In vitro activity of rifampicin and verapamil combination in multidrug-resistant mycobacterium tuberculosis.
The aim of the present study was to evaluate the effect of the combination of rifampicin (RIF) and verapamil (VP) against the Mycobacterium tuberculosis H37Rv reference strain and six multidrug-resistant (MDR) M. tuberculosis clinical isolates by determining Time-Kill Curves and the ability to efflu...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4331551?pdf=render |
| _version_ | 1818386004604616704 |
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| author | Fernanda de Oliveira Demitto Renata Claro Ribeiro do Amaral Flaviane Granero Maltempe Vera Lúcia Dias Siqueira Regiane Bertin de Lima Scodro Mariana Aparecida Lopes Katiany R Caleffi-Ferracioli Pedro Henrique Canezin Rosilene Fressatti Cardoso |
| author_facet | Fernanda de Oliveira Demitto Renata Claro Ribeiro do Amaral Flaviane Granero Maltempe Vera Lúcia Dias Siqueira Regiane Bertin de Lima Scodro Mariana Aparecida Lopes Katiany R Caleffi-Ferracioli Pedro Henrique Canezin Rosilene Fressatti Cardoso |
| author_sort | Fernanda de Oliveira Demitto |
| collection | DOAJ |
| description | The aim of the present study was to evaluate the effect of the combination of rifampicin (RIF) and verapamil (VP) against the Mycobacterium tuberculosis H37Rv reference strain and six multidrug-resistant (MDR) M. tuberculosis clinical isolates by determining Time-Kill Curves and the ability to efflux drug by fluorometry. The RIF+VP combination showed synergism in one MDR clinical isolate. For the other five MDR clinical isolates, the drug combination showed no interaction. The MDR clinical isolate had lower ethidium bromide (EtBr) accumulation when exposed to the RIF+VP combination, compared with RIF and VP exposure alone. The other MDR clinical isolates showed no significant difference in EtBr accumulation. These results suggest greater efflux action in one of the MDR clinical isolates compared with the M. tuberculosis H37Rv reference strain. The other five MDR isolates may have additional mechanisms of drug resistance to RIF. The use of the RIF+VP combination made one MDR bacillus more susceptible to RIF probably by inhibiting efflux pumps, and this combination therapy, in some cases, may contribute to a reduction of resistance to RIF in M. tuberculosis. |
| first_indexed | 2024-12-14T03:47:09Z |
| format | Article |
| id | doaj.art-4c717d7bcc64444d8c1134e649b774b9 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T03:47:09Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4c717d7bcc64444d8c1134e649b774b92022-12-21T23:18:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01102e011654510.1371/journal.pone.0116545In vitro activity of rifampicin and verapamil combination in multidrug-resistant mycobacterium tuberculosis.Fernanda de Oliveira DemittoRenata Claro Ribeiro do AmaralFlaviane Granero MaltempeVera Lúcia Dias SiqueiraRegiane Bertin de Lima ScodroMariana Aparecida LopesKatiany R Caleffi-FerracioliPedro Henrique CanezinRosilene Fressatti CardosoThe aim of the present study was to evaluate the effect of the combination of rifampicin (RIF) and verapamil (VP) against the Mycobacterium tuberculosis H37Rv reference strain and six multidrug-resistant (MDR) M. tuberculosis clinical isolates by determining Time-Kill Curves and the ability to efflux drug by fluorometry. The RIF+VP combination showed synergism in one MDR clinical isolate. For the other five MDR clinical isolates, the drug combination showed no interaction. The MDR clinical isolate had lower ethidium bromide (EtBr) accumulation when exposed to the RIF+VP combination, compared with RIF and VP exposure alone. The other MDR clinical isolates showed no significant difference in EtBr accumulation. These results suggest greater efflux action in one of the MDR clinical isolates compared with the M. tuberculosis H37Rv reference strain. The other five MDR isolates may have additional mechanisms of drug resistance to RIF. The use of the RIF+VP combination made one MDR bacillus more susceptible to RIF probably by inhibiting efflux pumps, and this combination therapy, in some cases, may contribute to a reduction of resistance to RIF in M. tuberculosis.http://europepmc.org/articles/PMC4331551?pdf=render |
| spellingShingle | Fernanda de Oliveira Demitto Renata Claro Ribeiro do Amaral Flaviane Granero Maltempe Vera Lúcia Dias Siqueira Regiane Bertin de Lima Scodro Mariana Aparecida Lopes Katiany R Caleffi-Ferracioli Pedro Henrique Canezin Rosilene Fressatti Cardoso In vitro activity of rifampicin and verapamil combination in multidrug-resistant mycobacterium tuberculosis. PLoS ONE |
| title | In vitro activity of rifampicin and verapamil combination in multidrug-resistant mycobacterium tuberculosis. |
| title_full | In vitro activity of rifampicin and verapamil combination in multidrug-resistant mycobacterium tuberculosis. |
| title_fullStr | In vitro activity of rifampicin and verapamil combination in multidrug-resistant mycobacterium tuberculosis. |
| title_full_unstemmed | In vitro activity of rifampicin and verapamil combination in multidrug-resistant mycobacterium tuberculosis. |
| title_short | In vitro activity of rifampicin and verapamil combination in multidrug-resistant mycobacterium tuberculosis. |
| title_sort | in vitro activity of rifampicin and verapamil combination in multidrug resistant mycobacterium tuberculosis |
| url | http://europepmc.org/articles/PMC4331551?pdf=render |
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