Heterologous Expression and Enzymatic Properties of Nicotinamide Nucleoside Kinase from Saccharomyces cerevisiae
In this study, the nicotinamide riboside kinase 1 gene (ScNRK1) was cloned from Saccharomyces cerevisiae, and its soluble expression in Escherichia coli was achieved using pET28a plasmid. The enzyme activity in the fermentation broth was 14.75 IU/mL, and the specific activity the purified enzyme was...
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| Format: | Article |
| Language: | English |
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China Food Publishing Company
2023-08-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-16-024.pdf |
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| author | HE Jianju, LIU Xinxin, SHI Hongling, RAN Luni, WANG Xian, ZHANG Yingjun, TANG Cunduo |
| author_facet | HE Jianju, LIU Xinxin, SHI Hongling, RAN Luni, WANG Xian, ZHANG Yingjun, TANG Cunduo |
| author_sort | HE Jianju, LIU Xinxin, SHI Hongling, RAN Luni, WANG Xian, ZHANG Yingjun, TANG Cunduo |
| collection | DOAJ |
| description | In this study, the nicotinamide riboside kinase 1 gene (ScNRK1) was cloned from Saccharomyces cerevisiae, and its soluble expression in Escherichia coli was achieved using pET28a plasmid. The enzyme activity in the fermentation broth was 14.75 IU/mL, and the specific activity the purified enzyme was 2 252.59 IU/mg. In addition, the kinetic parameters of ScNRK1 were significantly higher than those of other reported nicotinamide nucleoside kinases, so ScNRK1 could be more advantageous in the enzymatic synthesis of nicotinamide mononucleotide (NMN). |
| first_indexed | 2024-03-12T00:40:11Z |
| format | Article |
| id | doaj.art-4c37ab565d4c4eca8595f8483e402ec8 |
| institution | Directory Open Access Journal |
| issn | 1002-6630 |
| language | English |
| last_indexed | 2024-03-12T00:40:11Z |
| publishDate | 2023-08-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj.art-4c37ab565d4c4eca8595f8483e402ec82023-09-15T07:43:35ZengChina Food Publishing CompanyShipin Kexue1002-66302023-08-01441619219710.7506/spkx1002-6630-20220912-095Heterologous Expression and Enzymatic Properties of Nicotinamide Nucleoside Kinase from Saccharomyces cerevisiaeHE Jianju, LIU Xinxin, SHI Hongling, RAN Luni, WANG Xian, ZHANG Yingjun, TANG Cunduo0(1. College of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China; 2. Postdoctoral Innovation Practice Base, She Dian Lao Jiu Co. Ltd., Nanyang 473300, China; 3. College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China)In this study, the nicotinamide riboside kinase 1 gene (ScNRK1) was cloned from Saccharomyces cerevisiae, and its soluble expression in Escherichia coli was achieved using pET28a plasmid. The enzyme activity in the fermentation broth was 14.75 IU/mL, and the specific activity the purified enzyme was 2 252.59 IU/mg. In addition, the kinetic parameters of ScNRK1 were significantly higher than those of other reported nicotinamide nucleoside kinases, so ScNRK1 could be more advantageous in the enzymatic synthesis of nicotinamide mononucleotide (NMN).https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-16-024.pdfnicotinamide nucleoside kinase; β-nicotinamide mononucleotide; expression; identification; biocatalysis |
| spellingShingle | HE Jianju, LIU Xinxin, SHI Hongling, RAN Luni, WANG Xian, ZHANG Yingjun, TANG Cunduo Heterologous Expression and Enzymatic Properties of Nicotinamide Nucleoside Kinase from Saccharomyces cerevisiae Shipin Kexue nicotinamide nucleoside kinase; β-nicotinamide mononucleotide; expression; identification; biocatalysis |
| title | Heterologous Expression and Enzymatic Properties of Nicotinamide Nucleoside Kinase from Saccharomyces cerevisiae |
| title_full | Heterologous Expression and Enzymatic Properties of Nicotinamide Nucleoside Kinase from Saccharomyces cerevisiae |
| title_fullStr | Heterologous Expression and Enzymatic Properties of Nicotinamide Nucleoside Kinase from Saccharomyces cerevisiae |
| title_full_unstemmed | Heterologous Expression and Enzymatic Properties of Nicotinamide Nucleoside Kinase from Saccharomyces cerevisiae |
| title_short | Heterologous Expression and Enzymatic Properties of Nicotinamide Nucleoside Kinase from Saccharomyces cerevisiae |
| title_sort | heterologous expression and enzymatic properties of nicotinamide nucleoside kinase from saccharomyces cerevisiae |
| topic | nicotinamide nucleoside kinase; β-nicotinamide mononucleotide; expression; identification; biocatalysis |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-16-024.pdf |
| work_keys_str_mv | AT hejianjuliuxinxinshihonglingranluniwangxianzhangyingjuntangcunduo heterologousexpressionandenzymaticpropertiesofnicotinamidenucleosidekinasefromsaccharomycescerevisiae |