Data for the optimization of conditions for meat species identification using ultra-fast multiplex direct-convection PCR
This article contains data related to the research article entitled “Ultra-fast DNA-based multiplex convection PCR method for meat species identification with possible on-site applications” (Song et al., 2017 [1]). Direct PCR that does not require prior DNA extraction is critical for ultra-fast mole...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-02-01
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| Series: | Data in Brief |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340917306005 |
| _version_ | 1828378046946607104 |
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| author | Kyung-Young Song Hyun Jin Hwang Jeong Hee Kim |
| author_facet | Kyung-Young Song Hyun Jin Hwang Jeong Hee Kim |
| author_sort | Kyung-Young Song |
| collection | DOAJ |
| description | This article contains data related to the research article entitled “Ultra-fast DNA-based multiplex convection PCR method for meat species identification with possible on-site applications” (Song et al., 2017 [1]). Direct PCR that does not require prior DNA extraction is critical for ultra-fast molecular detection of meat species. We successfully acquired DNA by swab sampling in Taq DNA polymerase buffer. To reduce DNA sample preparation time, proteinase K incubation (0.2 μg/mL) and heat inactivation times were decreased to 10 min and 1 min, respectively. The analysis of swabbed DNA samples from mixed meat could differentiate meat species within the mixed sample. The swabbed DNA samples could be diluted 100 times without losing detection sensitivity. Keywords: DNA-based, Multiplex, Meat species, Convection PCR |
| first_indexed | 2024-04-14T08:20:51Z |
| format | Article |
| id | doaj.art-4c4afb961a13485fbeaf789f62c767a4 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-04-14T08:20:51Z |
| publishDate | 2018-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-4c4afb961a13485fbeaf789f62c767a42022-12-22T02:04:12ZengElsevierData in Brief2352-34092018-02-01161518Data for the optimization of conditions for meat species identification using ultra-fast multiplex direct-convection PCRKyung-Young Song0Hyun Jin Hwang1Jeong Hee Kim2R&D center, Ahram Biosystems Inc., Seoul 133-120, Republic of Korea; Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul 130-701, Republic of KoreaR&D center, Ahram Biosystems Inc., Seoul 133-120, Republic of KoreaDepartment of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul 130-701, Republic of Korea; Department of Oral Biochemistry and Molecular Biology, Kyung Hee University, Seoul 130-701, Republic of Korea; Corresponding author at: Department of Life and Nanopharmaceutical Sciences, Graduate School, and Department of Oral Biochemistry and Molecular Biology, Kyung Hee University, Seoul 130-701, KoreaThis article contains data related to the research article entitled “Ultra-fast DNA-based multiplex convection PCR method for meat species identification with possible on-site applications” (Song et al., 2017 [1]). Direct PCR that does not require prior DNA extraction is critical for ultra-fast molecular detection of meat species. We successfully acquired DNA by swab sampling in Taq DNA polymerase buffer. To reduce DNA sample preparation time, proteinase K incubation (0.2 μg/mL) and heat inactivation times were decreased to 10 min and 1 min, respectively. The analysis of swabbed DNA samples from mixed meat could differentiate meat species within the mixed sample. The swabbed DNA samples could be diluted 100 times without losing detection sensitivity. Keywords: DNA-based, Multiplex, Meat species, Convection PCRhttp://www.sciencedirect.com/science/article/pii/S2352340917306005 |
| spellingShingle | Kyung-Young Song Hyun Jin Hwang Jeong Hee Kim Data for the optimization of conditions for meat species identification using ultra-fast multiplex direct-convection PCR Data in Brief |
| title | Data for the optimization of conditions for meat species identification using ultra-fast multiplex direct-convection PCR |
| title_full | Data for the optimization of conditions for meat species identification using ultra-fast multiplex direct-convection PCR |
| title_fullStr | Data for the optimization of conditions for meat species identification using ultra-fast multiplex direct-convection PCR |
| title_full_unstemmed | Data for the optimization of conditions for meat species identification using ultra-fast multiplex direct-convection PCR |
| title_short | Data for the optimization of conditions for meat species identification using ultra-fast multiplex direct-convection PCR |
| title_sort | data for the optimization of conditions for meat species identification using ultra fast multiplex direct convection pcr |
| url | http://www.sciencedirect.com/science/article/pii/S2352340917306005 |
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