Immunohistochemistry of Programmed Cell Death in Archival Human Pathology Specimens
Immunohistochemistry (IHC) for detecting key signal molecules involved in programmed cell death (PCD) in archival human pathology specimens is fairly well established. Detection of cleaved caspase-3 in lymphocytes in rheumatoid arthritis (RA) and gastric surface foveolar glandular epithelia but not...
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MDPI AG
2012-05-01
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| author | Takami Matsuyama Yoshito Eizuru Takuro Kanekura Yoshifumi Kawano Shuji Izumo Xinshan Jia Katsuyuki Aozasa Taku Nagai Jia Wang Kazuhisa Hasui |
| author_facet | Takami Matsuyama Yoshito Eizuru Takuro Kanekura Yoshifumi Kawano Shuji Izumo Xinshan Jia Katsuyuki Aozasa Taku Nagai Jia Wang Kazuhisa Hasui |
| author_sort | Takami Matsuyama |
| collection | DOAJ |
| description | Immunohistochemistry (IHC) for detecting key signal molecules involved in programmed cell death (PCD) in archival human pathology specimens is fairly well established. Detection of cleaved caspase-3 in lymphocytes in rheumatoid arthritis (RA) and gastric surface foveolar glandular epithelia but not in synoviocytes in RA, gastric fundic glandular epithelia, or nasal NK/T-cell lymphoma (NKTCL) cells suggests anti-apoptotic mechanisms in cell differentiation and in oncogenesis such as the induction of survivin. Enzymatically pretreated and ultra-super sensitive detection of beclin-1 in synoviocytes in RA and gastric fundic glandular epithelia suggests enhanced autophagy. The deposition of beclin-1 in fibrinoid necrosis in RA and expression of beclin-1 in detached gastric fundic glandular cells suggest that enhanced autophagy undergoes autophagic cell death (ACD). NKTCL exhibited enhanced autophagy through LC3 labeling and showed densely LC3 labeled cell-debris in regions of peculiar necrosis without deposition of beclin-1, indicating massive ACD in NKTCL and the alternative pathway enhancing autophagy following autophagic vesicle nucleation. Autophagy progression was monitored by labeling aggregated mitochondria and cathepsin D. The cell-debris in massive ACD in NKTCL were positive for 8-hydroxydeoxyguanosine, suggesting DNA oxidation occurred in ACD. Immunohistochemical autophagy and PCD analysis in archival human pathology specimens may offer new insights into autophagy in humans. |
| first_indexed | 2024-03-12T05:41:25Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T05:41:25Z |
| publishDate | 2012-05-01 |
| publisher | MDPI AG |
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| series | Cells |
| spelling | doaj.art-4c61dcf5238d4fc98ca65ef2b75470412023-09-03T05:57:25ZengMDPI AGCells2073-44092012-05-0112748810.3390/cells1020074Immunohistochemistry of Programmed Cell Death in Archival Human Pathology SpecimensTakami MatsuyamaYoshito EizuruTakuro KanekuraYoshifumi KawanoShuji IzumoXinshan JiaKatsuyuki AozasaTaku NagaiJia WangKazuhisa HasuiImmunohistochemistry (IHC) for detecting key signal molecules involved in programmed cell death (PCD) in archival human pathology specimens is fairly well established. Detection of cleaved caspase-3 in lymphocytes in rheumatoid arthritis (RA) and gastric surface foveolar glandular epithelia but not in synoviocytes in RA, gastric fundic glandular epithelia, or nasal NK/T-cell lymphoma (NKTCL) cells suggests anti-apoptotic mechanisms in cell differentiation and in oncogenesis such as the induction of survivin. Enzymatically pretreated and ultra-super sensitive detection of beclin-1 in synoviocytes in RA and gastric fundic glandular epithelia suggests enhanced autophagy. The deposition of beclin-1 in fibrinoid necrosis in RA and expression of beclin-1 in detached gastric fundic glandular cells suggest that enhanced autophagy undergoes autophagic cell death (ACD). NKTCL exhibited enhanced autophagy through LC3 labeling and showed densely LC3 labeled cell-debris in regions of peculiar necrosis without deposition of beclin-1, indicating massive ACD in NKTCL and the alternative pathway enhancing autophagy following autophagic vesicle nucleation. Autophagy progression was monitored by labeling aggregated mitochondria and cathepsin D. The cell-debris in massive ACD in NKTCL were positive for 8-hydroxydeoxyguanosine, suggesting DNA oxidation occurred in ACD. Immunohistochemical autophagy and PCD analysis in archival human pathology specimens may offer new insights into autophagy in humans.http://www.mdpi.com/2073-4409/1/2/74antigen retrieval and immunohistochemistryprogrammed cell deathapoptosisautophagyarchival formalin-fixed and paraffin-embedded human pathology specimen |
| spellingShingle | Takami Matsuyama Yoshito Eizuru Takuro Kanekura Yoshifumi Kawano Shuji Izumo Xinshan Jia Katsuyuki Aozasa Taku Nagai Jia Wang Kazuhisa Hasui Immunohistochemistry of Programmed Cell Death in Archival Human Pathology Specimens Cells antigen retrieval and immunohistochemistry programmed cell death apoptosis autophagy archival formalin-fixed and paraffin-embedded human pathology specimen |
| title | Immunohistochemistry of Programmed Cell Death in Archival Human Pathology Specimens |
| title_full | Immunohistochemistry of Programmed Cell Death in Archival Human Pathology Specimens |
| title_fullStr | Immunohistochemistry of Programmed Cell Death in Archival Human Pathology Specimens |
| title_full_unstemmed | Immunohistochemistry of Programmed Cell Death in Archival Human Pathology Specimens |
| title_short | Immunohistochemistry of Programmed Cell Death in Archival Human Pathology Specimens |
| title_sort | immunohistochemistry of programmed cell death in archival human pathology specimens |
| topic | antigen retrieval and immunohistochemistry programmed cell death apoptosis autophagy archival formalin-fixed and paraffin-embedded human pathology specimen |
| url | http://www.mdpi.com/2073-4409/1/2/74 |
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