharacterisationofammoniumtransportergenefamilyinbarley AT ewasobieszczuknowicka genomewideidentificationandcharacterisationofammoniumtransportergenefamilyinbarley |