Intranasal Administration of Undifferentiated Oligodendrocyte Lineage Cells as a Potential Approach to Deliver Oligodendrocyte Precursor Cells into Brain
Oligodendrocyte precursor cell (OPC) migration is a mechanism involved in remyelination; these cells migrate from niches in the adult CNS. However, age and disease reduce the pool of OPCs; as a result, the remyelination capacity of the CNS decreases over time. Several experimental studies have intro...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/19/10738 |
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| author | Ulises Gómez-Pinedo Jordi A. Matías-Guiu María Soledad Benito-Martín Lidia Moreno-Jiménez Inmaculada Sanclemente-Alamán Belen Selma-Calvo Sara Pérez-Suarez Francisco Sancho-Bielsa Alejandro Canales-Aguirre Juan Carlos Mateos-Díaz Mercedes A. Hernández-Sapiéns Edwin E. Reza-Zaldívar Doddy Denise Ojeda-Hernández Lucía Vidorreta-Ballesteros Paloma Montero-Escribano Jorge Matías-Guiu |
| author_facet | Ulises Gómez-Pinedo Jordi A. Matías-Guiu María Soledad Benito-Martín Lidia Moreno-Jiménez Inmaculada Sanclemente-Alamán Belen Selma-Calvo Sara Pérez-Suarez Francisco Sancho-Bielsa Alejandro Canales-Aguirre Juan Carlos Mateos-Díaz Mercedes A. Hernández-Sapiéns Edwin E. Reza-Zaldívar Doddy Denise Ojeda-Hernández Lucía Vidorreta-Ballesteros Paloma Montero-Escribano Jorge Matías-Guiu |
| author_sort | Ulises Gómez-Pinedo |
| collection | DOAJ |
| description | Oligodendrocyte precursor cell (OPC) migration is a mechanism involved in remyelination; these cells migrate from niches in the adult CNS. However, age and disease reduce the pool of OPCs; as a result, the remyelination capacity of the CNS decreases over time. Several experimental studies have introduced OPCs to the brain via direct injection or intrathecal administration. In this study, we used the nose-to brain pathway to deliver oligodendrocyte lineage cells (human oligodendroglioma (HOG) cells), which behave similarly to OPCs in vitro. To this end, we administered GFP-labelled HOG cells intranasally to experimental animals, which were subsequently euthanised at 30 or 60 days. Our results show that the intranasal route is a viable route to the CNS and that HOG cells administered intranasally migrate preferentially to niches of OPCs (clusters created during embryonic development and adult life). Our study provides evidence, albeit limited, that HOG cells either form clusters or adhere to clusters of OPCs in the brains of experimental animals. |
| first_indexed | 2024-03-10T06:58:45Z |
| format | Article |
| id | doaj.art-4c4e5050747b4a5086512dfb2d4f00f2 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T06:58:45Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4c4e5050747b4a5086512dfb2d4f00f22023-11-22T16:14:36ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-10-0122191073810.3390/ijms221910738Intranasal Administration of Undifferentiated Oligodendrocyte Lineage Cells as a Potential Approach to Deliver Oligodendrocyte Precursor Cells into BrainUlises Gómez-Pinedo0Jordi A. Matías-Guiu1María Soledad Benito-Martín2Lidia Moreno-Jiménez3Inmaculada Sanclemente-Alamán4Belen Selma-Calvo5Sara Pérez-Suarez6Francisco Sancho-Bielsa7Alejandro Canales-Aguirre8Juan Carlos Mateos-Díaz9Mercedes A. Hernández-Sapiéns10Edwin E. Reza-Zaldívar11Doddy Denise Ojeda-Hernández12Lucía Vidorreta-Ballesteros13Paloma Montero-Escribano14Jorge Matías-Guiu15Laboratory of Neurobiology, Institute of Neurosciences, Health Research Institute of the Hospital Clínico San Carlos, Universidad Complutense de Madrid, 28040 Madrid, SpainDepartment of Neurology, Institute of Neurosciences IdISSC, Hospital Clinico San Carlos, Universidad Complutense de Madrid, 28040 Madrid, SpainLaboratory of Neurobiology, Institute of Neurosciences, Health Research Institute of the Hospital Clínico San Carlos, Universidad Complutense de Madrid, 28040 Madrid, SpainLaboratory of Neurobiology, Institute of Neurosciences, Health Research Institute of the Hospital Clínico San Carlos, Universidad Complutense de Madrid, 28040 Madrid, SpainLaboratory of Neurobiology, Institute of Neurosciences, Health Research Institute of the Hospital Clínico San Carlos, Universidad Complutense de Madrid, 28040 Madrid, SpainLaboratory of Neurobiology, Institute of Neurosciences, Health Research Institute of the Hospital Clínico San Carlos, Universidad Complutense de Madrid, 28040 Madrid, SpainLaboratory of Neurobiology, Institute