Proteomic Analysis of Resistance Mediated by Rcm 2.0 and Rcm 5.1, Two Loci Controlling Resistance to Bacterial Canker of Tomato
Two quantitative trait loci from Lycopersicon hirsutum, Rcm 2.0 and Rcm 5.1, control resistance to Clavibacter michiganensis subsp. michiganensis, the causal agent of bacterial canker of tomato. Lines containing Rcm 2.0 and Rcm 5.1 and a susceptible control line were compared at 72 and 144 h postino...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2004-09-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2004.17.9.1019 |
| _version_ | 1811291254366928896 |
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| author | Gitta L. Coaker Belinda Willard Michael Kinter Eric J. Stockinger David M. Francis |
| author_facet | Gitta L. Coaker Belinda Willard Michael Kinter Eric J. Stockinger David M. Francis |
| author_sort | Gitta L. Coaker |
| collection | DOAJ |
| description | Two quantitative trait loci from Lycopersicon hirsutum, Rcm 2.0 and Rcm 5.1, control resistance to Clavibacter michiganensis subsp. michiganensis, the causal agent of bacterial canker of tomato. Lines containing Rcm 2.0 and Rcm 5.1 and a susceptible control line were compared at 72 and 144 h postinoculation, using 2-dimensional gel electrophoresis to identify proteins regulated in response to C. michiganensis subsp. michiganensis infection. A total of 47 proteins were subjected to tandem mass spectrometry. Database queries with resulting spectra identified tomato genes for 26 proteins. The remaining 21 proteins were either identified in other species or possessed no homology to known proteins. Spectra were interpreted to deduce peptide amino acid sequences that were then used to query publicly available data. This approach identified tomato genes or expressed sequence tags for 44 of the proteins analyzed. Three superoxide dismutase (SOD) enzymes were differentially regulated among genotypes, and patterns of hydrogen peroxide accumulation were genotype- and tissue-specific, indicating a role for oxidative stress in response to C. michiganensis subsp. michiganensis. Steady-state mRNA and protein levels for SOD, thioredoxin M-type, S-adenosylhomocysteine hydrolase, and pathogenesis-related proteins demonstrated similar patterns of differential regulation. Lines containing Rcm 2.0 and Rcm 5.1 accumulate different proteins and steady-state mRNAs in response to inoculation, suggesting that the two loci may confer resistance through distinct mechanisms. |
| first_indexed | 2024-04-13T04:26:43Z |
| format | Article |
| id | doaj.art-4c39d76f7cb245978d415c7404e960de |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:26:43Z |
| publishDate | 2004-09-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-4c39d76f7cb245978d415c7404e960de2022-12-22T03:02:28ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062004-09-011791019102810.1094/MPMI.2004.17.9.1019Proteomic Analysis of Resistance Mediated by Rcm 2.0 and Rcm 5.1, Two Loci Controlling Resistance to Bacterial Canker of TomatoGitta L. CoakerBelinda WillardMichael KinterEric J. StockingerDavid M. FrancisTwo quantitative trait loci from Lycopersicon hirsutum, Rcm 2.0 and Rcm 5.1, control resistance to Clavibacter michiganensis subsp. michiganensis, the causal agent of bacterial canker of tomato. Lines containing Rcm 2.0 and Rcm 5.1 and a susceptible control line were compared at 72 and 144 h postinoculation, using 2-dimensional gel electrophoresis to identify proteins regulated in response to C. michiganensis subsp. michiganensis infection. A total of 47 proteins were subjected to tandem mass spectrometry. Database queries with resulting spectra identified tomato genes for 26 proteins. The remaining 21 proteins were either identified in other species or possessed no homology to known proteins. Spectra were interpreted to deduce peptide amino acid sequences that were then used to query publicly available data. This approach identified tomato genes or expressed sequence tags for 44 of the proteins analyzed. Three superoxide dismutase (SOD) enzymes were differentially regulated among genotypes, and patterns of hydrogen peroxide accumulation were genotype- and tissue-specific, indicating a role for oxidative stress in response to C. michiganensis subsp. michiganensis. Steady-state mRNA and protein levels for SOD, thioredoxin M-type, S-adenosylhomocysteine hydrolase, and pathogenesis-related proteins demonstrated similar patterns of differential regulation. Lines containing Rcm 2.0 and Rcm 5.1 accumulate different proteins and steady-state mRNAs in response to inoculation, suggesting that the two loci may confer resistance through distinct mechanisms.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2004.17.9.10192-DEgram-positivedisease resistancequantitative resistanceESI-MS/MS |
| spellingShingle | Gitta L. Coaker Belinda Willard Michael Kinter Eric J. Stockinger David M. Francis Proteomic Analysis of Resistance Mediated by Rcm 2.0 and Rcm 5.1, Two Loci Controlling Resistance to Bacterial Canker of Tomato Molecular Plant-Microbe Interactions 2-DE gram-positive disease resistance quantitative resistance ESI-MS/MS |
| title | Proteomic Analysis of Resistance Mediated by Rcm 2.0 and Rcm 5.1, Two Loci Controlling Resistance to Bacterial Canker of Tomato |
| title_full | Proteomic Analysis of Resistance Mediated by Rcm 2.0 and Rcm 5.1, Two Loci Controlling Resistance to Bacterial Canker of Tomato |
| title_fullStr | Proteomic Analysis of Resistance Mediated by Rcm 2.0 and Rcm 5.1, Two Loci Controlling Resistance to Bacterial Canker of Tomato |
| title_full_unstemmed | Proteomic Analysis of Resistance Mediated by Rcm 2.0 and Rcm 5.1, Two Loci Controlling Resistance to Bacterial Canker of Tomato |
| title_short | Proteomic Analysis of Resistance Mediated by Rcm 2.0 and Rcm 5.1, Two Loci Controlling Resistance to Bacterial Canker of Tomato |
| title_sort | proteomic analysis of resistance mediated by rcm 2 0 and rcm 5 1 two loci controlling resistance to bacterial canker of tomato |
| topic | 2-DE gram-positive disease resistance quantitative resistance ESI-MS/MS |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2004.17.9.1019 |
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