Differential role of GPR142 in tryptophan-mediated enhancement of insulin secretion in obese and lean mice.
Tryptophan is reportedly the most potent agonist for GPR142. Glucose-stimulated insulin secretion (GSIS) from pancreatic beta cells are enhanced by GPR142-mediated signal. It is not clear, however, if GPR142-mediated signals is solely attributable to GSIS enhancement after tryptophan load in various...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5995358?pdf=render |
| _version_ | 1818172054779723776 |
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| author | Yoko Ueda Hiroshi Iwakura Mika Bando Asako Doi Hiroyuki Ariyasu Hidefumi Inaba Shuhei Morita Takashi Akamizu |
| author_facet | Yoko Ueda Hiroshi Iwakura Mika Bando Asako Doi Hiroyuki Ariyasu Hidefumi Inaba Shuhei Morita Takashi Akamizu |
| author_sort | Yoko Ueda |
| collection | DOAJ |
| description | Tryptophan is reportedly the most potent agonist for GPR142. Glucose-stimulated insulin secretion (GSIS) from pancreatic beta cells are enhanced by GPR142-mediated signal. It is not clear, however, if GPR142-mediated signals is solely attributable to GSIS enhancement after tryptophan load in various pathophysiological settings. This study aims to reveal the significance of GPR142 signaling in tryptophan-mediated GSIS enhancement in normal and obese mice. Tryptophan significantly improved glucose tolerance in both lean and DIO mice, but the extent of improvement was bigger in DIO mice with augmented glucose-stimulated insulin secretion (GSIS) enhancement. The same results were obtained in ob/ob mice. GPR142 deletion almost completely blocked tryptophan actions in lean mice, suggesting that GPR142 signaling was solely responsible for the GSIS enhancement. In obese GPR142KO mice, however, a significant amount of tryptophan effects were still observed. Calcium-sensing receptors (CaSR) are also known to recognize tryptophan as ligand. Expression levels of CaSR were significantly elevated in the pancreas of DIO mice, and CaSR antagonist further blocked tryptophan's actions in DIO mice with GPR142 deletion. Although GPR142 signaling had a major role in tryptophan recognition for the enhancement of GSIS in lean mice, other pathways including CaSR signaling also had a significant role in obese mice, which seemed to contribute to the augmented enhancement of GSIS by tryptophan in these animals. |
| first_indexed | 2024-12-11T19:06:31Z |
| format | Article |
| id | doaj.art-4c50f93646854e6299ff181896628e45 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T19:06:31Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4c50f93646854e6299ff181896628e452022-12-22T00:53:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01136e019876210.1371/journal.pone.0198762Differential role of GPR142 in tryptophan-mediated enhancement of insulin secretion in obese and lean mice.Yoko UedaHiroshi IwakuraMika BandoAsako DoiHiroyuki AriyasuHidefumi InabaShuhei MoritaTakashi AkamizuTryptophan is reportedly the most potent agonist for GPR142. Glucose-stimulated insulin secretion (GSIS) from pancreatic beta cells are enhanced by GPR142-mediated signal. It is not clear, however, if GPR142-mediated signals is solely attributable to GSIS enhancement after tryptophan load in various pathophysiological settings. This study aims to reveal the significance of GPR142 signaling in tryptophan-mediated GSIS enhancement in normal and obese mice. Tryptophan significantly improved glucose tolerance in both lean and DIO mice, but the extent of improvement was bigger in DIO mice with augmented glucose-stimulated insulin secretion (GSIS) enhancement. The same results were obtained in ob/ob mice. GPR142 deletion almost completely blocked tryptophan actions in lean mice, suggesting that GPR142 signaling was solely responsible for the GSIS enhancement. In obese GPR142KO mice, however, a significant amount of tryptophan effects were still observed. Calcium-sensing receptors (CaSR) are also known to recognize tryptophan as ligand. Expression levels of CaSR were significantly elevated in the pancreas of DIO mice, and CaSR antagonist further blocked tryptophan's actions in DIO mice with GPR142 deletion. Although GPR142 signaling had a major role in tryptophan recognition for the enhancement of GSIS in lean mice, other pathways including CaSR signaling also had a significant role in obese mice, which seemed to contribute to the augmented enhancement of GSIS by tryptophan in these animals.http://europepmc.org/articles/PMC5995358?pdf=render |
| spellingShingle | Yoko Ueda Hiroshi Iwakura Mika Bando Asako Doi Hiroyuki Ariyasu Hidefumi Inaba Shuhei Morita Takashi Akamizu Differential role of GPR142 in tryptophan-mediated enhancement of insulin secretion in obese and lean mice. PLoS ONE |
| title | Differential role of GPR142 in tryptophan-mediated enhancement of insulin secretion in obese and lean mice. |
| title_full | Differential role of GPR142 in tryptophan-mediated enhancement of insulin secretion in obese and lean mice. |
| title_fullStr | Differential role of GPR142 in tryptophan-mediated enhancement of insulin secretion in obese and lean mice. |
| title_full_unstemmed | Differential role of GPR142 in tryptophan-mediated enhancement of insulin secretion in obese and lean mice. |
| title_short | Differential role of GPR142 in tryptophan-mediated enhancement of insulin secretion in obese and lean mice. |
| title_sort | differential role of gpr142 in tryptophan mediated enhancement of insulin secretion in obese and lean mice |
| url | http://europepmc.org/articles/PMC5995358?pdf=render |
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