Detection of Drug Resistant Mycobacterium Tuberculosis Strains Using Kit SIRE Nitratase®: a Multicenter Study
Abstract (1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2020-07-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132020000100308&tlng=en |
| _version_ | 1798021500568076288 |
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| author | Silvana Spindola de Miranda Isabela Neves de Almeida Maria de Fátima Filardi Oliveira Mansur Lida Jouca de Assis Figueredo Wânia da Silva Carvalho João Paulo Amaral Hadaad Jaciara de Lourdes do Carmo Guimarães Diniz Andrea von Groll Pedro Almeida da Silva Maria Luiza Lopes Marcelo Cordeiro dos Santos Alexandra Brito Fernanda Carvalho de Queiroz Mello Thiago da Silva Santos Malaquias Julio Croda Juliana Maira Watanabe Pinhata Rosângela Siqueira de Oliveira Erica Chimara Maria Lúcia Rossetti Maria Laura Halon Maria Cristina Lourenço Reginalda Ferreira de Melo Medeiros Fátima Cristina Onofre Fandinho Montes Diana Machado Miguel Viveiros Afrânio Lineu Kritski |
| author_facet | Silvana Spindola de Miranda Isabela Neves de Almeida Maria de Fátima Filardi Oliveira Mansur Lida Jouca de Assis Figueredo Wânia da Silva Carvalho João Paulo Amaral Hadaad Jaciara de Lourdes do Carmo Guimarães Diniz Andrea von Groll Pedro Almeida da Silva Maria Luiza Lopes Marcelo Cordeiro dos Santos Alexandra Brito Fernanda Carvalho de Queiroz Mello Thiago da Silva Santos Malaquias Julio Croda Juliana Maira Watanabe Pinhata Rosângela Siqueira de Oliveira Erica Chimara Maria Lúcia Rossetti Maria Laura Halon Maria Cristina Lourenço Reginalda Ferreira de Melo Medeiros Fátima Cristina Onofre Fandinho Montes Diana Machado Miguel Viveiros Afrânio Lineu Kritski |
| author_sort | Silvana Spindola de Miranda |
| collection | DOAJ |
| description | Abstract (1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries. |
| first_indexed | 2024-04-11T17:14:38Z |
| format | Article |
| id | doaj.art-4c5c040d80b14a3981af4b9f0255a4af |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-04-11T17:14:38Z |
| publishDate | 2020-07-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-4c5c040d80b14a3981af4b9f0255a4af2022-12-22T04:12:44ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242020-07-016310.1590/1678-4324-2020190179Detection of Drug Resistant Mycobacterium Tuberculosis Strains Using Kit SIRE Nitratase®: a Multicenter StudySilvana Spindola de Mirandahttps://orcid.org/0000-0001-7245-4472Isabela Neves de Almeidahttps://orcid.org/0000-0001-6152-7648Maria de Fátima Filardi Oliveira Mansurhttps://orcid.org/0000-0002-2414-0273Lida Jouca de Assis Figueredohttps://orcid.org/0000-0001-5355-0784Wânia da Silva Carvalhohttps://orcid.org/0000-0002-2575-6352João Paulo Amaral Hadaadhttps://orcid.org/0000-0003-2823-6288Jaciara de Lourdes do Carmo Guimarães Dinizhttps://orcid.org/0000-0001-6210-5658Andrea von Grollhttps://orcid.org/0000-0002-6727-372XPedro Almeida da Silvahttps://orcid.org/0000-0003-1666-1295Maria Luiza Lopeshttps://orcid.org/0000-0001-6894-1366Marcelo Cordeiro dos Santoshttps://orcid.org/0000-0002-7140-7145Alexandra Britohttps://orcid.org/0000-0002-9036-702XFernanda Carvalho de Queiroz Mellohttps://orcid.org/0000-0003-3250-6738Thiago da Silva Santos Malaquiashttps://orcid.org/0000-0001-8783-8962Julio Crodahttps://orcid.org/0000-0002-6665-6825Juliana Maira Watanabe Pinhatahttps://orcid.org/0000-0001-5758-6688Rosângela Siqueira de Oliveirahttps://orcid.org/0000-0002-5188-8367Erica Chimarahttps://orcid.org/0000-0001-9574-8449Maria Lúcia Rossettihttps://orcid.org/0000-0002-9672-9394Maria Laura Halonhttps://orcid.org/0000-0001-5866-0827Maria Cristina Lourençohttps://orcid.org/0000-0003-0382-9108Reginalda Ferreira de Melo Medeiroshttps://orcid.org/0000-0001-9357-4932Fátima Cristina Onofre Fandinho Monteshttps://orcid.org/0000-0003-3896-3526Diana Machadohttps://orcid.org/0000-0002-6740-2632Miguel Viveiroshttps://orcid.org/0000-0001-9676-6251Afrânio Lineu Kritskihttps://orcid.org/0000-0002-5900-6007Abstract (1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132020000100308&tlng=entuberculosisMDRnitrate reductasetechnology |
| spellingShingle | Silvana Spindola de Miranda Isabela Neves de Almeida Maria de Fátima Filardi Oliveira Mansur Lida Jouca de Assis Figueredo Wânia da Silva Carvalho João Paulo Amaral Hadaad Jaciara de Lourdes do Carmo Guimarães Diniz Andrea von Groll Pedro Almeida da Silva Maria Luiza Lopes Marcelo Cordeiro dos Santos Alexandra Brito Fernanda Carvalho de Queiroz Mello Thiago da Silva Santos Malaquias Julio Croda Juliana Maira Watanabe Pinhata Rosângela Siqueira de Oliveira Erica Chimara Maria Lúcia Rossetti Maria Laura Halon Maria Cristina Lourenço Reginalda Ferreira de Melo Medeiros Fátima Cristina Onofre Fandinho Montes Diana Machado Miguel Viveiros Afrânio Lineu Kritski Detection of Drug Resistant Mycobacterium Tuberculosis Strains Using Kit SIRE Nitratase®: a Multicenter Study Brazilian Archives of Biology and Technology tuberculosis MDR nitrate reductase technology |
| title | Detection of Drug Resistant Mycobacterium Tuberculosis Strains Using Kit SIRE Nitratase®: a Multicenter Study |
| title_full | Detection of Drug Resistant Mycobacterium Tuberculosis Strains Using Kit SIRE Nitratase®: a Multicenter Study |
| title_fullStr | Detection of Drug Resistant Mycobacterium Tuberculosis Strains Using Kit SIRE Nitratase®: a Multicenter Study |
| title_full_unstemmed | Detection of Drug Resistant Mycobacterium Tuberculosis Strains Using Kit SIRE Nitratase®: a Multicenter Study |
| title_short | Detection of Drug Resistant Mycobacterium Tuberculosis Strains Using Kit SIRE Nitratase®: a Multicenter Study |
| title_sort | detection of drug resistant mycobacterium tuberculosis strains using kit sire nitratase r a multicenter study |
| topic | tuberculosis MDR nitrate reductase technology |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132020000100308&tlng=en |
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