Body adiposity but not insulin resistance is associated with -675 4G/5G polymorphism in the PAI-1 gene in a sample of Mexican children

Objective: To assess whether the -675 4G/5G polymorphism in the plasminogen activator inhibitor-1 gene is associated with obesity and insulin resistance in Mexican children. Methods: A cross-sectional study was performed in 174 children, 89 with normal-weight and 85 with obesity, aged from 6 to 13 y...

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Main Authors: Ulises de la Cruz-Mosso, José Francisco Muñoz-Valle, Aralia Berenice Salgado-Bernabé, Natividad Castro-Alarcón, Lorenzo Salgado-Goytia, José Sánchez-Corona, Silvia Esperanza Flores-Martínez, Isela Parra-Rojas
Format: Article
Language:Portuguese
Published: Brazilian Society of Pediatrics 2013-09-01
Series:Jornal de Pediatria (Versão em Português)
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2255553613000955
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author Ulises de la Cruz-Mosso
José Francisco Muñoz-Valle
Aralia Berenice Salgado-Bernabé
Natividad Castro-Alarcón
Lorenzo Salgado-Goytia
José Sánchez-Corona
Silvia Esperanza Flores-Martínez
Isela Parra-Rojas
author_facet Ulises de la Cruz-Mosso
José Francisco Muñoz-Valle
Aralia Berenice Salgado-Bernabé
Natividad Castro-Alarcón
Lorenzo Salgado-Goytia
José Sánchez-Corona
Silvia Esperanza Flores-Martínez
Isela Parra-Rojas
author_sort Ulises de la Cruz-Mosso
collection DOAJ
description Objective: To assess whether the -675 4G/5G polymorphism in the plasminogen activator inhibitor-1 gene is associated with obesity and insulin resistance in Mexican children. Methods: A cross-sectional study was performed in 174 children, 89 with normal-weight and 85 with obesity, aged from 6 to 13 years. All children were from state of Guerrero, and recruited from three primary schools in the city of Chilpancingo, Mexico. Insulin levels were determined by immunoenzymatic assay. The homeostasis model assessment was used to determine insulin resistance. The -675 4G/5G polymorphism in PAI-1 gene was analyzed by polymerase chain reaction-restriction fragment length polymorphism. Results: The prevalence of insulin resistance in the obese group was higher (49.41%) than in the normal-weight group (16.85%). The 4G/5G PAI-1 polymorphism was found in Hardy Weinberg equilibrium. The 4G/5G genotype contributed to a significant increase in waist-hip ratio (b = 0.02, p = 0.006), waist circumference (b = 4.42, p = 0.009), and subscapular skinfold thickness (b = 1.79, p = 0.04); however, it was not related with insulin resistance. Conclusion: The -675 4G/5G genotype of PAI-1 gene was associated with increase of body adiposity in Mexican children.
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spelling doaj.art-4c55b882df5749858a816c3f88890b882022-12-22T03:56:01ZporBrazilian Society of PediatricsJornal de Pediatria (Versão em Português)2255-55362013-09-0189549249810.1016/j.jpedp.2013.01.005Body adiposity but not insulin resistance is associated with -675 4G/5G polymorphism in the PAI-1 gene in a sample of Mexican childrenUlises de la Cruz-Mosso0José Francisco Muñoz-Valle1Aralia Berenice Salgado-Bernabé2Natividad Castro-Alarcón3Lorenzo Salgado-Goytia4José Sánchez-Corona5Silvia Esperanza Flores-Martínez6Isela Parra-Rojas7BSc, Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo, Guerrero, MéxicoDoutor, Departamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, MéxicoBSc, Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo, Guerrero, MéxicoDoutora, Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo, Guerrero, MéxicoDoutora, Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo, Guerrero, MéxicoDoutor, División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, MéxicoDoutor, División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, MéxicoDoutora, Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo, Guerrero, MéxicoObjective: To assess whether the -675 4G/5G polymorphism in the plasminogen activator inhibitor-1 gene is associated with obesity and insulin resistance in Mexican children. Methods: A cross-sectional study was performed in 174 children, 89 with normal-weight and 85 with obesity, aged from 6 to 13 years. All children were from state of Guerrero, and recruited from three primary schools in the city of Chilpancingo, Mexico. Insulin levels were determined by immunoenzymatic assay. The homeostasis model assessment was used to determine insulin resistance. The -675 4G/5G polymorphism in PAI-1 gene was analyzed by polymerase chain reaction-restriction fragment length polymorphism. Results: The prevalence of insulin resistance in the obese group was higher (49.41%) than in the normal-weight group (16.85%). The 4G/5G PAI-1 polymorphism was found in Hardy Weinberg equilibrium. The 4G/5G genotype contributed to a significant increase in waist-hip ratio (b = 0.02, p = 0.006), waist circumference (b = 4.42, p = 0.009), and subscapular skinfold thickness (b = 1.79, p = 0.04); however, it was not related with insulin resistance. Conclusion: The -675 4G/5G genotype of PAI-1 gene was associated with increase of body adiposity in Mexican children.http://www.sciencedirect.com/science/article/pii/S2255553613000955PAI-1 genePolymorphismsBody adiposityInsulin resistance
spellingShingle Ulises de la Cruz-Mosso
José Francisco Muñoz-Valle
Aralia Berenice Salgado-Bernabé
Natividad Castro-Alarcón
Lorenzo Salgado-Goytia
José Sánchez-Corona
Silvia Esperanza Flores-Martínez
Isela Parra-Rojas
Body adiposity but not insulin resistance is associated with -675 4G/5G polymorphism in the PAI-1 gene in a sample of Mexican children
Jornal de Pediatria (Versão em Português)
PAI-1 gene
Polymorphisms
Body adiposity
Insulin resistance
title Body adiposity but not insulin resistance is associated with -675 4G/5G polymorphism in the PAI-1 gene in a sample of Mexican children
title_full Body adiposity but not insulin resistance is associated with -675 4G/5G polymorphism in the PAI-1 gene in a sample of Mexican children
title_fullStr Body adiposity but not insulin resistance is associated with -675 4G/5G polymorphism in the PAI-1 gene in a sample of Mexican children
title_full_unstemmed Body adiposity but not insulin resistance is associated with -675 4G/5G polymorphism in the PAI-1 gene in a sample of Mexican children
title_short Body adiposity but not insulin resistance is associated with -675 4G/5G polymorphism in the PAI-1 gene in a sample of Mexican children
title_sort body adiposity but not insulin resistance is associated with 675 4g 5g polymorphism in the pai 1 gene in a sample of mexican children
topic PAI-1 gene
Polymorphisms
Body adiposity
Insulin resistance
url http://www.sciencedirect.com/science/article/pii/S2255553613000955
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