Resistance to tomato yellow leaf curl virus-Thailand isolate (TYLCTHV-[2]) and markers loci association in BC2F1 population from a cross between Seedathip 3 and a wild tomato, Solanum habrochaites ‘L06112’ clone no.1
The BC2F1 population from a cross between wild tomato, Solanum habrochaites ‘L06112’ and recurrent susceptiblevariety, Seedathip 3 was investigated for a resistance to Tomato yellow leaf curl Thailand virus (TYLCTHV-[2]). Five stemcuttings from each of the 196 lines were inoculated with TYLCTHV-[2]...
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2012-02-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | http://rdo.psu.ac.th/sjstweb/journal/34-1/0125-3395-34-1-31-36.pdf |
| _version_ | 1811234672875667456 |
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| author | Uraiwan Pongpayaklers Ornubol Chomdej Julapark Chunwongse |
| author_facet | Uraiwan Pongpayaklers Ornubol Chomdej Julapark Chunwongse |
| author_sort | Uraiwan Pongpayaklers |
| collection | DOAJ |
| description | The BC2F1 population from a cross between wild tomato, Solanum habrochaites ‘L06112’ and recurrent susceptiblevariety, Seedathip 3 was investigated for a resistance to Tomato yellow leaf curl Thailand virus (TYLCTHV-[2]). Five stemcuttings from each of the 196 lines were inoculated with TYLCTHV-[2] using viruliferous whiteflies as the inoculation vector.Disease response was recorded weekly intervals and scored for three weeks according to the severity of the symptoms. Thepresence of TYLCTHV-[2] was confirmed by enzyme-linked immunosorbent assay (ELISA) at three weeks after inoculation.ELISA readings showed a normal distribution for the BC2F1 population ranging from 0.055 (resistant) to 0.930 (susceptible).DNA samples from BC2F1 population were analyzed for genes and markers association; Ty-2 on chromosome 11 using AVRDCprimer number 11-090.0 (TG105A), and Ty-3 on chromosome 6 using marker C2_At3g11210. Results showed that Ty-2 waslost during stepwise selection at BC1F1 generation, while Ty-3 showed no relationship to marker C2_At3g11210 and theamount of virus detected from ELISA reading. This indicated that the TYLCTHV-[2] resistant phenotype response in theBC2F1 population neither came from Ty-2 nor linked to the Ty-3 gene. |
| first_indexed | 2024-04-12T11:40:39Z |
| format | Article |
| id | doaj.art-4c654b7c0ab94990ae4ebf73cd4ba99c |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-04-12T11:40:39Z |
| publishDate | 2012-02-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-4c654b7c0ab94990ae4ebf73cd4ba99c2022-12-22T03:34:40ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952012-02-013413136Resistance to tomato yellow leaf curl virus-Thailand isolate (TYLCTHV-[2]) and markers loci association in BC2F1 population from a cross between Seedathip 3 and a wild tomato, Solanum habrochaites ‘L06112’ clone no.1Uraiwan PongpayaklersOrnubol ChomdejJulapark ChunwongseThe BC2F1 population from a cross between wild tomato, Solanum habrochaites ‘L06112’ and recurrent susceptiblevariety, Seedathip 3 was investigated for a resistance to Tomato yellow leaf curl Thailand virus (TYLCTHV-[2]). Five stemcuttings from each of the 196 lines were inoculated with TYLCTHV-[2] using viruliferous whiteflies as the inoculation vector.Disease response was recorded weekly intervals and scored for three weeks according to the severity of the symptoms. Thepresence of TYLCTHV-[2] was confirmed by enzyme-linked immunosorbent assay (ELISA) at three weeks after inoculation.ELISA readings showed a normal distribution for the BC2F1 population ranging from 0.055 (resistant) to 0.930 (susceptible).DNA samples from BC2F1 population were analyzed for genes and markers association; Ty-2 on chromosome 11 using AVRDCprimer number 11-090.0 (TG105A), and Ty-3 on chromosome 6 using marker C2_At3g11210. Results showed that Ty-2 waslost during stepwise selection at BC1F1 generation, while Ty-3 showed no relationship to marker C2_At3g11210 and theamount of virus detected from ELISA reading. This indicated that the TYLCTHV-[2] resistant phenotype response in theBC2F1 population neither came from Ty-2 nor linked to the Ty-3 gene.http://rdo.psu.ac.th/sjstweb/journal/34-1/0125-3395-34-1-31-36.pdfbreedingdisease resistanceTYLCVTYLCTHV-[2]wild tomato introgressionSolanum habrochaites |
| spellingShingle | Uraiwan Pongpayaklers Ornubol Chomdej Julapark Chunwongse Resistance to tomato yellow leaf curl virus-Thailand isolate (TYLCTHV-[2]) and markers loci association in BC2F1 population from a cross between Seedathip 3 and a wild tomato, Solanum habrochaites ‘L06112’ clone no.1 Songklanakarin Journal of Science and Technology (SJST) breeding disease resistance TYLCV TYLCTHV-[2] wild tomato introgression Solanum habrochaites |
| title | Resistance to tomato yellow leaf curl virus-Thailand isolate (TYLCTHV-[2]) and markers loci association in BC2F1 population from a cross between Seedathip 3 and a wild tomato, Solanum habrochaites ‘L06112’ clone no.1 |
| title_full | Resistance to tomato yellow leaf curl virus-Thailand isolate (TYLCTHV-[2]) and markers loci association in BC2F1 population from a cross between Seedathip 3 and a wild tomato, Solanum habrochaites ‘L06112’ clone no.1 |
| title_fullStr | Resistance to tomato yellow leaf curl virus-Thailand isolate (TYLCTHV-[2]) and markers loci association in BC2F1 population from a cross between Seedathip 3 and a wild tomato, Solanum habrochaites ‘L06112’ clone no.1 |
| title_full_unstemmed | Resistance to tomato yellow leaf curl virus-Thailand isolate (TYLCTHV-[2]) and markers loci association in BC2F1 population from a cross between Seedathip 3 and a wild tomato, Solanum habrochaites ‘L06112’ clone no.1 |
| title_short | Resistance to tomato yellow leaf curl virus-Thailand isolate (TYLCTHV-[2]) and markers loci association in BC2F1 population from a cross between Seedathip 3 and a wild tomato, Solanum habrochaites ‘L06112’ clone no.1 |
| title_sort | resistance to tomato yellow leaf curl virus thailand isolate tylcthv 2 and markers loci association in bc2f1 population from a cross between seedathip 3 and a wild tomato solanum habrochaites l06112 clone no 1 |
| topic | breeding disease resistance TYLCV TYLCTHV-[2] wild tomato introgression Solanum habrochaites |
| url | http://rdo.psu.ac.th/sjstweb/journal/34-1/0125-3395-34-1-31-36.pdf |
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