Silicon Actuates Poplar Calli Tolerance after Longer Exposure to Antimony
The presence of antimony (Sb) in high concentrations in the environment is recognized as an emerging problem worldwide. The toxicity of Sb in plant tissues is known; however, new methods of plant tolerance improvement must be addressed. Here, poplar callus (<i>Populus alba</i> L. var. py...
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MDPI AG
2023-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/3/689 |
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| author | Eva Labancová Zuzana Vivodová Kristína Šípošová Karin Kollárová |
| author_facet | Eva Labancová Zuzana Vivodová Kristína Šípošová Karin Kollárová |
| author_sort | Eva Labancová |
| collection | DOAJ |
| description | The presence of antimony (Sb) in high concentrations in the environment is recognized as an emerging problem worldwide. The toxicity of Sb in plant tissues is known; however, new methods of plant tolerance improvement must be addressed. Here, poplar callus (<i>Populus alba</i> L. var. pyramidallis) exposed to Sb(III) in 0.2 mM concentration and/or to silicon (Si) in 5 mM concentration was cultivated in vitro to determine the impact of Sb/Si interaction in the tissue. The Sb and Si uptake, growth, the activity of superoxide dismutase (SOD), catalase (CAT), guaiacol-peroxidase (G-POX), nutrient concentrations, and the concentrations of photosynthetic pigments were investigated. To elucidate the action of Si during the Sb-induced stress, the impact of short and long cultivations was determined. Silicon decreased the accumulation of Sb in the calli, regardless of the length of the cultivation (by approx. 34%). Antimony lowered the callus biomass (by approx. 37%) and decreased the concentrations of photosynthetic pigments (up to 78.5%) and nutrients in the tissue (up to 21.7%). Silicon supported the plant tolerance to Sb via the modification of antioxidant enzyme activity, which resulted in higher biomass production (increased by approx. 35%) and a higher uptake of nutrients from the media (increased by approx. 10%). Silicon aided the development of Sb-tolerance over the longer cultivation period. These results are key in understanding the action of Si-developed tolerance against metalloids. |
| first_indexed | 2024-03-11T09:29:18Z |
| format | Article |
| id | doaj.art-4c7168e4232e4f95b91a3258123ed72c |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T09:29:18Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-4c7168e4232e4f95b91a3258123ed72c2023-11-16T17:46:00ZengMDPI AGPlants2223-77472023-02-0112368910.3390/plants12030689Silicon Actuates Poplar Calli Tolerance after Longer Exposure to AntimonyEva Labancová0Zuzana Vivodová1Kristína Šípošová2Karin Kollárová3Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, SlovakiaInstitute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, SlovakiaInstitute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, SlovakiaInstitute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, SlovakiaThe presence of antimony (Sb) in high concentrations in the environment is recognized as an emerging problem worldwide. The toxicity of Sb in plant tissues is known; however, new methods of plant tolerance improvement must be addressed. Here, poplar callus (<i>Populus alba</i> L. var. pyramidallis) exposed to Sb(III) in 0.2 mM concentration and/or to silicon (Si) in 5 mM concentration was cultivated in vitro to determine the impact of Sb/Si interaction in the tissue. The Sb and Si uptake, growth, the activity of superoxide dismutase (SOD), catalase (CAT), guaiacol-peroxidase (G-POX), nutrient concentrations, and the concentrations of photosynthetic pigments were investigated. To elucidate the action of Si during the Sb-induced stress, the impact of short and long cultivations was determined. Silicon decreased the accumulation of Sb in the calli, regardless of the length of the cultivation (by approx. 34%). Antimony lowered the callus biomass (by approx. 37%) and decreased the concentrations of photosynthetic pigments (up to 78.5%) and nutrients in the tissue (up to 21.7%). Silicon supported the plant tolerance to Sb via the modification of antioxidant enzyme activity, which resulted in higher biomass production (increased by approx. 35%) and a higher uptake of nutrients from the media (increased by approx. 10%). Silicon aided the development of Sb-tolerance over the longer cultivation period. These results are key in understanding the action of Si-developed tolerance against metalloids.https://www.mdpi.com/2223-7747/12/3/689antimonyantioxidant enzymesnutrientsphotosynthetic pigments<i>Populus alba</i>silicon |
| spellingShingle | Eva Labancová Zuzana Vivodová Kristína Šípošová Karin Kollárová Silicon Actuates Poplar Calli Tolerance after Longer Exposure to Antimony Plants antimony antioxidant enzymes nutrients photosynthetic pigments <i>Populus alba</i> silicon |
| title | Silicon Actuates Poplar Calli Tolerance after Longer Exposure to Antimony |
| title_full | Silicon Actuates Poplar Calli Tolerance after Longer Exposure to Antimony |
| title_fullStr | Silicon Actuates Poplar Calli Tolerance after Longer Exposure to Antimony |
| title_full_unstemmed | Silicon Actuates Poplar Calli Tolerance after Longer Exposure to Antimony |
| title_short | Silicon Actuates Poplar Calli Tolerance after Longer Exposure to Antimony |
| title_sort | silicon actuates poplar calli tolerance after longer exposure to antimony |
| topic | antimony antioxidant enzymes nutrients photosynthetic pigments <i>Populus alba</i> silicon |
| url | https://www.mdpi.com/2223-7747/12/3/689 |
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