Lipomodulatory and anti-oxidative stress effects of a polyherbal formulation based on garlic and avocado seed extracts on high fat high sucrose diet fed rats
Objective: To determine antioxidant potentials of Allium sativum and Persea americana seeds extracts and three formulation-based extracts in vitro, and to evaluate the effects of the best formulation on oxidative stress and dyslipidemia on rats fed with high fat and high sucrose diet (HFHSD). Method...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Metabolism Open |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589936822000330 |
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| author | Boris K.G. Azantsa Ntentie F. Raissa Mbong A. Mary-Ann Mafongang Amelie Kamtchoum Alexine Momo Cliffbrown Chimou N. Lauriane Fonkoua Martin Edoun E. Ferdinand Ngondi J. Laure Julius E. Oben |
| author_facet | Boris K.G. Azantsa Ntentie F. Raissa Mbong A. Mary-Ann Mafongang Amelie Kamtchoum Alexine Momo Cliffbrown Chimou N. Lauriane Fonkoua Martin Edoun E. Ferdinand Ngondi J. Laure Julius E. Oben |
| author_sort | Boris K.G. Azantsa |
| collection | DOAJ |
| description | Objective: To determine antioxidant potentials of Allium sativum and Persea americana seeds extracts and three formulation-based extracts in vitro, and to evaluate the effects of the best formulation on oxidative stress and dyslipidemia on rats fed with high fat and high sucrose diet (HFHSD). Methods: Aqueous extracts of Allium sativum, Persia. americana and three formulations were mixed at various portions (A. s/P. a; w/w): F (1:1), F (3: 1), and F(1:3). They were then tested for their antioxidant potentials in vitro using FRAP, DPPH and NO radicals to identify the best formulation. Four hundred (400) mg/kg b.w. of formulation F(1:1) were administered once daily for 21 days to rats previously fed with HFHSD for 8 weeks. Standard diet, vitamin E, and Atorvastatin were used as controls. After 21 days, body weight, blood glucose, lipid markers, activities of transaminases and markers of the antioxidant systems were assessed. Results: The Formulation F(1:1) showed the best in vitro activity with IC50 values of 6.5 and 2.23 mg/mL respectively for FRAP and DPPH- radical scavenging capacity. HFHSD caused a depletion of antioxidants associated with an increase of pro-oxidants and all the lipid markers except HDL-c Treatment with F(1:1) significantly increased TAC, SOD, and catalase activities, while MDA, protein carbonyls, and NO levels decreased (p < 0.05). Formulation F(1:1) decreased triglycerides (119.88 ± 4.25 mg/dL) and LDL-c (3.78 ± 0.66 mg/dL) levels and significantly increased the HDL-c level: (108.07 ± 6.29 mg/mL). Furthermore, Formulation F(1:1) significantly caused weight loss (2.31%), reduced blood glucose levels (27.38%) and ALT activity. Conclusion: The formulation F(1:1) could be a good candidate for the prevention and treatment of oxidative stress, dyslipidemia and features of metabolic syndrome. |
| first_indexed | 2024-04-11T21:12:50Z |
| format | Article |
| id | doaj.art-4c781574e7e54bc290f471abb3ce74a1 |
| institution | Directory Open Access Journal |
| issn | 2589-9368 |
| language | English |
| last_indexed | 2024-04-11T21:12:50Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Metabolism Open |
| spelling | doaj.art-4c781574e7e54bc290f471abb3ce74a12022-12-22T04:02:58ZengElsevierMetabolism Open2589-93682022-09-0115100195Lipomodulatory and anti-oxidative stress effects of a polyherbal formulation based on garlic and avocado seed extracts on high fat high sucrose diet fed ratsBoris K.G. Azantsa0Ntentie F. Raissa1Mbong A. Mary-Ann2Mafongang Amelie3Kamtchoum Alexine4Momo Cliffbrown5Chimou N. Lauriane6Fonkoua Martin7Edoun E. Ferdinand8Ngondi J. Laure9Julius E. Oben10Laboratory of Nutrition and Nutritional Biochemistry, Department of Biochemistry, Faculty of Sciences, University of Yaounde 1, PO Box: 812, Yaounde, Cameroon; Corresponding author.Laboratory of Nutrition and Nutritional Biochemistry, Department of Biochemistry, Faculty of Sciences, University of Yaounde 1, PO Box: 812, Yaounde, Cameroon; Department of Life and Earth Science, Higher Teachers' Training College, PO Box: 55, Maroua, CameroonLaboratory of Nutrition and Nutritional Biochemistry, Department of Biochemistry, Faculty of Sciences, University of Yaounde 1, PO Box: 812, Yaounde, CameroonLaboratory of Nutrition and Nutritional Biochemistry, Department of Biochemistry, Faculty of Sciences, University of Yaounde 1, PO Box: 812, Yaounde, CameroonLaboratory of Nutrition and Nutritional Biochemistry, Department of Biochemistry, Faculty of Sciences, University of Yaounde 1, PO Box: 812, Yaounde, CameroonLaboratory of Nutrition