Downregulation Of Extracellular Matrix And Cell Adhesion Molecules In Cumulus Cells Of Infertile Polycystic Ovary Syndrome Women With And Without Insulin Resistance
Objective The extracellular matrix (ECM) of the cumulus oocyte complex (COC) is composed of several molecules that have different roles during follicle development. This study aims to explore gene expression profiles for ECM and cell adhesion molecules in the cumulus cells of polycystic ovary syndr...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Royan Institute (ACECR), Tehran
2018-12-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | https://celljournal.org/journal/article/abstract/5576 |
| _version_ | 1818273544202616832 |
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| author | Fatemeh Hassani Shahrbanoo Oryan Poopak Eftekhari-Yazdi Masood Bazrgar Ashraf Moini Nahid Nasiri Ali Sharifi-Zarchi |
| author_facet | Fatemeh Hassani Shahrbanoo Oryan Poopak Eftekhari-Yazdi Masood Bazrgar Ashraf Moini Nahid Nasiri Ali Sharifi-Zarchi |
| author_sort | Fatemeh Hassani |
| collection | DOAJ |
| description | Objective
The extracellular matrix (ECM) of the cumulus oocyte complex (COC) is composed of several molecules that have different roles during follicle development. This study aims to explore gene expression profiles for ECM and cell adhesion molecules in the cumulus cells of polycystic ovary syndrome (PCOS) patients based on their insulin sensitivity following controlled ovarian stimulation (COS).
Materials And Methods
In this prospective case-control study enrolled 23 women less than 36 years of age who participated in an intracytoplasmic sperm injection (ICSI) program. Patients were subdivided into 3 groups: control (n=8, fertile women with male infertility history), insulin resistant (IR) PCOS (n=7), and insulin sensitive (IS) PCOS (n=8). We compared 84 ECM component and adhesion molecule gene expressions by quantitative real-time polymerase chain reaction array (qPCR-array) among the groups.
Results
We noted that 21 of the 84 studied genes differentially expressed among the groups, from which 18 of these genes downregulated. Overall, comparison of PCOS cases with controls showed downregulation of extracellular matrix protein 1 (ECM1); catenin (cadherin-associated protein), alpha 1 (CTNNA1); integrin, alpha 5 (ITGA5); laminin, alpha 3 (LAMA3); laminin, beta 1 (LAMB1); fibronectin 1 (FN1); and integrin, alpha 7 (ITGA7). In the IS group, there was upregulation of ADAM metallopeptidase with thrombospondin type 1 motif, 8 (ADAMTS8) and neural cell adhesion molecule 1 (NCAM1) compared with the controls (P<0.05).
Conclusion
Downregulation of ECM and cell adhesion molecules seem to be related to PCOS. Gene expression profile alterations in cumulus cells from both the IS and IR groups of PCOS patients seems to be involved in the composition and regulation of ECM during the ovulation process. This study highlights the association of ECM gene alteration as a viewpoint for additional understanding of the etiology of PCOS. |
| first_indexed | 2024-12-12T21:59:39Z |
| format | Article |
| id | doaj.art-4c54936c7ba942eb8d8f589285b18a27 |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-12-12T21:59:39Z |
| publishDate | 2018-12-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-4c54936c7ba942eb8d8f589285b18a272022-12-22T00:10:33ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142018-12-01211354210.22074/cellj.2019.5576Downregulation Of Extracellular Matrix And Cell Adhesion Molecules In Cumulus Cells Of Infertile Polycystic Ovary Syndrome Women With And Without Insulin Resistance Fatemeh Hassani0Shahrbanoo Oryan1Poopak Eftekhari-Yazdi2Masood Bazrgar3Ashraf Moini4Nahid Nasiri5Ali Sharifi-Zarchi6Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, IranDepartment of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, IranDepartment of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranDepartment of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranDepartment of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranDepartment of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranDepartment of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, IranObjective The extracellular matrix (ECM) of the cumulus oocyte complex (COC) is composed of several molecules that have different roles during follicle development. This study aims to explore gene expression profiles for ECM and cell adhesion molecules in the cumulus cells of polycystic ovary syndrome (PCOS) patients based on their insulin sensitivity following controlled ovarian stimulation (COS). Materials And Methods In this prospective case-control study enrolled 23 women less than 36 years of age who participated in an intracytoplasmic sperm injection (ICSI) program. Patients were subdivided into 3 groups: control (n=8, fertile women with male infertility history), insulin resistant (IR) PCOS (n=7), and insulin sensitive (IS) PCOS (n=8). We compared 84 ECM component and adhesion molecule gene expressions by quantitative real-time polymerase chain reaction array (qPCR-array) among the groups. Results We noted that 21 of the 84 studied genes differentially expressed among the groups, from which 18 of these genes downregulated. Overall, comparison of PCOS cases with controls showed downregulation of extracellular matrix protein 1 (ECM1); catenin (cadherin-associated protein), alpha 1 (CTNNA1); integrin, alpha 5 (ITGA5); laminin, alpha 3 (LAMA3); laminin, beta 1 (LAMB1); fibronectin 1 (FN1); and integrin, alpha 7 (ITGA7). In the IS group, there was upregulation of ADAM metallopeptidase with thrombospondin type 1 motif, 8 (ADAMTS8) and neural cell adhesion molecule 1 (NCAM1) compared with the controls (P<0.05). Conclusion Downregulation of ECM and cell adhesion molecules seem to be related to PCOS. Gene expression profile alterations in cumulus cells from both the IS and IR groups of PCOS patients seems to be involved in the composition and regulation of ECM during the ovulation process. This study highlights the association of ECM gene alteration as a viewpoint for additional understanding of the etiology of PCOS.https://celljournal.org/journal/article/abstract/5576Cumulus CellsExtracellular MatrixGene ExpressionInsulin ResistancePolycystic Ovary Syndrome |
| spellingShingle | Fatemeh Hassani Shahrbanoo Oryan Poopak Eftekhari-Yazdi Masood Bazrgar Ashraf Moini Nahid Nasiri Ali Sharifi-Zarchi Downregulation Of Extracellular Matrix And Cell Adhesion Molecules In Cumulus Cells Of Infertile Polycystic Ovary Syndrome Women With And Without Insulin Resistance Cell Journal Cumulus Cells Extracellular Matrix Gene Expression Insulin Resistance Polycystic Ovary Syndrome |
| title | Downregulation Of Extracellular Matrix And Cell Adhesion Molecules In Cumulus Cells Of Infertile Polycystic Ovary Syndrome Women With And Without Insulin Resistance |
| title_full | Downregulation Of Extracellular Matrix And Cell Adhesion Molecules In Cumulus Cells Of Infertile Polycystic Ovary Syndrome Women With And Without Insulin Resistance |
| title_fullStr | Downregulation Of Extracellular Matrix And Cell Adhesion Molecules In Cumulus Cells Of Infertile Polycystic Ovary Syndrome Women With And Without Insulin Resistance |
| title_full_unstemmed | Downregulation Of Extracellular Matrix And Cell Adhesion Molecules In Cumulus Cells Of Infertile Polycystic Ovary Syndrome Women With And Without Insulin Resistance |
| title_short | Downregulation Of Extracellular Matrix And Cell Adhesion Molecules In Cumulus Cells Of Infertile Polycystic Ovary Syndrome Women With And Without Insulin Resistance |
| title_sort | downregulation of extracellular matrix and cell adhesion molecules in cumulus cells of infertile polycystic ovary syndrome women with and without insulin resistance |
| topic | Cumulus Cells Extracellular Matrix Gene Expression Insulin Resistance Polycystic Ovary Syndrome |
| url | https://celljournal.org/journal/article/abstract/5576 |
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