Regulation of Expression of Autophagy Genes by Atg8a-Interacting Partners Sequoia, YL-1, and Sir2 in Drosophila
Summary: Autophagy is the degradation of cytoplasmic material through the lysosomal pathway. One of the most studied autophagy-related proteins is LC3. Despite growing evidence that LC3 is enriched in the nucleus, its nuclear role is poorly understood. Here, we show that Drosophila Atg8a protein, ho...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-05-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124720306483 |
| _version_ | 1818515061201698816 |
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| author | Anne-Claire Jacomin Stavroula Petridi Marisa Di Monaco Zambarlal Bhujabal Ashish Jain Nitha C. Mulakkal Anthimi Palara Emma L. Powell Bonita Chung Cleidiane Zampronio Alexandra Jones Alexander Cameron Terje Johansen Ioannis P. Nezis |
| author_facet | Anne-Claire Jacomin Stavroula Petridi Marisa Di Monaco Zambarlal Bhujabal Ashish Jain Nitha C. Mulakkal Anthimi Palara Emma L. Powell Bonita Chung Cleidiane Zampronio Alexandra Jones Alexander Cameron Terje Johansen Ioannis P. Nezis |
| author_sort | Anne-Claire Jacomin |
| collection | DOAJ |
| description | Summary: Autophagy is the degradation of cytoplasmic material through the lysosomal pathway. One of the most studied autophagy-related proteins is LC3. Despite growing evidence that LC3 is enriched in the nucleus, its nuclear role is poorly understood. Here, we show that Drosophila Atg8a protein, homologous to mammalian LC3, interacts with the transcription factor Sequoia in a LIR motif-dependent manner. We show that Sequoia depletion induces autophagy in nutrient-rich conditions through the enhanced expression of autophagy genes. We show that Atg8a interacts with YL-1, a component of a nuclear acetyltransferase complex, and that it is acetylated in nutrient-rich conditions. We also show that Atg8a interacts with the deacetylase Sir2, which deacetylates Atg8a during starvation to activate autophagy. Our results suggest a mechanism of regulation of the expression of autophagy genes by Atg8a, which is linked to its acetylation status and its interaction with Sequoia, YL-1, and Sir2. |
| first_indexed | 2024-12-11T00:24:06Z |
| format | Article |
| id | doaj.art-4c659952edc443cfb7875849b87d2315 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-11T00:24:06Z |
| publishDate | 2020-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-4c659952edc443cfb7875849b87d23152022-12-22T01:27:37ZengElsevierCell Reports2211-12472020-05-01318Regulation of Expression of Autophagy Genes by Atg8a-Interacting Partners Sequoia, YL-1, and Sir2 in DrosophilaAnne-Claire Jacomin0Stavroula Petridi1Marisa Di Monaco2Zambarlal Bhujabal3Ashish Jain4Nitha C. Mulakkal5Anthimi Palara6Emma L. Powell7Bonita Chung8Cleidiane Zampronio9Alexandra Jones10Alexander Cameron11Terje Johansen12Ioannis P. Nezis13School of Life Sciences, University of Warwick, CV4 7AL Coventry, UKSchool of Life Sciences, University of Warwick, CV4 7AL Coventry, UKSchool of Life Sciences, University of Warwick, CV4 7AL Coventry, UKMolecular Cancer Research Group, Institute of Medical Biology, University of Tromsø–The Arctic University of Norway, 9037 Tromsø, NorwayMolecular Cancer Research Group, Institute of Medical Biology, University of Tromsø–The Arctic University of Norway, 9037 Tromsø, Norway; Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, Montebello, 0379 Oslo, Norway; Centre for Cancer Cell Reprogramming, Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Montebello, 0379 Oslo, NorwaySchool of Life Sciences, University of Warwick, CV4 7AL Coventry, UKSchool of Life Sciences, University of Warwick, CV4 7AL Coventry, UK; Molecular Cancer Research Group, Institute of Medical Biology, University of Tromsø–The Arctic University of Norway, 9037 Tromsø, NorwaySchool of Life Sciences, University of Warwick, CV4 7AL Coventry, UKSchool of Life Sciences, University of Warwick, CV4 7AL Coventry, UKSchool of Life Sciences, University of Warwick, CV4 7AL Coventry, UKSchool of Life Sciences, University of Warwick, CV4 7AL Coventry, UKSchool of Life Sciences, University of Warwick, CV4 7AL Coventry, UKMolecular Cancer Research Group, Institute of Medical Biology, University of Tromsø–The Arctic University of Norway, 9037 Tromsø, NorwaySchool of Life Sciences, University of Warwick, CV4 7AL Coventry, UK; Corresponding authorSummary: Autophagy is the degradation of cytoplasmic material through the lysosomal pathway. One of the most studied autophagy-related proteins is LC3. Despite growing evidence that LC3 is enriched in the nucleus, its nuclear role is poorly understood. Here, we show that Drosophila Atg8a protein, homologous to mammalian LC3, interacts with the transcription factor Sequoia in a LIR motif-dependent manner. We show that Sequoia depletion induces autophagy in nutrient-rich conditions through the enhanced expression of autophagy genes. We show that Atg8a interacts with YL-1, a component of a nuclear acetyltransferase complex, and that it is acetylated in nutrient-rich conditions. We also show that Atg8a interacts with the deacetylase Sir2, which deacetylates Atg8a during starvation to activate autophagy. Our results suggest a mechanism of regulation of the expression of autophagy genes by Atg8a, which is linked to its acetylation status and its interaction with Sequoia, YL-1, and Sir2.http://www.sciencedirect.com/science/article/pii/S2211124720306483acetylationautophagyLIR motifnucleustranscriptionLC3/Atg8 |
| spellingShingle | Anne-Claire Jacomin Stavroula Petridi Marisa Di Monaco Zambarlal Bhujabal Ashish Jain Nitha C. Mulakkal Anthimi Palara Emma L. Powell Bonita Chung Cleidiane Zampronio Alexandra Jones Alexander Cameron Terje Johansen Ioannis P. Nezis Regulation of Expression of Autophagy Genes by Atg8a-Interacting Partners Sequoia, YL-1, and Sir2 in Drosophila Cell Reports acetylation autophagy LIR motif nucleus transcription LC3/Atg8 |
| title | Regulation of Expression of Autophagy Genes by Atg8a-Interacting Partners Sequoia, YL-1, and Sir2 in Drosophila |
| title_full | Regulation of Expression of Autophagy Genes by Atg8a-Interacting Partners Sequoia, YL-1, and Sir2 in Drosophila |
| title_fullStr | Regulation of Expression of Autophagy Genes by Atg8a-Interacting Partners Sequoia, YL-1, and Sir2 in Drosophila |
| title_full_unstemmed | Regulation of Expression of Autophagy Genes by Atg8a-Interacting Partners Sequoia, YL-1, and Sir2 in Drosophila |
| title_short | Regulation of Expression of Autophagy Genes by Atg8a-Interacting Partners Sequoia, YL-1, and Sir2 in Drosophila |
| title_sort | regulation of expression of autophagy genes by atg8a interacting partners sequoia yl 1 and sir2 in drosophila |
| topic | acetylation autophagy LIR motif nucleus transcription LC3/Atg8 |
| url | http://www.sciencedirect.com/science/article/pii/S2211124720306483 |
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