Effects of environmental variables on abundance of ammonia-oxidizing communities in sediments of Luotian River, China
Ammonia-oxidizing communities play important functional roles in the nitrification. However, environmental stresses can significantly affect this process by controlling the abundant communities of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) communities. In this study, we exa...
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PeerJ Inc.
2020-01-01
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| author | Amjed Ginawi Lixiao Wang Huading Wang Bingbing Yu Yan Yunjun |
| author_facet | Amjed Ginawi Lixiao Wang Huading Wang Bingbing Yu Yan Yunjun |
| author_sort | Amjed Ginawi |
| collection | DOAJ |
| description | Ammonia-oxidizing communities play important functional roles in the nitrification. However, environmental stresses can significantly affect this process by controlling the abundant communities of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) communities. In this study, we examined the abundance variations of ammonia-oxidizing communities using quantitative polymerase chain reaction (qPCR) and terminal-restriction fragment length polymorphism (T-RFLP) in a typical subtropical river, Luotian County, South Dabie Mountains, China. Clone libraries were conducted to evaluate the community structure and abundance of AOA and AOB in sediments. Results showed that Nitrososphaera sp and Nitrosopumilus sp were the most dominant AOA. The abundance of the AOA and AOB amoA gene ranged from 5.28 × 108 gene copies (g-soil−1) to 2.23 × 108 gene copies (g-soil−1) and 5.45 × 108 gene copies (g-soil−1) to 3.30 × 107 gene copies (g-soil−1), respectively. Five environmental variables, namely, ORP, DO, NO ${}_{3}^{-}$ 3 − , Temp, and NH ${}_{4}^{+}$ 4 + were played a major function in microbial communities of AOA and AOB in sediments. The T-RFLP profiles of AOA showed that 488 and 116 bp T-RFs were dominated. Overall, the results of this study showed that anthropogenic activities andenvironmental stress in rivers can alter the structure and function of microbes in their variable environment. |
| first_indexed | 2024-03-09T08:20:35Z |
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| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T08:20:35Z |
| publishDate | 2020-01-01 |
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| spelling | doaj.art-4c501d7c8ade434cb20a4619bdc1f8f52023-12-02T21:50:03ZengPeerJ Inc.PeerJ2167-83592020-01-018e825610.7717/peerj.8256Effects of environmental variables on abundance of ammonia-oxidizing communities in sediments of Luotian River, ChinaAmjed Ginawi0Lixiao Wang1Huading Wang2Bingbing Yu3Yan Yunjun4Key Lab of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaKey Lab of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaKey Lab of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaKey Lab of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaKey Lab of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, ChinaAmmonia-oxidizing communities play important functional roles in the nitrification. However, environmental stresses can significantly affect this process by controlling the abundant communities of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) communities. In this study, we examined the abundance variations of ammonia-oxidizing communities using quantitative polymerase chain reaction (qPCR) and terminal-restriction fragment length polymorphism (T-RFLP) in a typical subtropical river, Luotian County, South Dabie Mountains, China. Clone libraries were conducted to evaluate the community structure and abundance of AOA and AOB in sediments. Results showed that Nitrososphaera sp and Nitrosopumilus sp were the most dominant AOA. The abundance of the AOA and AOB amoA gene ranged from 5.28 × 108 gene copies (g-soil−1) to 2.23 × 108 gene copies (g-soil−1) and 5.45 × 108 gene copies (g-soil−1) to 3.30 × 107 gene copies (g-soil−1), respectively. Five environmental variables, namely, ORP, DO, NO ${}_{3}^{-}$ 3 − , Temp, and NH ${}_{4}^{+}$ 4 + were played a major function in microbial communities of AOA and AOB in sediments. The T-RFLP profiles of AOA showed that 488 and 116 bp T-RFs were dominated. Overall, the results of this study showed that anthropogenic activities andenvironmental stress in rivers can alter the structure and function of microbes in their variable environment.https://peerj.com/articles/8256.pdfLuotian riverAmmonia-oxidizing archaeaFunctional marker genesAmmonia-oxidizing bacteria |
| spellingShingle | Amjed Ginawi Lixiao Wang Huading Wang Bingbing Yu Yan Yunjun Effects of environmental variables on abundance of ammonia-oxidizing communities in sediments of Luotian River, China PeerJ Luotian river Ammonia-oxidizing archaea Functional marker genes Ammonia-oxidizing bacteria |
| title | Effects of environmental variables on abundance of ammonia-oxidizing communities in sediments of Luotian River, China |
| title_full | Effects of environmental variables on abundance of ammonia-oxidizing communities in sediments of Luotian River, China |
| title_fullStr | Effects of environmental variables on abundance of ammonia-oxidizing communities in sediments of Luotian River, China |
| title_full_unstemmed | Effects of environmental variables on abundance of ammonia-oxidizing communities in sediments of Luotian River, China |
| title_short | Effects of environmental variables on abundance of ammonia-oxidizing communities in sediments of Luotian River, China |
| title_sort | effects of environmental variables on abundance of ammonia oxidizing communities in sediments of luotian river china |
| topic | Luotian river Ammonia-oxidizing archaea Functional marker genes Ammonia-oxidizing bacteria |
| url | https://peerj.com/articles/8256.pdf |
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