Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids.
To detect and study diseases, research and clinical laboratories must quantify specific biomarkers in the plasma and urine of patients with precision, sensitivity, and cost-effectiveness. Newly developed techniques, such as particle-based immunoassays, must be validated in these terms against standa...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC6054379?pdf=render |
| _version_ | 1828191725939589120 |
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| author | Sujit Kumar Verma Anja Karin Albrecht Verena Siebecke Gerd Klöck Tatiana A Kolesnikova Sebastian Springer |
| author_facet | Sujit Kumar Verma Anja Karin Albrecht Verena Siebecke Gerd Klöck Tatiana A Kolesnikova Sebastian Springer |
| author_sort | Sujit Kumar Verma |
| collection | DOAJ |
| description | To detect and study diseases, research and clinical laboratories must quantify specific biomarkers in the plasma and urine of patients with precision, sensitivity, and cost-effectiveness. Newly developed techniques, such as particle-based immunoassays, must be validated in these terms against standard methods such as enzyme-linked immunosorbent assays (ELISAs). Here, we compare the performance of assays that use hollow polyelectrolyte microcapsules with assays based on solid plastic beads, and with standard microplate immunoassays. The polyelectrolyte microcapsules detect the disease biomarker beta-2 microglobulin with a fifty-fold increase in sensitivity than polystyrene (PS) beads. For sequence-specific nucleic acid detection, the oligonucleotide-coated microcapsules exhibit a two-fold lower increase in sensitivity over PS beads. The microcapsules also detect the presence of a monoclonal antibody in hybridoma supernatant at a fifty-six-fold increase in sensitivity compared to a microplate assay. Overall, polyelectrolyte microcapsule-based assays are more sensitive for the detection of protein and nucleic acid analytes than PS beads and microplate assays, and they are viable alternatives as a platform for the rapid quantitative detection of analytes at very low concentrations. |
| first_indexed | 2024-04-12T08:43:46Z |
| format | Article |
| id | doaj.art-4c33fd789c5d4f278a9ac60bf3926566 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T08:43:46Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4c33fd789c5d4f278a9ac60bf39265662022-12-22T03:39:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01137e020100910.1371/journal.pone.0201009Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids.Sujit Kumar VermaAnja Karin AlbrechtVerena SiebeckeGerd KlöckTatiana A KolesnikovaSebastian SpringerTo detect and study diseases, research and clinical laboratories must quantify specific biomarkers in the plasma and urine of patients with precision, sensitivity, and cost-effectiveness. Newly developed techniques, such as particle-based immunoassays, must be validated in these terms against standard methods such as enzyme-linked immunosorbent assays (ELISAs). Here, we compare the performance of assays that use hollow polyelectrolyte microcapsules with assays based on solid plastic beads, and with standard microplate immunoassays. The polyelectrolyte microcapsules detect the disease biomarker beta-2 microglobulin with a fifty-fold increase in sensitivity than polystyrene (PS) beads. For sequence-specific nucleic acid detection, the oligonucleotide-coated microcapsules exhibit a two-fold lower increase in sensitivity over PS beads. The microcapsules also detect the presence of a monoclonal antibody in hybridoma supernatant at a fifty-six-fold increase in sensitivity compared to a microplate assay. Overall, polyelectrolyte microcapsule-based assays are more sensitive for the detection of protein and nucleic acid analytes than PS beads and microplate assays, and they are viable alternatives as a platform for the rapid quantitative detection of analytes at very low concentrations.http://europepmc.org/articles/PMC6054379?pdf=render |
| spellingShingle | Sujit Kumar Verma Anja Karin Albrecht Verena Siebecke Gerd Klöck Tatiana A Kolesnikova Sebastian Springer Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids. PLoS ONE |
| title | Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids. |
| title_full | Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids. |
| title_fullStr | Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids. |
| title_full_unstemmed | Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids. |
| title_short | Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids. |
| title_sort | comparative validation of a microcapsule based immunoassay for the detection of proteins and nucleic acids |
| url | http://europepmc.org/articles/PMC6054379?pdf=render |
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