Taxonomic Identification of the Arctic Strain <i>Nocardioides Arcticus</i> Sp. Nov. and Global Transcriptomic Analysis in Response to Hydrogen Peroxide Stress
Microorganisms living in polar regions rely on specialized mechanisms to adapt to extreme environments. The study of their stress adaptation mechanisms is a hot topic in international microbiology research. In this study, a bacterial strain (Arc9.136) isolated from Arctic marine sediments was select...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-09-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/18/13943 |
| _version_ | 1797579721287925760 |
|---|---|
| author | Bailin Cong Hui Zhang Shuang Li Shenghao Liu Jing Lin Aifang Deng Wenqi Liu Yan Yang |
| author_facet | Bailin Cong Hui Zhang Shuang Li Shenghao Liu Jing Lin Aifang Deng Wenqi Liu Yan Yang |
| author_sort | Bailin Cong |
| collection | DOAJ |
| description | Microorganisms living in polar regions rely on specialized mechanisms to adapt to extreme environments. The study of their stress adaptation mechanisms is a hot topic in international microbiology research. In this study, a bacterial strain (Arc9.136) isolated from Arctic marine sediments was selected to implement polyphasic taxonomic identification based on factors such as genetic characteristics, physiological and biochemical properties, and chemical composition. The results showed that strain Arc9.136 is classified to the genus <i>Nocardioides</i>, for which the name <i>Nocardioides arcticus</i> sp. nov. is proposed. The ozone hole over the Arctic leads to increased ultraviolet (UV-B) radiation, and low temperatures lead to increased dissolved content in seawater. These extreme environmental conditions result in oxidative stress, inducing a strong response in microorganisms. Based on the functional classification of significantly differentially expressed genes under 1 mM H<sub>2</sub>O<sub>2</sub> stress, we suspect that Arc9.136 may respond to oxidative stress through the following strategies: (1) efficient utilization of various carbon sources to improve carbohydrate transport and metabolism; (2) altering ion transport and metabolism by decreasing the uptake of divalent iron (to avoid the Fenton reaction) and increasing the utilization of trivalent iron (to maintain intracellular iron homeostasis); (3) increasing the level of cell replication, DNA repair, and defense functions, repairing DNA damage caused by H<sub>2</sub>O<sub>2</sub>; (4) and changing the composition of lipids in the cell membrane and reducing the sensitivity of lipid peroxidation. This study provides insights into the stress resistance mechanisms of microorganisms in extreme environments and highlights the potential for developing low-temperature active microbial resources. |
| first_indexed | 2024-03-10T22:40:11Z |
| format | Article |
| id | doaj.art-4c49327265b647c1933a14ff61d0390e |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T22:40:11Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4c49327265b647c1933a14ff61d0390e2023-11-19T11:05:31ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181394310.3390/ijms241813943Taxonomic Identification of the Arctic Strain <i>Nocardioides Arcticus</i> Sp. Nov. and Global Transcriptomic Analysis in Response to Hydrogen Peroxide StressBailin Cong0Hui Zhang1Shuang Li2Shenghao Liu3Jing Lin4Aifang Deng5Wenqi Liu6Yan Yang7First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao 266100, ChinaCollege of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, ChinaFirst Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, ChinaFirst Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, ChinaFirst Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, ChinaFirst Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao 266100, ChinaMicroorganisms living in polar regions rely on specialized mechanisms to adapt to extreme environments. The study of their stress adaptation mechanisms is a hot topic in international microbiology research. In this study, a bacterial strain (Arc9.136) isolated from Arctic marine sediments was selected to implement polyphasic taxonomic identification based on factors such as genetic characteristics, physiological and biochemical properties, and chemical