Serological, Molecular Prevalence and Genotyping of <i>Coxiella burnetii</i> in Dairy Cattle Herds in Northeastern Algeria
In Algeria, data on the epidemiology of coxiellosis in cattle are still lacking. In this study, bulk tank milk (BTM) samples from 200 randomly selected dairy cattle herds from Setif province of Algeria were analyzed by an indirect enzyme-linked immunosorbent assay (ELISA) and polymerase chain reacti...
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| Format: | Article |
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MDPI AG
2022-01-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/9/2/40 |
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| author | Salah Eddine Menadi Valentina Chisu Cinzia Santucciu Marco Di Domenico Valentina Curini Giovanna Masala |
| author_facet | Salah Eddine Menadi Valentina Chisu Cinzia Santucciu Marco Di Domenico Valentina Curini Giovanna Masala |
| author_sort | Salah Eddine Menadi |
| collection | DOAJ |
| description | In Algeria, data on the epidemiology of coxiellosis in cattle are still lacking. In this study, bulk tank milk (BTM) samples from 200 randomly selected dairy cattle herds from Setif province of Algeria were analyzed by an indirect enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). Results highlighted that 37% (95% CI: 30.31–43.69%) and 9% (95% CI: 5.03–12.96%) of BTM samples contained <i>Coxiella burnetii</i> antibodies and DNA, respectively. Based on Cohen’s kappa coefficient, a very low agreement between the ELISA and PCR results was found (k = 0.0849) (95% CI: 0.00–0.189). For a second experiment, 186 whole blood samples of cows from farms with reproduction disorders were analyzed by molecular tools to detect C. burnetii. This study revealed an overall prevalence of 6.98% (95% CI: 3.32–10.65%). All positive samples determined by conventional PCR were analyzed by real-time quantitative PCR (qPCR). Eleven samples with cycle threshold (Ct) values lower than 35 were selected for genotyping by the multispacer sequence typing (MST) method. The MST12 genotype in BTM samples, the MST32 genotype and a new MST genotype (partial profile) in whole blood samples were identified. Obtained results have allowed us to better understand the epidemiology of bovine coxiellosis in the region of Setif. |
| first_indexed | 2024-03-09T20:53:25Z |
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| id | doaj.art-4c6d9bbf2c6741be9657bc638168f268 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-09T20:53:25Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
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| series | Veterinary Sciences |
| spelling | doaj.art-4c6d9bbf2c6741be9657bc638168f2682023-11-23T22:27:40ZengMDPI AGVeterinary Sciences2306-73812022-01-01924010.3390/vetsci9020040Serological, Molecular Prevalence and Genotyping of <i>Coxiella burnetii</i> in Dairy Cattle Herds in Northeastern AlgeriaSalah Eddine Menadi0Valentina Chisu1Cinzia Santucciu2Marco Di Domenico3Valentina Curini4Giovanna Masala5Research Laboratory Management of Local Animal Resources, High National Veterinary School “RABIE BOUCHAMA”, Street Issad Abbes, Oued Smar, Algiers 16270, AlgeriaZoonotic Pathology and OIE Reference Laboratory for Echinococcosis, National Reference Center for Echinococcosis (CeNRE), Istituto Zooprofilattico Sperimentale della Sardegna, 07100 Sassari, ItalyZoonotic Pathology and OIE Reference Laboratory for Echinococcosis, National Reference Center for Echinococcosis (CeNRE), Istituto Zooprofilattico Sperimentale della Sardegna, 07100 Sassari, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise G. Caporale, 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise G. Caporale, 64100 Teramo, ItalyZoonotic Pathology and OIE Reference Laboratory for Echinococcosis, National Reference Center for Echinococcosis (CeNRE), Istituto Zooprofilattico Sperimentale della Sardegna, 07100 Sassari, ItalyIn Algeria, data on the epidemiology of coxiellosis in cattle are still lacking. In this study, bulk tank milk (BTM) samples from 200 randomly selected dairy cattle herds from Setif province of Algeria were analyzed by an indirect enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). Results highlighted that 37% (95% CI: 30.31–43.69%) and 9% (95% CI: 5.03–12.96%) of BTM samples contained <i>Coxiella burnetii</i> antibodies and DNA, respectively. Based on Cohen’s kappa coefficient, a very low agreement between the ELISA and PCR results was found (k = 0.0849) (95% CI: 0.00–0.189). For a second experiment, 186 whole blood samples of cows from farms with reproduction disorders were analyzed by molecular tools to detect C. burnetii. This study revealed an overall prevalence of 6.98% (95% CI: 3.32–10.65%). All positive samples determined by conventional PCR were analyzed by real-time quantitative PCR (qPCR). Eleven samples with cycle threshold (Ct) values lower than 35 were selected for genotyping by the multispacer sequence typing (MST) method. The MST12 genotype in BTM samples, the MST32 genotype and a new MST genotype (partial profile) in whole blood samples were identified. Obtained results have allowed us to better understand the epidemiology of bovine coxiellosis in the region of Setif.https://www.mdpi.com/2306-7381/9/2/40<i>Coxiella burnetii</i>Q fevercoxiellosisbulk tank milk (BTM)ELISAPCR |
| spellingShingle | Salah Eddine Menadi Valentina Chisu Cinzia Santucciu Marco Di Domenico Valentina Curini Giovanna Masala Serological, Molecular Prevalence and Genotyping of <i>Coxiella burnetii</i> in Dairy Cattle Herds in Northeastern Algeria Veterinary Sciences <i>Coxiella burnetii</i> Q fever coxiellosis bulk tank milk (BTM) ELISA PCR |
| title | Serological, Molecular Prevalence and Genotyping of <i>Coxiella burnetii</i> in Dairy Cattle Herds in Northeastern Algeria |
| title_full | Serological, Molecular Prevalence and Genotyping of <i>Coxiella burnetii</i> in Dairy Cattle Herds in Northeastern Algeria |
| title_fullStr | Serological, Molecular Prevalence and Genotyping of <i>Coxiella burnetii</i> in Dairy Cattle Herds in Northeastern Algeria |
| title_full_unstemmed | Serological, Molecular Prevalence and Genotyping of <i>Coxiella burnetii</i> in Dairy Cattle Herds in Northeastern Algeria |
| title_short | Serological, Molecular Prevalence and Genotyping of <i>Coxiella burnetii</i> in Dairy Cattle Herds in Northeastern Algeria |
| title_sort | serological molecular prevalence and genotyping of i coxiella burnetii i in dairy cattle herds in northeastern algeria |
| topic | <i>Coxiella burnetii</i> Q fever coxiellosis bulk tank milk (BTM) ELISA PCR |
| url | https://www.mdpi.com/2306-7381/9/2/40 |
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