Effect of Hesperetin on the level of reactive oxygen species (ROS) in gastric cancer stem cells: Short Communication
Intracellular reactive oxygen species (ROS) play an important role in cancer stem cell (CSC) function. Hesperetin (Hst) is a flavonoid that has been shown to affect cellular ROS level. The goal of this study was to investigate the effect of Hst on the level of ROS in gastric CSCs (GCSCs). MTT assay...
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| Format: | Article |
| Language: | fas |
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Birjand University of Medical Sciences and Health Services
2022-04-01
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| Series: | مجله دانشگاه علوم پزشکی بیرجند |
| Subjects: | |
| Online Access: | http://journal.bums.ac.ir/article-1-2997-en.html |
| _version_ | 1817969854651564032 |
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| author | Vahid Bagheri Mohammad Zangooei |
| author_facet | Vahid Bagheri Mohammad Zangooei |
| author_sort | Vahid Bagheri |
| collection | DOAJ |
| description | Intracellular reactive oxygen species (ROS) play an important role in cancer stem cell (CSC) function. Hesperetin (Hst) is a flavonoid that has been shown to affect cellular ROS level. The goal of this study was to investigate the effect of Hst on the level of ROS in gastric CSCs (GCSCs). MTT assay was used to evaluate cell survival. Cellular ROS level was measured using 2′,7′-dichlorofluorescin diacetate by flow cytometry. A significant decrease in the survival of GCSCs treated with Hst was observed. Hst decreased ROS level to about 47% and 40% at concentrations of 200 and 400 mM, respectively. It can be concluded that Hst reduces the survival and ROS level of GCSCs and may be influences the function of these cells such as proliferation and differentiation as well as response to chemotherapy by affecting cellular ROS level. |
| first_indexed | 2024-04-13T20:26:30Z |
| format | Article |
| id | doaj.art-4c406984c4034797b6dae9377be916f4 |
| institution | Directory Open Access Journal |
| issn | 1607-2197 2423-6152 |
| language | fas |
| last_indexed | 2024-04-13T20:26:30Z |
| publishDate | 2022-04-01 |
| publisher | Birjand University of Medical Sciences and Health Services |
| record_format | Article |
| series | مجله دانشگاه علوم پزشکی بیرجند |
| spelling | doaj.art-4c406984c4034797b6dae9377be916f42022-12-22T02:31:19ZfasBirjand University of Medical Sciences and Health Servicesمجله دانشگاه علوم پزشکی بیرجند1607-21972423-61522022-04-012915056Effect of Hesperetin on the level of reactive oxygen species (ROS) in gastric cancer stem cells: Short CommunicationVahid Bagheri0Mohammad Zangooei1 Cellular and Molecular Research Center, Department of Medical Immunology, Birjand University of Medical Sciences, Birjand, Iran Department of Biochemistry, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran Intracellular reactive oxygen species (ROS) play an important role in cancer stem cell (CSC) function. Hesperetin (Hst) is a flavonoid that has been shown to affect cellular ROS level. The goal of this study was to investigate the effect of Hst on the level of ROS in gastric CSCs (GCSCs). MTT assay was used to evaluate cell survival. Cellular ROS level was measured using 2′,7′-dichlorofluorescin diacetate by flow cytometry. A significant decrease in the survival of GCSCs treated with Hst was observed. Hst decreased ROS level to about 47% and 40% at concentrations of 200 and 400 mM, respectively. It can be concluded that Hst reduces the survival and ROS level of GCSCs and may be influences the function of these cells such as proliferation and differentiation as well as response to chemotherapy by affecting cellular ROS level.http://journal.bums.ac.ir/article-1-2997-en.htmlcancer stem cellcell survivalhesperetinreactive oxygen species. |
| spellingShingle | Vahid Bagheri Mohammad Zangooei Effect of Hesperetin on the level of reactive oxygen species (ROS) in gastric cancer stem cells: Short Communication مجله دانشگاه علوم پزشکی بیرجند cancer stem cell cell survival hesperetin reactive oxygen species. |
| title | Effect of Hesperetin on the level of reactive oxygen species (ROS) in gastric cancer stem cells: Short Communication |
| title_full | Effect of Hesperetin on the level of reactive oxygen species (ROS) in gastric cancer stem cells: Short Communication |
| title_fullStr | Effect of Hesperetin on the level of reactive oxygen species (ROS) in gastric cancer stem cells: Short Communication |
| title_full_unstemmed | Effect of Hesperetin on the level of reactive oxygen species (ROS) in gastric cancer stem cells: Short Communication |
| title_short | Effect of Hesperetin on the level of reactive oxygen species (ROS) in gastric cancer stem cells: Short Communication |
| title_sort | effect of hesperetin on the level of reactive oxygen species ros in gastric cancer stem cells short communication |
| topic | cancer stem cell cell survival hesperetin reactive oxygen species. |
| url | http://journal.bums.ac.ir/article-1-2997-en.html |
| work_keys_str_mv | AT vahidbagheri effectofhesperetinonthelevelofreactiveoxygenspeciesrosingastriccancerstemcellsshortcommunication AT mohammadzangooei effectofhesperetinonthelevelofreactiveoxygenspeciesrosingastriccancerstemcellsshortcommunication |