Effect of indirubin on the expression of proinflammatory cytokines in keratinocytes
Objective: To investigate the effect of indirubin on the expression of proinflammatory cytokines in HaCaT cells, and to elucidate the mechanisms of Diqing ointment in the treatment of psoriasis. Methods: The human immortalized keratinocyte cell line, HaCaT cells, were used to observe the changes in...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
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editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology
2021-06-01
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| Series: | Pifu-xingbing zhenliaoxue zazhi |
| Subjects: | |
| Online Access: | http://pfxbzlx.gdvdc.com/CN/abstract/abstract1943.shtml |
| _version_ | 1811241943547510784 |
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| author | Qingwen LI Shougang LIU Jingxia LIN Yongfeng CHEN |
| author_facet | Qingwen LI Shougang LIU Jingxia LIN Yongfeng CHEN |
| author_sort | Qingwen LI |
| collection | DOAJ |
| description | Objective: To investigate the effect of indirubin on the expression of proinflammatory cytokines in HaCaT cells, and to elucidate the mechanisms of Diqing ointment in the treatment of psoriasis. Methods: The human immortalized keratinocyte cell line, HaCaT cells, were used to observe the changes in the expression levels of IL-1β, IL-6, and TNF-α in HaCaT cells after treatment with different concentrations of indirubin. HaCaT cells were divided into 4 groups. The secretion levels of IL-1β, IL-6, and TNF-α in the culture supernatants were detected by enzyme-linked immunosorbent assay ELISA, and the expression levels of IL-1β, IL-6, and TNF-α mRNA were detected by real-time PCR (RT-qPCR). Results: Expression levels of both mRNA and protein of IL-1β, IL-6, and TNF-α were significantly and dose-dependently decreased after treatment with indirubin. Conclusion: Indirubin, the main component of Qingdai ointment, can lower expression levels of pro-inflammatory cytokines such as IL-1β, IL-6, and TNF-α in keratinocytes, leading to the improvements of psoriasis. |
| first_indexed | 2024-04-12T13:44:42Z |
| format | Article |
| id | doaj.art-4c4a0c608bf04716ae7f19eb5716fad2 |
| institution | Directory Open Access Journal |
| issn | 1674-8468 |
| language | zho |
| last_indexed | 2024-04-12T13:44:42Z |
| publishDate | 2021-06-01 |
| publisher | editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology |
| record_format | Article |
| series | Pifu-xingbing zhenliaoxue zazhi |
| spelling | doaj.art-4c4a0c608bf04716ae7f19eb5716fad22022-12-22T03:30:44Zzhoeditoiral office of Journal of Diagnosis and Therapy on Dermato-venereologyPifu-xingbing zhenliaoxue zazhi1674-84682021-06-0128318218510.3969/j.issn.1674-8468.2021.03.005Effect of indirubin on the expression of proinflammatory cytokines in keratinocytesQingwen LI0Shougang LIU1Jingxia LIN2Yongfeng CHEN3Southern Medical University, Guangzhou 510515, ChinaDermatology Hospital, Southern Medical University, Guangzhou 510091, ChinaDermatology Hospital, Southern Medical University, Guangzhou 510091, ChinaDermatology Hospital, Southern Medical University, Guangzhou 510091, ChinaObjective: To investigate the effect of indirubin on the expression of proinflammatory cytokines in HaCaT cells, and to elucidate the mechanisms of Diqing ointment in the treatment of psoriasis. Methods: The human immortalized keratinocyte cell line, HaCaT cells, were used to observe the changes in the expression levels of IL-1β, IL-6, and TNF-α in HaCaT cells after treatment with different concentrations of indirubin. HaCaT cells were divided into 4 groups. The secretion levels of IL-1β, IL-6, and TNF-α in the culture supernatants were detected by enzyme-linked immunosorbent assay ELISA, and the expression levels of IL-1β, IL-6, and TNF-α mRNA were detected by real-time PCR (RT-qPCR). Results: Expression levels of both mRNA and protein of IL-1β, IL-6, and TNF-α were significantly and dose-dependently decreased after treatment with indirubin. Conclusion: Indirubin, the main component of Qingdai ointment, can lower expression levels of pro-inflammatory cytokines such as IL-1β, IL-6, and TNF-α in keratinocytes, leading to the improvements of psoriasis.http://pfxbzlx.gdvdc.com/CN/abstract/abstract1943.shtmlindirubinpsoriasishacat cellil-1βil-6tnf-α |
| spellingShingle | Qingwen LI Shougang LIU Jingxia LIN Yongfeng CHEN Effect of indirubin on the expression of proinflammatory cytokines in keratinocytes Pifu-xingbing zhenliaoxue zazhi indirubin psoriasis hacat cell il-1β il-6 tnf-α |
| title | Effect of indirubin on the expression of proinflammatory cytokines in keratinocytes |
| title_full | Effect of indirubin on the expression of proinflammatory cytokines in keratinocytes |
| title_fullStr | Effect of indirubin on the expression of proinflammatory cytokines in keratinocytes |
| title_full_unstemmed | Effect of indirubin on the expression of proinflammatory cytokines in keratinocytes |
| title_short | Effect of indirubin on the expression of proinflammatory cytokines in keratinocytes |
| title_sort | effect of indirubin on the expression of proinflammatory cytokines in keratinocytes |
| topic | indirubin psoriasis hacat cell il-1β il-6 tnf-α |
| url | http://pfxbzlx.gdvdc.com/CN/abstract/abstract1943.shtml |
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