| Summary: | The aim of this study was to produce biscuit from blend of mushroom flour and wheat flour. The composite flour was at 0, 10, 20, 30 and 40% level of mushroom inclusion. The biscuit was analyzed for proximate composition, physical properties, mineral content, antioxidant properties, carbohydrate inhibitory enzyme activities, glycemic index, trend in blood glucose concentration, microbiological and sensory evaluation. Result from the proximate analysis showed that as the proportion of mushroom flour increased in the blend the protein con also increased. The protein content ranged from 10.36 to 17.92% while ash content ranged from 0.63 to 3.31%. Result from the physical properties of the biscuit showed that the spread ratio increased with increase in incorporation of the mushroom flour. The result from the mineral analysis revealed that sodium and calcium content increased with the increase in the level of the mushroom flour. Result from carbohydrate inhibitory enzyme activities showed that the biscuit inhibited key digestive enzymes such as (α- amylase and α- glucosidase). The result of glycemic index showed that the biscuit possessed low glycemic index and glycemic load. The trend in blood glucose concentration of rats fed with the biscuit showed that increasing level of the mushroom flour has a positive effect to lower the postprandial blood glucose response with WFMRB4 being the lowest. The microbial count ranged from 2 × 102 to 5.0 × 102 Cfu/g for bacteria count and 1.0 × 102 to 3.0 × 102 cfu/g for mold count. E coli was not detected in the mushroom biscuit. Sensory evaluation result showed that the biscuits were rated above average. The mushroom biscuit can therefore be used to manage diabetes mellitus and overcome protein-energy deficiency.
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