Nephroprotective effect of Physalis peruviana L. calyx extract and its butanolic fraction against cadmium chloride toxicity in rats and molecular docking of isolated compounds
Abstract Background Cadmium is an environmentally toxic metal that has deleterious effects on both animals and humans due to its accumulation in different body tissues. Physalis peruviana L. fruit and calyx contain many active constituents which are used traditionally for their different biological...
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| Format: | Article |
| Language: | English |
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BMC
2023-01-01
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| Series: | BMC Complementary Medicine and Therapies |
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| Online Access: | https://doi.org/10.1186/s12906-023-03845-9 |
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| author | Hesham S. M. Soliman Eslam M. Korany Elsayed K. El-Sayed Ahmed M. Aboelyazed Haitham A. Ibrahim |
| author_facet | Hesham S. M. Soliman Eslam M. Korany Elsayed K. El-Sayed Ahmed M. Aboelyazed Haitham A. Ibrahim |
| author_sort | Hesham S. M. Soliman |
| collection | DOAJ |
| description | Abstract Background Cadmium is an environmentally toxic metal that has deleterious effects on both animals and humans due to its accumulation in different body tissues. Physalis peruviana L. fruit and calyx contain many active constituents which are used traditionally for their different biological activities. Based on the traditional uses of P. peruviana L. calyx, we aimed to evaluate the nephroprotective effect of their 80% aqueous methanol extract (AME) and n-butanol fraction (Bu.F.) against cadmium chloride-induced nephrotoxicity in rats and to correlate this activity with phytoconstituents isolated using molecular docking studies. Methods The n-butanol fraction of P. peruviana L. calyx was fractionated using various chromatographic techniques and the isolated compounds were identified based on their chemical and spectroscopic data. The nephroprotective activity was assessed using cadmium chloride-induced nephrotoxicity in the rat model, by measuring some important parameters such as body weight, kidney weight, serum urea, and creatinine levels, oxidative stress markers, inflammatory markers, and histopathological examinations of kidney tissue. Molecular docking studies of the isolated compounds were performed. Results Three withanolides named 4 β-hydroxywithanolide E (1), Physalin B (2) and 3α, 14β-dihydroxywithaphysalin N (3) were isolated and identified from the n-butanol fraction of P. peruviana L calyx extract. The extract and its butanol fraction significantly improved the serum kidney function markers and tissue oxidative status including malondialdehyde (MDA), reduced glutathione (GSH) and catalase (CAT). Additionally, the extracts significantly decreased the levels of tumor necrosis factor-alpha (TNF-α) and nuclear factor kappaB (NF-κβ). Moreover, the histological changes were ameliorated by the extracts. The molecular docking study showed that the isolated compounds displayed a remarkable inhibitory activity against IκB kinase. Conclusion The AME and its butanol fraction of P. peruviana L calyx showed potential nephroprotective activity against cadmium chloride-induced nephrotoxicity which is correlated at least in part to its considerable withanolides content. |
| first_indexed | 2024-04-10T19:45:06Z |
| format | Article |
| id | doaj.art-4c3015f491584795be77d7b2291dfe2b |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-04-10T19:45:06Z |
| publishDate | 2023-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-4c3015f491584795be77d7b2291dfe2b2023-01-29T12:04:15ZengBMCBMC Complementary Medicine and Therapies2662-76712023-01-0123111510.1186/s12906-023-03845-9Nephroprotective effect of Physalis peruviana L. calyx extract and its butanolic fraction against cadmium chloride toxicity in rats and molecular docking of isolated compoundsHesham S. M. Soliman0Eslam M. Korany1Elsayed K. El-Sayed2Ahmed M. Aboelyazed3Haitham A. Ibrahim4Department of Pharmacognosy, Faculty of Pharmacy, Helwan UniversityDepartment of Pharmacognosy, Faculty of Pharmacy, Helwan UniversityDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Helwan UniversityDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Badr UniversityDepartment of Pharmacognosy, Faculty of Pharmacy, Helwan UniversityAbstract Background Cadmium is an environmentally toxic metal that has deleterious effects on both animals and humans due to its accumulation in different body tissues. Physalis peruviana L. fruit and calyx contain many active constituents which are used traditionally for their different biological activities. Based on the traditional uses of P. peruviana L. calyx, we aimed to evaluate the nephroprotective effect of their 80% aqueous methanol extract (AME) and n-butanol fraction (Bu.F.) against cadmium chloride-induced nephrotoxicity in rats and to correlate this activity with phytoconstituents isolated using molecular docking studies. Methods The n-butanol fraction of P. peruviana L. calyx was fractionated using various chromatographic techniques and the isolated compounds were identified based on their chemical and spectroscopic data. The nephroprotective activity was assessed using cadmium chloride-induced nephrotoxicity in the rat model, by measuring some important parameters such as body weight, kidney weight, serum urea, and creatinine levels, oxidative stress markers, inflammatory markers, and histopathological examinations of kidney tissue. Molecular docking studies of the isolated compounds were performed. Results Three withanolides named 4 β-hydroxywithanolide E (1), Physalin B (2) and 3α, 14β-dihydroxywithaphysalin N (3) were isolated and identified from the n-butanol fraction of P. peruviana L calyx extract. The extract and its butanol fraction significantly improved the serum kidney function markers and tissue oxidative status including malondialdehyde (MDA), reduced glutathione (GSH) and catalase (CAT). Additionally, the extracts significantly decreased the levels of tumor necrosis factor-alpha (TNF-α) and nuclear factor kappaB (NF-κβ). Moreover, the histological changes were ameliorated by the extracts. The molecular docking study showed that the isolated compounds displayed a remarkable inhibitory activity against IκB kinase. Conclusion The AME and its butanol fraction of P. peruviana L calyx showed potential nephroprotective activity against cadmium chloride-induced nephrotoxicity which is correlated at least in part to its considerable withanolides content.https://doi.org/10.1186/s12906-023-03845-9Anti-inflammatoryAntioxidantNephrotoxicityPhysalis peruviana L calyxWithanolides |
| spellingShingle | Hesham S. M. Soliman Eslam M. Korany Elsayed K. El-Sayed Ahmed M. Aboelyazed Haitham A. Ibrahim Nephroprotective effect of Physalis peruviana L. calyx extract and its butanolic fraction against cadmium chloride toxicity in rats and molecular docking of isolated compounds BMC Complementary Medicine and Therapies Anti-inflammatory Antioxidant Nephrotoxicity Physalis peruviana L calyx Withanolides |
| title | Nephroprotective effect of Physalis peruviana L. calyx extract and its butanolic fraction against cadmium chloride toxicity in rats and molecular docking of isolated compounds |
| title_full | Nephroprotective effect of Physalis peruviana L. calyx extract and its butanolic fraction against cadmium chloride toxicity in rats and molecular docking of isolated compounds |
| title_fullStr | Nephroprotective effect of Physalis peruviana L. calyx extract and its butanolic fraction against cadmium chloride toxicity in rats and molecular docking of isolated compounds |
| title_full_unstemmed | Nephroprotective effect of Physalis peruviana L. calyx extract and its butanolic fraction against cadmium chloride toxicity in rats and molecular docking of isolated compounds |
| title_short | Nephroprotective effect of Physalis peruviana L. calyx extract and its butanolic fraction against cadmium chloride toxicity in rats and molecular docking of isolated compounds |
| title_sort | nephroprotective effect of physalis peruviana l calyx extract and its butanolic fraction against cadmium chloride toxicity in rats and molecular docking of isolated compounds |
| topic | Anti-inflammatory Antioxidant Nephrotoxicity Physalis peruviana L calyx Withanolides |
| url | https://doi.org/10.1186/s12906-023-03845-9 |
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