Seasonality of sand flies (Diptera: Psychodidae) and Leishmania DNA detection in vector species in an area with endemic visceral leishmaniasis
BACKGROUND Leishmaniases are a serious health problem in southeast Brazil, including the city of Belo Horizonte (BH), Minas Gerais state (MG), where there are high rates of incidence and mortality due to visceral leishmaniases. BH is divided into nine sanitary districts (SD) of which one, the Venda...
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Fundação Oswaldo Cruz (FIOCRUZ)
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| author | Lara Saraiva Camila Gonçalves Leite Ana Cristina Vianna Mariano da Rocha Lima Luiz Otávio Alves de Carvalho Agnes Antônia Sampaio Pereira Jerônimo Marteleto Nunes Rugani Felipe Dutra Rego Célia Maria Ferreira Gontijo José Dilermando Andrade Filho |
| author_facet | Lara Saraiva Camila Gonçalves Leite Ana Cristina Vianna Mariano da Rocha Lima Luiz Otávio Alves de Carvalho Agnes Antônia Sampaio Pereira Jerônimo Marteleto Nunes Rugani Felipe Dutra Rego Célia Maria Ferreira Gontijo José Dilermando Andrade Filho |
| author_sort | Lara Saraiva |
| collection | DOAJ |
| description | BACKGROUND Leishmaniases are a serious health problem in southeast Brazil, including the city of Belo Horizonte (BH), Minas Gerais state (MG), where there are high rates of incidence and mortality due to visceral leishmaniases. BH is divided into nine sanitary districts (SD) of which one, the Venda Nova SD, was selected for this study because it has high rates of positivity for canine leishmaniasis and high incidence of human leishmaniasis. OBJECTIVES This study aimed to survey the sand fly fauna in Venda Nova SD from August 2011 to July 2013 and perform a descriptive analysis of the vector population. METHODS The sampling was carried out using automatic HP light traps at all covered areas of the Venda Nova SD, in a total of eighteen light traps. Sampled specimens were identified following Galati (2003), and females were submitted to molecular techniques for the detection and identification of Leishmania DNA. A simple environmental description was done for it area and Kernel estimation was used to infer vector density for each study site. FINDINGS A total of 2,427 sand fly specimens belonging to eight species and five genera were collected of which 95.3% were Lutzomyia longipalpis. The seasonal variation curve was delineated by this species. Lu. longipalpis was the most abundant at all collection points and in all months of the study, and exhibited a natural infection rate of 1.01% for Leishmania infantum and 1.77% for Leishmania braziliensis. MAIN CONCLUSIONS The results show the presence and adaptation of Lu. longipalpis to the anthropic environment of BH and reinforces its role as the main vector of L. infantum in the region. |
| first_indexed | 2024-03-12T18:07:18Z |
| format | Article |
| id | doaj.art-4c5d2236deca4337b70abbf877e1f26b |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:07:18Z |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-4c5d2236deca4337b70abbf877e1f26b2023-08-02T09:25:58ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-8060112430931810.1590/0074-02760160438S0074-02762017000400309Seasonality of sand flies (Diptera: Psychodidae) and Leishmania DNA detection in vector species in an area with endemic visceral leishmaniasisLara SaraivaCamila Gonçalves LeiteAna Cristina Vianna Mariano da Rocha LimaLuiz Otávio Alves de CarvalhoAgnes Antônia Sampaio PereiraJerônimo Marteleto Nunes RuganiFelipe Dutra RegoCélia Maria Ferreira GontijoJosé Dilermando Andrade FilhoBACKGROUND Leishmaniases are a serious health problem in southeast Brazil, including the city of Belo Horizonte (BH), Minas Gerais state (MG), where there are high rates of incidence and mortality due to visceral leishmaniases. BH is divided into nine sanitary districts (SD) of which one, the Venda Nova SD, was selected for this study because it has high rates of positivity for canine leishmaniasis and high incidence of human leishmaniasis. OBJECTIVES This study aimed to survey the sand fly fauna in Venda Nova SD from August 2011 to July 2013 and perform a descriptive analysis of the vector population. METHODS The sampling was carried out using automatic HP light traps at all covered areas of the Venda Nova SD, in a total of eighteen light traps. Sampled specimens were identified following Galati (2003), and females were submitted to molecular techniques for the detection and identification of Leishmania DNA. A simple environmental description was done for it area and Kernel estimation was used to infer vector density for each study site. FINDINGS A total of 2,427 sand fly specimens belonging to eight species and five genera were collected of which 95.3% were Lutzomyia longipalpis. The seasonal variation curve was delineated by this species. Lu. longipalpis was the most abundant at all collection points and in all months of the study, and exhibited a natural infection rate of 1.01% for Leishmania infantum and 1.77% for Leishmania braziliensis. MAIN CONCLUSIONS The results show the presence and adaptation of Lu. longipalpis to the anthropic environment of BH and reinforces its role as the main vector of L. infantum in the region.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017000400309&lng=en&tlng=envisceral leishmaniasisLutzomyia longipalpisBelo Horizonte |
| spellingShingle | Lara Saraiva Camila Gonçalves Leite Ana Cristina Vianna Mariano da Rocha Lima Luiz Otávio Alves de Carvalho Agnes Antônia Sampaio Pereira Jerônimo Marteleto Nunes Rugani Felipe Dutra Rego Célia Maria Ferreira Gontijo José Dilermando Andrade Filho Seasonality of sand flies (Diptera: Psychodidae) and Leishmania DNA detection in vector species in an area with endemic visceral leishmaniasis Memorias do Instituto Oswaldo Cruz visceral leishmaniasis Lutzomyia longipalpis Belo Horizonte |
| title | Seasonality of sand flies (Diptera: Psychodidae) and Leishmania DNA detection in vector species in an area with endemic visceral leishmaniasis |
| title_full | Seasonality of sand flies (Diptera: Psychodidae) and Leishmania DNA detection in vector species in an area with endemic visceral leishmaniasis |
| title_fullStr | Seasonality of sand flies (Diptera: Psychodidae) and Leishmania DNA detection in vector species in an area with endemic visceral leishmaniasis |
| title_full_unstemmed | Seasonality of sand flies (Diptera: Psychodidae) and Leishmania DNA detection in vector species in an area with endemic visceral leishmaniasis |
| title_short | Seasonality of sand flies (Diptera: Psychodidae) and Leishmania DNA detection in vector species in an area with endemic visceral leishmaniasis |
| title_sort | seasonality of sand flies diptera psychodidae and leishmania dna detection in vector species in an area with endemic visceral leishmaniasis |
| topic | visceral leishmaniasis Lutzomyia longipalpis Belo Horizonte |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017000400309&lng=en&tlng=en |
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