In Vitro Shoot Bud Differentiation from Hypocotyl Explants of Chili Peppers (Capsicum annuum L.)

Chili pepper (Capsicum annuum L.) is an economically important spice crop in tropical and subtropical countries. In vitro plant regeneration was obtained from 15th day old hypocotyl explants of three chili pepper cultivars (Capsicum annuum L., var. �X-235�, var. �PC-1� and var. �Pusa Jwala�). Among...

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Main Authors: Owk ANIEL KUMAR, Sape SUBBA TATA
Format: Article
Language:English
Published: Society of Land Measurements and Cadastre from Transylvania (SMTCT) 2010-03-01
Series:Notulae Scientia Biologicae
Online Access:http://notulaebiologicae.ro/index.php/nsb/article/view/3553
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author Owk ANIEL KUMAR
Sape SUBBA TATA
author_facet Owk ANIEL KUMAR
Sape SUBBA TATA
author_sort Owk ANIEL KUMAR
collection DOAJ
description Chili pepper (Capsicum annuum L.) is an economically important spice crop in tropical and subtropical countries. In vitro plant regeneration was obtained from 15th day old hypocotyl explants of three chili pepper cultivars (Capsicum annuum L., var. �X-235�, var. �PC-1� and var. �Pusa Jwala�). Among the genotypes of Capsicum L. var. �X-235� responded better than the var. �PC-1� and var. �Pusa Jwala�. MS medium containing BAP (4.0 mg/l) and IAA (0.5 mg/l) was found to be the best medium for the production of maximum number of shoot buds in all the genotypes of chili pepper i.e., 6.80�0.16 (var. �X-235�), 5.00�0.19 (var. �PC-1�) and 4.80�0.12 (var. �Pusa Jwala�). The shoots were rooted on MS medium fortified with IBA (0.5 mg/l). Rooted plants were hardened and transplanted to the soil. The plants showed 80-90% survival during transplantation.
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spelling doaj.art-4c63dadb8e8b4579a2ed90a73ba912b22022-12-22T00:50:36ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32052067-32642010-03-012166694821In Vitro Shoot Bud Differentiation from Hypocotyl Explants of Chili Peppers (Capsicum annuum L.)Owk ANIEL KUMAR0Sape SUBBA TATA1Andhra University, Department of Botany, VisakhapatnamAndhra University, Department of Botany, VisakhapatnamChili pepper (Capsicum annuum L.) is an economically important spice crop in tropical and subtropical countries. In vitro plant regeneration was obtained from 15th day old hypocotyl explants of three chili pepper cultivars (Capsicum annuum L., var. �X-235�, var. �PC-1� and var. �Pusa Jwala�). Among the genotypes of Capsicum L. var. �X-235� responded better than the var. �PC-1� and var. �Pusa Jwala�. MS medium containing BAP (4.0 mg/l) and IAA (0.5 mg/l) was found to be the best medium for the production of maximum number of shoot buds in all the genotypes of chili pepper i.e., 6.80�0.16 (var. �X-235�), 5.00�0.19 (var. �PC-1�) and 4.80�0.12 (var. �Pusa Jwala�). The shoots were rooted on MS medium fortified with IBA (0.5 mg/l). Rooted plants were hardened and transplanted to the soil. The plants showed 80-90% survival during transplantation.http://notulaebiologicae.ro/index.php/nsb/article/view/3553
spellingShingle Owk ANIEL KUMAR
Sape SUBBA TATA
In Vitro Shoot Bud Differentiation from Hypocotyl Explants of Chili Peppers (Capsicum annuum L.)
Notulae Scientia Biologicae
title In Vitro Shoot Bud Differentiation from Hypocotyl Explants of Chili Peppers (Capsicum annuum L.)
title_full In Vitro Shoot Bud Differentiation from Hypocotyl Explants of Chili Peppers (Capsicum annuum L.)
title_fullStr In Vitro Shoot Bud Differentiation from Hypocotyl Explants of Chili Peppers (Capsicum annuum L.)
title_full_unstemmed In Vitro Shoot Bud Differentiation from Hypocotyl Explants of Chili Peppers (Capsicum annuum L.)
title_short In Vitro Shoot Bud Differentiation from Hypocotyl Explants of Chili Peppers (Capsicum annuum L.)
title_sort in vitro shoot bud differentiation from hypocotyl explants of chili peppers capsicum annuum l
url http://notulaebiologicae.ro/index.php/nsb/article/view/3553
work_keys_str_mv AT owkanielkumar invitroshootbuddifferentiationfromhypocotylexplantsofchilipepperscapsicumannuuml
AT sapesubbatata invitroshootbuddifferentiationfromhypocotylexplantsofchilipepperscapsicumannuuml