Microwave-Assisted Extraction Combined with In-Capillary [Fe(ferrozine)<sub>3</sub>]<sup>2+</sup>-CE-DAD to Screen Active Components with the Ability to Chelate Ferrous Ions from Flos Sophorae Immaturus (Flos Sophorae)
An efficient microwave-assisted extraction (MAE) combined with in-capillary [Fe(ferrozine)3]2+-capillary electrophoresis-Diode Array Detector (in-capillary [Fe(ferrozine)3]2+-CE-DAD) was developed to screen active components with the ability to chelate ferrous ions and determine the total antioxidan...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-08-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/24/17/3052 |
| _version_ | 1828889011599441920 |
|---|---|
| author | Tao Liu Shanshan Wang Huifen Ma Hua Jin Jin Li Xuejing Yang Xiumei Gao Yanxu Chang |
| author_facet | Tao Liu Shanshan Wang Huifen Ma Hua Jin Jin Li Xuejing Yang Xiumei Gao Yanxu Chang |
| author_sort | Tao Liu |
| collection | DOAJ |
| description | An efficient microwave-assisted extraction (MAE) combined with in-capillary [Fe(ferrozine)3]2+-capillary electrophoresis-Diode Array Detector (in-capillary [Fe(ferrozine)3]2+-CE-DAD) was developed to screen active components with the ability to chelate ferrous ions and determine the total antioxidant activity. The MAE conditions, including methanol concentration, extraction power, extraction time, and the ratio of material to liquid, were optimized by an L<sub>9</sub>(3<sup>4</sup>) orthogonal experiment. Background buffer, voltage, and cartridge temperature that affect the separation of six compounds were optimized. It was found that rutin and quercetin were the main components chelating ferrous ions in Flos Sophorae Immaturus (Flos Sophorae) by the in-capillary [Fe(ferrozine)<sub>3</sub>]<sup>2+</sup>-CE-DAD. The recoveries were ranged from 95.2% to 104%. It was concluded that the MAE combined with in-capillary [Fe(ferrozine)<sub>3</sub>]<sup>2+</sup>-CE-DAD method was a simple, reliable, and efficient tool for screening active components from the complex traditional Chinese medicine samples and evaluating their ability to chelate ferrous ions. |
| first_indexed | 2024-12-13T12:33:55Z |
| format | Article |
| id | doaj.art-4c6f5fc941454af289b5588cd7bb9b3f |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-13T12:33:55Z |
| publishDate | 2019-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-4c6f5fc941454af289b5588cd7bb9b3f2022-12-21T23:45:57ZengMDPI AGMolecules1420-30492019-08-012417305210.3390/molecules24173052molecules24173052Microwave-Assisted Extraction Combined with In-Capillary [Fe(ferrozine)<sub>3</sub>]<sup>2+</sup>-CE-DAD to Screen Active Components with the Ability to Chelate Ferrous Ions from Flos Sophorae Immaturus (Flos Sophorae)Tao Liu0Shanshan Wang1Huifen Ma2Hua Jin3Jin Li4Xuejing Yang5Xiumei Gao6Yanxu Chang7Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaTianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaTianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaTianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaTianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaTianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaTianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaTianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaAn efficient microwave-assisted extraction (MAE) combined with in-capillary [Fe(ferrozine)3]2+-capillary electrophoresis-Diode Array Detector (in-capillary [Fe(ferrozine)3]2+-CE-DAD) was developed to screen active components with the ability to chelate ferrous ions and determine the total antioxidant activity. The MAE conditions, including methanol concentration, extraction power, extraction time, and the ratio of material to liquid, were optimized by an L<sub>9</sub>(3<sup>4</sup>) orthogonal experiment. Background buffer, voltage, and cartridge temperature that affect the separation of six compounds were optimized. It was found that rutin and quercetin were the main components chelating ferrous ions in Flos Sophorae Immaturus (Flos Sophorae) by the in-capillary [Fe(ferrozine)<sub>3</sub>]<sup>2+</sup>-CE-DAD. The recoveries were ranged from 95.2% to 104%. It was concluded that the MAE combined with in-capillary [Fe(ferrozine)<sub>3</sub>]<sup>2+</sup>-CE-DAD method was a simple, reliable, and efficient tool for screening active components from the complex traditional Chinese medicine samples and evaluating their ability to chelate ferrous ions.https://www.mdpi.com/1420-3049/24/17/3052capillary electrophoresisMAE extractionability to chelate ferrous ionsFlos Sophorae ImmaturusFlos Sophorae |
