Study of the impact of organic minerals on spermatozoal gene expression in Osmanabadi bucks
Sperm transcripts aside from providing genetic material have an eminent role in post-fertilization events and embryonic development. The current study was carried out to study the gene expression in Osmanabadi buck (Capra hircus) supplemented with organic minerals. Organic minerals alone or in comb...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Indian Council of Agricultural Research
2024-04-01
|
| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/147248 |
| _version_ | 1827293852703129600 |
|---|---|
| author | S BACKIALAKSHMI A ARANGASAMY J SHARANYA NAIDU J GHOSH R BHATTA |
| author_facet | S BACKIALAKSHMI A ARANGASAMY J SHARANYA NAIDU J GHOSH R BHATTA |
| author_sort | S BACKIALAKSHMI |
| collection | DOAJ |
| description |
Sperm transcripts aside from providing genetic material have an eminent role in post-fertilization events and embryonic development. The current study was carried out to study the gene expression in Osmanabadi buck (Capra hircus) supplemented with organic minerals. Organic minerals alone or in combination with different concentrations were fed to the treatment groups (T2- Zn 20 mg, T3- Zn 40 mg, T4- Zn 60 mg, T5- Cu 12.5 mg, T6- Cu 25 mg, T7- Cu 37.5 mg, T8- Zn 20 mg + Cu 12.5 mg, T9- Zn 40 mg + Cu 25 mg, T10- Zn 60 mg + Cu 37.5 mg) along with the concentration mixture and roughages; whereas control (T1) group was fed concentrate mixture and roughages. The sperm samples were processed for gene expression studies of Ras Homolog Family Member A (RHOA), Mesoderm Specific Transcript (MEST), Nucleoside diphosphate-linked moiety X motif 6 (NUDT6), Protamine 1 (PRM1), Protamine 2 (PRM2) and Heat shock protein 90 alpha family class A member 1 (HSP90AA) using qRT-PCR and the protein-protein interaction analysis was carried out through String database version 11.0. Supplementation of trace minerals has enhanced the gene expression at a significant level (P<0.05) in the supplemented groups. The expression of MEST, RHOA, and PRM1 was higher in Cu 25-supplemented groups; whereas, NUDT6 in Zn 20, PRM2 and HSP90AA in Zn 40 showed higher expressions. Osmanabadi bucks supplemented with Cu 25 mg have shown promising results in the present and earlier studies so it can be concluded that the field-level application of Cu 25 mg would lead to promising results.
|
| first_indexed | 2024-04-24T13:46:03Z |
| format | Article |
| id | doaj.art-4c67d7c8b04a44d38b248d0a2da2cf54 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-24T13:46:03Z |
| publishDate | 2024-04-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-4c67d7c8b04a44d38b248d0a2da2cf542024-04-04T06:00:24ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272024-04-0194410.56093/ijans.v94i4.147248Study of the impact of organic minerals on spermatozoal gene expression in Osmanabadi bucksS BACKIALAKSHMI0A ARANGASAMY1J SHARANYA NAIDU2J GHOSH3R BHATTA4Jain Deemed to be University, Bengaluru, KarnatakaICAR-National Institute of Animal Nutrition and Physiology, Bengaluru, KarnatakaJain Deemed to be University, Bengaluru, KarnatakaICAR-National Institute of Animal Nutrition and Physiology, Bengaluru, KarnatakaDivision of Animal Science, Krishi Bhavan, New Delhi Sperm transcripts aside from providing genetic material have an eminent role in post-fertilization events and embryonic development. The current study was carried out to study the gene expression in Osmanabadi buck (Capra hircus) supplemented with organic minerals. Organic minerals alone or in combination with different concentrations were fed to the treatment groups (T2- Zn 20 mg, T3- Zn 40 mg, T4- Zn 60 mg, T5- Cu 12.5 mg, T6- Cu 25 mg, T7- Cu 37.5 mg, T8- Zn 20 mg + Cu 12.5 mg, T9- Zn 40 mg + Cu 25 mg, T10- Zn 60 mg + Cu 37.5 mg) along with the concentration mixture and roughages; whereas control (T1) group was fed concentrate mixture and roughages. The sperm samples were processed for gene expression studies of Ras Homolog Family Member A (RHOA), Mesoderm Specific Transcript (MEST), Nucleoside diphosphate-linked moiety X motif 6 (NUDT6), Protamine 1 (PRM1), Protamine 2 (PRM2) and Heat shock protein 90 alpha family class A member 1 (HSP90AA) using qRT-PCR and the protein-protein interaction analysis was carried out through String database version 11.0. Supplementation of trace minerals has enhanced the gene expression at a significant level (P<0.05) in the supplemented groups. The expression of MEST, RHOA, and PRM1 was higher in Cu 25-supplemented groups; whereas, NUDT6 in Zn 20, PRM2 and HSP90AA in Zn 40 showed higher expressions. Osmanabadi bucks supplemented with Cu 25 mg have shown promising results in the present and earlier studies so it can be concluded that the field-level application of Cu 25 mg would lead to promising results. https://epubs.icar.org.in/index.php/IJAnS/article/view/147248Bucks, Copper, Fertility, Gene expression, Organic minerals, Spermatozoa, Transcript, Zinc |
| spellingShingle | S BACKIALAKSHMI A ARANGASAMY J SHARANYA NAIDU J GHOSH R BHATTA Study of the impact of organic minerals on spermatozoal gene expression in Osmanabadi bucks Indian Journal of Animal Sciences Bucks, Copper, Fertility, Gene expression, Organic minerals, Spermatozoa, Transcript, Zinc |
| title | Study of the impact of organic minerals on spermatozoal gene expression in Osmanabadi bucks |
| title_full | Study of the impact of organic minerals on spermatozoal gene expression in Osmanabadi bucks |
| title_fullStr | Study of the impact of organic minerals on spermatozoal gene expression in Osmanabadi bucks |
| title_full_unstemmed | Study of the impact of organic minerals on spermatozoal gene expression in Osmanabadi bucks |
| title_short | Study of the impact of organic minerals on spermatozoal gene expression in Osmanabadi bucks |
| title_sort | study of the impact of organic minerals on spermatozoal gene expression in osmanabadi bucks |
| topic | Bucks, Copper, Fertility, Gene expression, Organic minerals, Spermatozoa, Transcript, Zinc |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/147248 |
| work_keys_str_mv | AT sbackialakshmi studyoftheimpactoforganicmineralsonspermatozoalgeneexpressioninosmanabadibucks AT aarangasamy studyoftheimpactoforganicmineralsonspermatozoalgeneexpressioninosmanabadibucks AT jsharanyanaidu studyoftheimpactoforganicmineralsonspermatozoalgeneexpressioninosmanabadibucks AT jghosh studyoftheimpactoforganicmineralsonspermatozoalgeneexpressioninosmanabadibucks AT rbhatta studyoftheimpactoforganicmineralsonspermatozoalgeneexpressioninosmanabadibucks |