composition. The results showed that strain Arc9.136 is classified to the genus <i>Nocardioides</i>, for which the name <i>Nocardioides arcticus</i> sp. nov. is proposed. The ozone hole over the Arctic leads to increased ultraviolet (UV-B) radiation, and low temperatures lead to increased dissolved content in seawater. These extreme environmental conditions result in oxidative stress, inducing a strong response in microorganisms. Based on the functional classification of significantly differentially expressed genes under 1 mM H<sub>2</sub>O<sub>2</sub> stress, we suspect that Arc9.136 may respond to oxidative stress through the following strategies: (1) efficient utilization of various carbon sources to improve carbohydrate transport and metabolism; (2) altering ion transport and metabolism by decreasing the uptake of divalent iron (to avoid the Fenton reaction) and increasing the utilization of trivalent iron (to maintain intracellular iron homeostasis); (3) increasing the level of cell replication, DNA repair, and defense functions, repairing DNA damage caused by H<sub>2</sub>O<sub>2</sub>; (4) and changing the composition of lipids in the cell membrane and reducing the sensitivity of lipid peroxidation. This study provides insights into the stress resistance mechanisms of microorganisms in extreme environments and highlights the potential for developing low-temperature active microbial resources.https://www.mdpi.com/1422-0067/24/18/13943Arctic<i>Nocardioides</i> sp.antioxidationtrancriptome |
| spellingShingle | Bailin Cong Hui Zhang Shuang Li Shenghao Liu Jing Lin Aifang Deng Wenqi Liu Yan Yang Taxonomic Identification of the Arctic Strain <i>Nocardioides Arcticus</i> Sp. Nov. and Global Transcriptomic Analysis in Response to Hydrogen Peroxide Stress International Journal of Molecular Sciences Arctic <i>Nocardioides</i> sp. antioxidation trancriptome |
| title | Taxonomic Identification of the Arctic Strain <i>Nocardioides Arcticus</i> Sp. Nov. and Global Transcriptomic Analysis in Response to Hydrogen Peroxide Stress |
| title_full | Taxonomic Identification of the Arctic Strain <i>Nocardioides Arcticus</i> Sp. Nov. and Global Transcriptomic Analysis in Response to Hydrogen Peroxide Stress |
| title_fullStr | Taxonomic Identification of the Arctic Strain <i>Nocardioides Arcticus</i> Sp. Nov. and Global Transcriptomic Analysis in Response to Hydrogen Peroxide Stress |
| title_full_unstemmed | Taxonomic Identification of the Arctic Strain <i>Nocardioides Arcticus</i> Sp. Nov. and Global Transcriptomic Analysis in Response to Hydrogen Peroxide Stress |
| title_short | Taxonomic Identification of the Arctic Strain <i>Nocardioides Arcticus</i> Sp. Nov. and Global Transcriptomic Analysis in Response to Hydrogen Peroxide Stress |
| title_sort | taxonomic identification of the arctic strain i nocardioides arcticus i sp nov and global transcriptomic analysis in response to hydrogen peroxide stress |
| topic | Arctic <i>Nocardioides</i> sp. antioxidation trancriptome |
| url | https://www.mdpi.com/1422-0067/24/18/13943 |
| work_keys_str_mv | AT bailincong taxonomicidentificationofthearcticstraininocardioidesarcticusispnovandglobaltranscriptomicanalysisinresponsetohydrogenperoxidestress AT huizhang taxonomicidentificationofthearcticstraininocardioidesarcticusispnovandglobaltranscriptomicanalysisinresponsetohydrogenperoxidestress AT shuangli taxonomicidentificationofthearcticstraininocardioidesarcticusispnovandglobaltranscriptomicanalysisinresponsetohydrogenperoxidestress AT shenghaoliu taxonomicidentificationofthearcticstraininocardioidesarcticusispnovandglobaltranscriptomicanalysisinresponsetohydrogenperoxidestress AT jinglin taxonomicidentificationofthearcticstraininocardioidesarcticusispnovandglobaltranscriptomicanalysisinresponsetohydrogenperoxidestress AT aifangdeng taxonomicidentificationofthearcticstraininocardioidesarcticusispnovandglobaltranscriptomicanalysisinresponsetohydrogenperoxidestress AT wenqiliu taxonomicidentificationofthearcticstraininocardioidesarcticusispnovandglobaltranscriptomicanalysisinresponsetohydrogenperoxidestress AT yanyang taxonomicidentificationofthearcticstraininocardioidesarcticusispnovandglobaltranscriptomicanalysisinresponsetohydrogenperoxidestress |