Identification and control for the effects of bioinformatic globin depletion on human RNA-seq differential expression analysis
Abstract When profiling blood samples by RNA-sequencing (RNA-seq), RNA from haemoglobin (Hgb) can account for up to 70% of the transcriptome. Due to considerations of sequencing depth and power to detect biological variation, Hgb RNA is typically depleted prior to sequencing by hybridisation-based m...
Main Authors: | Dylan Sheerin, Francisco Lakay, Hanif Esmail, Craig Kinnear, Bianca Sansom, Brigitte Glanzmann, Robert J. Wilkinson, Matthew E. Ritchie, Anna K. Coussens |
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Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2023-02-01
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Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-023-28218-7 |
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