| spellingShingle | Tao Liu Shanshan Wang Huifen Ma Hua Jin Jin Li Xuejing Yang Xiumei Gao Yanxu Chang Microwave-Assisted Extraction Combined with In-Capillary [Fe(ferrozine)<sub>3</sub>]<sup>2+</sup>-CE-DAD to Screen Active Components with the Ability to Chelate Ferrous Ions from Flos Sophorae Immaturus (Flos Sophorae) Molecules capillary electrophoresis MAE extraction ability to chelate ferrous ions Flos Sophorae Immaturus Flos Sophorae |
| title | Microwave-Assisted Extraction Combined with In-Capillary [Fe(ferrozine)<sub>3</sub>]<sup>2+</sup>-CE-DAD to Screen Active Components with the Ability to Chelate Ferrous Ions from Flos Sophorae Immaturus (Flos Sophorae) |
| title_full | Microwave-Assisted Extraction Combined with In-Capillary [Fe(ferrozine)<sub>3</sub>]<sup>2+</sup>-CE-DAD to Screen Active Components with the Ability to Chelate Ferrous Ions from Flos Sophorae Immaturus (Flos Sophorae) |
| title_fullStr | Microwave-Assisted Extraction Combined with In-Capillary [Fe(ferrozine)<sub>3</sub>]<sup>2+</sup>-CE-DAD to Screen Active Components with the Ability to Chelate Ferrous Ions from Flos Sophorae Immaturus (Flos Sophorae) |
| title_full_unstemmed | Microwave-Assisted Extraction Combined with In-Capillary [Fe(ferrozine)<sub>3</sub>]<sup>2+</sup>-CE-DAD to Screen Active Components with the Ability to Chelate Ferrous Ions from Flos Sophorae Immaturus (Flos Sophorae) |
| title_short | Microwave-Assisted Extraction Combined with In-Capillary [Fe(ferrozine)<sub>3</sub>]<sup>2+</sup>-CE-DAD to Screen Active Components with the Ability to Chelate Ferrous Ions from Flos Sophorae Immaturus (Flos Sophorae) |
| title_sort | microwave assisted extraction combined with in capillary fe ferrozine sub 3 sub sup 2 sup ce dad to screen active components with the ability to chelate ferrous ions from flos sophorae immaturus flos sophorae |
| topic | capillary electrophoresis MAE extraction ability to chelate ferrous ions Flos Sophorae Immaturus Flos Sophorae |
| url | https://www.mdpi.com/1420-3049/24/17/3052 |
| work_keys_str_mv | AT taoliu microwaveassistedextractioncombinedwithincapillaryfeferrozinesub3subsup2supcedadtoscreenactivecomponentswiththeabilitytochelateferrousionsfromflossophoraeimmaturusflossophorae AT shanshanwang microwaveassistedextractioncombinedwithincapillaryfeferrozinesub3subsup2supcedadtoscreenactivecomponentswiththeabilitytochelateferrousionsfromflossophoraeimmaturusflossophorae AT huifenma microwaveassistedextractioncombinedwithincapillaryfeferrozinesub3subsup2supcedadtoscreenactivecomponentswiththeabilitytochelateferrousionsfromflossophoraeimmaturusflossophorae AT huajin microwaveassistedextractioncombinedwithincapillaryfeferrozinesub3subsup2supcedadtoscreenactivecomponentswiththeabilitytochelateferrousionsfromflossophoraeimmaturusflossophorae AT jinli microwaveassistedextractioncombinedwithincapillaryfeferrozinesub3subsup2supcedadtoscreenactivecomponentswiththeabilitytochelateferrousionsfromflossophoraeimmaturusflossophorae AT xuejingyang microwaveassistedextractioncombinedwithincapillaryfeferrozinesub3subsup2supcedadtoscreenactivecomponentswiththeabilitytochelateferrousionsfromflossophoraeimmaturusflossophorae AT xiumeigao microwaveassistedextractioncombinedwithincapillaryfeferrozinesub3subsup2supcedadtoscreenactivecomponentswiththeabilitytochelateferrousionsfromflossophoraeimmaturusflossophorae AT yanxuchang microwaveassistedextractioncombinedwithincapillaryfeferrozinesub3subsup2supcedadtoscreenactivecomponentswiththeabilitytochelateferrousionsfromflossophoraeimmaturusflossophorae |