of Neurosciences, Health Research Institute of the Hospital Clínico San Carlos, Universidad Complutense de Madrid, 28040 Madrid, SpainDepartment of Physiology, Ciudad Real School of Medicine, Universidad de Castilla-La Mancha, 13005 Ciudad Real, SpainPreclinical Evaluation Unit, Medical and Pharmaceutical Biotechnology Unit, CIATEJ-CONACyT, Guadalajara 44270, MexicoDepartment of Industrial Biotechnology, CIATEJ-CONACyT, Zapopan 45019, MexicoPreclinical Evaluation Unit, Medical and Pharmaceutical Biotechnology Unit, CIATEJ-CONACyT, Guadalajara 44270, MexicoPreclinical Evaluation Unit, Medical and Pharmaceutical Biotechnology Unit, CIATEJ-CONACyT, Guadalajara 44270, MexicoPreclinical Evaluation Unit, Medical and Pharmaceutical Biotechnology Unit, CIATEJ-CONACyT, Guadalajara 44270, MexicoDepartment of Neurology, Institute of Neurosciences IdISSC, Hospital Clinico San Carlos, Universidad Complutense de Madrid, 28040 Madrid, SpainDepartment of Neurology, Institute of Neurosciences IdISSC, Hospital Clinico San Carlos, Universidad Complutense de Madrid, 28040 Madrid, SpainLaboratory of Neurobiology, Institute of Neurosciences, Health Research Institute of the Hospital Clínico San Carlos, Universidad Complutense de Madrid, 28040 Madrid, SpainOligodendrocyte precursor cell (OPC) migration is a mechanism involved in remyelination; these cells migrate from niches in the adult CNS. However, age and disease reduce the pool of OPCs; as a result, the remyelination capacity of the CNS decreases over time. Several experimental studies have introduced OPCs to the brain via direct injection or intrathecal administration. In this study, we used the nose-to brain pathway to deliver oligodendrocyte lineage cells (human oligodendroglioma (HOG) cells), which behave similarly to OPCs in vitro. To this end, we administered GFP-labelled HOG cells intranasally to experimental animals, which were subsequently euthanised at 30 or 60 days. Our results show that the intranasal route is a viable route to the CNS and that HOG cells administered intranasally migrate preferentially to niches of OPCs (clusters created during embryonic development and adult life). Our study provides evidence, albeit limited, that HOG cells either form clusters or adhere to clusters of OPCs in the brains of experimental animals.https://www.mdpi.com/1422-0067/22/19/10738multiple sclerosisHOG cellsoligodendrocyte precursor cellsoligodendrocytesintranasal administrationdemyelination |
| spellingShingle | Ulises Gómez-Pinedo Jordi A. Matías-Guiu María Soledad Benito-Martín Lidia Moreno-Jiménez Inmaculada Sanclemente-Alamán Belen Selma-Calvo Sara Pérez-Suarez Francisco Sancho-Bielsa Alejandro Canales-Aguirre Juan Carlos Mateos-Díaz Mercedes A. Hernández-Sapiéns Edwin E. Reza-Zaldívar Doddy Denise Ojeda-Hernández Lucía Vidorreta-Ballesteros Paloma Montero-Escribano Jorge Matías-Guiu Intranasal Administration of Undifferentiated Oligodendrocyte Lineage Cells as a Potential Approach to Deliver Oligodendrocyte Precursor Cells into Brain International Journal of Molecular Sciences multiple sclerosis HOG cells oligodendrocyte precursor cells oligodendrocytes intranasal administration demyelination |
| title | Intranasal Administration of Undifferentiated Oligodendrocyte Lineage Cells as a Potential Approach to Deliver Oligodendrocyte Precursor Cells into Brain |
| title_full | Intranasal Administration of Undifferentiated Oligodendrocyte Lineage Cells as a Potential Approach to Deliver Oligodendrocyte Precursor Cells into Brain |
| title_fullStr | Intranasal Administration of Undifferentiated Oligodendrocyte Lineage Cells as a Potential Approach to Deliver Oligodendrocyte Precursor Cells into Brain |
| title_full_unstemmed | Intranasal Administration of Undifferentiated Oligodendrocyte Lineage Cells as a Potential Approach to Deliver Oligodendrocyte Precursor Cells into Brain |
| title_short | Intranasal Administration of Undifferentiated Oligodendrocyte Lineage Cells as a Potential Approach to Deliver Oligodendrocyte Precursor Cells into Brain |
| title_sort | intranasal administration of undifferentiated oligodendrocyte lineage cells as a potential approach to deliver oligodendrocyte precursor cells into brain |
| topic | multiple sclerosis HOG cells oligodendrocyte precursor cells oligodendrocytes intranasal administration demyelination |
| url | https://www.mdpi.com/1422-0067/22/19/10738 |
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