<i>Microcystis aeruginosa</i> and <i>M. wesenbergii</i> Were the Primary Planktonic Microcystin Producers in Several Bulgarian Waterbodies (August 2019)
The rising interest in harmful cyanoprokaryote blooms promotes an increase of phycological and ecological research on potentially toxic species and their hazardous substances. The present study aimed to identify the main microcystin (MC) producers and their contribution to the phytoplankton of shall...
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MDPI AG
2020-12-01
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| Online Access: | https://www.mdpi.com/2076-3417/11/1/357 |
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| author | Maya Stoyneva-Gärtner Katerina Stefanova Jean-Pierre Descy Blagoy Uzunov Mariana Radkova Vera Pavlova Mariya Mitreva Georg Gärtner |
| author_facet | Maya Stoyneva-Gärtner Katerina Stefanova Jean-Pierre Descy Blagoy Uzunov Mariana Radkova Vera Pavlova Mariya Mitreva Georg Gärtner |
| author_sort | Maya Stoyneva-Gärtner |
| collection | DOAJ |
| description | The rising interest in harmful cyanoprokaryote blooms promotes an increase of phycological and ecological research on potentially toxic species and their hazardous substances. The present study aimed to identify the main microcystin (MC) producers and their contribution to the phytoplankton of shallow waterbodies in Bulgaria, applying different methods. The sampling was performed in August 2019 in nine lakes and reservoirs, two of which (reservoirs Kriva Reka and Izvornik 2) were studied for the first time. The high contribution of cyanoprokaryotes to the total species composition and phytoplankton abundance was proved by light microscopic (LM) observations and HPLC analysis of marker pigments. The LM identification of potential MC-producers was supported by PCR amplification of <i>mcyE</i> and <i>mcyB</i> genes. The MCs amounts, detected by HPLC-DAD, varied by sites with a range from undetectable concentrations to 0.46 µg L<sup>−1</sup> with only one recorded variant, namely MC-LR. It was found only in the reservoirs Mandra and Durankulak, while toxigenic MC-strains were obtained by PCR from five more waterbodies. Both LM and PCR demonstrated that the MC-producers were <i>Microcystis aeruginosa</i> and <i>M. wesenbergii</i>, despite their occurrence in low amounts (<0.5–5% of the total biomass) when filamentous cyanoprokaryotes dominated. |
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| language | English |
| last_indexed | 2024-03-10T13:34:47Z |
| publishDate | 2020-12-01 |
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| series | Applied Sciences |
| spelling | doaj.art-4c5a11ced2c047b7a4fe4c35fdfaacb82023-11-21T07:37:50ZengMDPI AGApplied Sciences2076-34172020-12-0111135710.3390/app11010357<i>Microcystis aeruginosa</i> and <i>M. wesenbergii</i> Were the Primary Planktonic Microcystin Producers in Several Bulgarian Waterbodies (August 2019)Maya Stoyneva-Gärtner0Katerina Stefanova1Jean-Pierre Descy2Blagoy Uzunov3Mariana Radkova4Vera Pavlova5Mariya Mitreva6Georg Gärtner7Faculty of Biology, Department of Botany, Sofia University, 8 Blvd. Dragan Zankov, 1164 Sofia, BulgariaAgroBioInstitute, Bulgarian Agricultural Academy, 8 Blvd. Dragan Zankov, 1164 Sofia, BulgariaUnité d’Océanographie Chimique, Université de Liège, Sart Tilman, 4000 Liège, BelgiumFaculty of Biology, Department of Botany, Sofia University, 8 Blvd. Dragan Zankov, 1164 Sofia, BulgariaAgroBioInstitute, Bulgarian Agricultural Academy, 8 Blvd. Dragan Zankov, 1164 Sofia, BulgariaNational Center of Public Health and Analyses, “I. Geshov” str. 15, 1431 Sofia, BulgariaNational Center of Public Health and Analyses, “I. Geshov” str. 15, 1431 Sofia, BulgariaInstitut für Botanik, Universität Innsbruck, Sternwartestrasse 15, 6020 Innsbruck, AustriaThe rising interest in harmful cyanoprokaryote blooms promotes an increase of phycological and ecological research on potentially toxic species and their hazardous substances. The present study aimed to identify the main microcystin (MC) producers and their contribution to the phytoplankton of shallow waterbodies in Bulgaria, applying different methods. The sampling was performed in August 2019 in nine lakes and reservoirs, two of which (reservoirs Kriva Reka and Izvornik 2) were studied for the first time. The high contribution of cyanoprokaryotes to the total species composition and phytoplankton abundance was proved by light microscopic (LM) observations and HPLC analysis of marker pigments. The LM identification of potential MC-producers was supported by PCR amplification of <i>mcyE</i> and <i>mcyB</i> genes. The MCs amounts, detected by HPLC-DAD, varied by sites with a range from undetectable concentrations to 0.46 µg L<sup>−1</sup> with only one recorded variant, namely MC-LR. It was found only in the reservoirs Mandra and Durankulak, while toxigenic MC-strains were obtained by PCR from five more waterbodies. Both LM and PCR demonstrated that the MC-producers were <i>Microcystis aeruginosa</i> and <i>M. wesenbergii</i>, despite their occurrence in low amounts (<0.5–5% of the total biomass) when filamentous cyanoprokaryotes dominated.https://www.mdpi.com/2076-3417/11/1/357coastal waterbodiescyanobacteriacyanoprokaryotescyanotoxinsharmful bloomslakes |
| spellingShingle | Maya Stoyneva-Gärtner Katerina Stefanova Jean-Pierre Descy Blagoy Uzunov Mariana Radkova Vera Pavlova Mariya Mitreva Georg Gärtner <i>Microcystis aeruginosa</i> and <i>M. wesenbergii</i> Were the Primary Planktonic Microcystin Producers in Several Bulgarian Waterbodies (August 2019) Applied Sciences coastal waterbodies cyanobacteria cyanoprokaryotes cyanotoxins harmful blooms lakes |
| title | <i>Microcystis aeruginosa</i> and <i>M. wesenbergii</i> Were the Primary Planktonic Microcystin Producers in Several Bulgarian Waterbodies (August 2019) |
| title_full | <i>Microcystis aeruginosa</i> and <i>M. wesenbergii</i> Were the Primary Planktonic Microcystin Producers in Several Bulgarian Waterbodies (August 2019) |
| title_fullStr | <i>Microcystis aeruginosa</i> and <i>M. wesenbergii</i> Were the Primary Planktonic Microcystin Producers in Several Bulgarian Waterbodies (August 2019) |
| title_full_unstemmed | <i>Microcystis aeruginosa</i> and <i>M. wesenbergii</i> Were the Primary Planktonic Microcystin Producers in Several Bulgarian Waterbodies (August 2019) |
| title_short | <i>Microcystis aeruginosa</i> and <i>M. wesenbergii</i> Were the Primary Planktonic Microcystin Producers in Several Bulgarian Waterbodies (August 2019) |
| title_sort | i microcystis aeruginosa i and i m wesenbergii i were the primary planktonic microcystin producers in several bulgarian waterbodies august 2019 |
| topic | coastal waterbodies cyanobacteria cyanoprokaryotes cyanotoxins harmful blooms lakes |
| url | https://www.mdpi.com/2076-3417/11/1/357 |
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