Sex-specific differences in the salivary microbiome of caries-active children
Background and Objectives: Dental caries is a chronic disease affecting young children and has multi-factorial risk factors. The purpose of this work was to identify sex-specific differences in the salivary microbiota within caries-active children. Design: Saliva specimens were collected from 85 chi...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Journal of Oral Microbiology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/20002297.2019.1653124 |
| _version_ | 1828860923255717888 |
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| author | Stephanie Ortiz Elisa Herrman Claudia Lyashenko Anne Purcell Kareem Raslan Brandon Khor Michael Snow Anna Forsyth Dongseok Choi Tom Maier Curtis A. Machida |
| author_facet | Stephanie Ortiz Elisa Herrman Claudia Lyashenko Anne Purcell Kareem Raslan Brandon Khor Michael Snow Anna Forsyth Dongseok Choi Tom Maier Curtis A. Machida |
| author_sort | Stephanie Ortiz |
| collection | DOAJ |
| description | Background and Objectives: Dental caries is a chronic disease affecting young children and has multi-factorial risk factors. The purpose of this work was to identify sex-specific differences in the salivary microbiota within caries-active children. Design: Saliva specimens were collected from 85 children (boys: 41; girls: 44) between the ages of 2-12 years. Salivary microbial DNA was subjected to PCR amplification using V3-V4 16S rDNA-specific primers and next-generation sequencing. Results: Significant sex differences in salivary microbiota were found between caries-active boys versus caries-active girls. Neisseria flavescens, Rothia aeria, and Haemophilus pittmaniae were found at significantly higher levels in caries-active boys. In contrast, Lactococcus lactis, Selenomonas species HOT 126, Actinobaculum species HOT 183, Veillonella parvula, and Alloprevotella species HOT 473 were found at significantly higher levels in caries-active girls. Conclusion: We have found the acid-generating, cariogenic Lactococcus lactis to be much more abundant in caries-active girls than caries-active boys, indicating that this microorganism may play a more significant role in shaping the cariogenic microbiome in girls. In addition, in caries-active girls, Alloprevotella species HOT 473 was the only species that exhibited both significant sex differences (4.4-fold difference; p=0.0003) as well as high abundance in numbers (1.85% of the total microbial population). |
| first_indexed | 2024-12-13T02:49:54Z |
| format | Article |
| id | doaj.art-4c431117982144f69448ef2056e71f56 |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-12-13T02:49:54Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-4c431117982144f69448ef2056e71f562022-12-22T00:02:05ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972019-01-0111110.1080/20002297.2019.16531241653124Sex-specific differences in the salivary microbiome of caries-active childrenStephanie Ortiz0Elisa Herrman1Claudia Lyashenko2Anne Purcell3Kareem Raslan4Brandon Khor5Michael Snow6Anna Forsyth7Dongseok Choi8Tom Maier9Curtis A. Machida10Oregon Health & Science University School of DentistryOregon Health & Science University School of DentistryOregon Health & Science University School of DentistryOregon Health & Science University School of DentistryOregon Health & Science University School of DentistryOregon Health & Science University School of DentistryOregon Health & Science University School of DentistryOregon Health & Science University School of DentistryKyunghee UniversityOregon Health & Science University School of DentistryOregon Health & Science University School of DentistryBackground and Objectives: Dental caries is a chronic disease affecting young children and has multi-factorial risk factors. The purpose of this work was to identify sex-specific differences in the salivary microbiota within caries-active children. Design: Saliva specimens were collected from 85 children (boys: 41; girls: 44) between the ages of 2-12 years. Salivary microbial DNA was subjected to PCR amplification using V3-V4 16S rDNA-specific primers and next-generation sequencing. Results: Significant sex differences in salivary microbiota were found between caries-active boys versus caries-active girls. Neisseria flavescens, Rothia aeria, and Haemophilus pittmaniae were found at significantly higher levels in caries-active boys. In contrast, Lactococcus lactis, Selenomonas species HOT 126, Actinobaculum species HOT 183, Veillonella parvula, and Alloprevotella species HOT 473 were found at significantly higher levels in caries-active girls. Conclusion: We have found the acid-generating, cariogenic Lactococcus lactis to be much more abundant in caries-active girls than caries-active boys, indicating that this microorganism may play a more significant role in shaping the cariogenic microbiome in girls. In addition, in caries-active girls, Alloprevotella species HOT 473 was the only species that exhibited both significant sex differences (4.4-fold difference; p=0.0003) as well as high abundance in numbers (1.85% of the total microbial population).http://dx.doi.org/10.1080/20002297.2019.1653124salivary microbiomesex differencesdental carieschildrenoral microbiome |
| spellingShingle | Stephanie Ortiz Elisa Herrman Claudia Lyashenko Anne Purcell Kareem Raslan Brandon Khor Michael Snow Anna Forsyth Dongseok Choi Tom Maier Curtis A. Machida Sex-specific differences in the salivary microbiome of caries-active children Journal of Oral Microbiology salivary microbiome sex differences dental caries children oral microbiome |
| title | Sex-specific differences in the salivary microbiome of caries-active children |
| title_full | Sex-specific differences in the salivary microbiome of caries-active children |
| title_fullStr | Sex-specific differences in the salivary microbiome of caries-active children |
| title_full_unstemmed | Sex-specific differences in the salivary microbiome of caries-active children |
| title_short | Sex-specific differences in the salivary microbiome of caries-active children |
| title_sort | sex specific differences in the salivary microbiome of caries active children |
| topic | salivary microbiome sex differences dental caries children oral microbiome |
| url | http://dx.doi.org/10.1080/20002297.2019.1653124 |
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