and Nutritional Biochemistry, Department of Biochemistry, Faculty of Sciences, University of Yaounde 1, PO Box: 812, Yaounde, CameroonLaboratory of Nutrition and Nutritional Biochemistry, Department of Biochemistry, Faculty of Sciences, University of Yaounde 1, PO Box: 812, Yaounde, CameroonLaboratory of Nutrition and Nutritional Biochemistry, Department of Biochemistry, Faculty of Sciences, University of Yaounde 1, PO Box: 812, Yaounde, CameroonLaboratory of Nutrition and Nutritional Biochemistry, Department of Biochemistry, Faculty of Sciences, University of Yaounde 1, PO Box: 812, Yaounde, Cameroon; Centre for Food and Nutrition Research, Institute of Medical Research and Medicinal Plant Studies, MINRESI, P.O Box 13033, Yaounde, CameroonLaboratory of Nutrition and Nutritional Biochemistry, Department of Biochemistry, Faculty of Sciences, University of Yaounde 1, PO Box: 812, Yaounde, CameroonLaboratory of Nutrition and Nutritional Biochemistry, Department of Biochemistry, Faculty of Sciences, University of Yaounde 1, PO Box: 812, Yaounde, CameroonObjective: To determine antioxidant potentials of Allium sativum and Persea americana seeds extracts and three formulation-based extracts in vitro, and to evaluate the effects of the best formulation on oxidative stress and dyslipidemia on rats fed with high fat and high sucrose diet (HFHSD). Methods: Aqueous extracts of Allium sativum, Persia. americana and three formulations were mixed at various portions (A. s/P. a; w/w): F (1:1), F (3: 1), and F(1:3). They were then tested for their antioxidant potentials in vitro using FRAP, DPPH and NO radicals to identify the best formulation. Four hundred (400) mg/kg b.w. of formulation F(1:1) were administered once daily for 21 days to rats previously fed with HFHSD for 8 weeks. Standard diet, vitamin E, and Atorvastatin were used as controls. After 21 days, body weight, blood glucose, lipid markers, activities of transaminases and markers of the antioxidant systems were assessed. Results: The Formulation F(1:1) showed the best in vitro activity with IC50 values of 6.5 and 2.23 mg/mL respectively for FRAP and DPPH- radical scavenging capacity. HFHSD caused a depletion of antioxidants associated with an increase of pro-oxidants and all the lipid markers except HDL-c Treatment with F(1:1) significantly increased TAC, SOD, and catalase activities, while MDA, protein carbonyls, and NO levels decreased (p < 0.05). Formulation F(1:1) decreased triglycerides (119.88 ± 4.25 mg/dL) and LDL-c (3.78 ± 0.66 mg/dL) levels and significantly increased the HDL-c level: (108.07 ± 6.29 mg/mL). Furthermore, Formulation F(1:1) significantly caused weight loss (2.31%), reduced blood glucose levels (27.38%) and ALT activity. Conclusion: The formulation F(1:1) could be a good candidate for the prevention and treatment of oxidative stress, dyslipidemia and features of metabolic syndrome.http://www.sciencedirect.com/science/article/pii/S2589936822000330Polyherbal formulationOxidative stressDyslipidemiaMetabolic syndromeTreatment |
| spellingShingle | Boris K.G. Azantsa Ntentie F. Raissa Mbong A. Mary-Ann Mafongang Amelie Kamtchoum Alexine Momo Cliffbrown Chimou N. Lauriane Fonkoua Martin Edoun E. Ferdinand Ngondi J. Laure Julius E. Oben Lipomodulatory and anti-oxidative stress effects of a polyherbal formulation based on garlic and avocado seed extracts on high fat high sucrose diet fed rats Metabolism Open Polyherbal formulation Oxidative stress Dyslipidemia Metabolic syndrome Treatment |
| title | Lipomodulatory and anti-oxidative stress effects of a polyherbal formulation based on garlic and avocado seed extracts on high fat high sucrose diet fed rats |
| title_full | Lipomodulatory and anti-oxidative stress effects of a polyherbal formulation based on garlic and avocado seed extracts on high fat high sucrose diet fed rats |
| title_fullStr | Lipomodulatory and anti-oxidative stress effects of a polyherbal formulation based on garlic and avocado seed extracts on high fat high sucrose diet fed rats |
| title_full_unstemmed | Lipomodulatory and anti-oxidative stress effects of a polyherbal formulation based on garlic and avocado seed extracts on high fat high sucrose diet fed rats |
| title_short | Lipomodulatory and anti-oxidative stress effects of a polyherbal formulation based on garlic and avocado seed extracts on high fat high sucrose diet fed rats |
| title_sort | lipomodulatory and anti oxidative stress effects of a polyherbal formulation based on garlic and avocado seed extracts on high fat high sucrose diet fed rats |
| topic | Polyherbal formulation Oxidative stress Dyslipidemia Metabolic syndrome Treatment |
| url | http://www.sciencedirect.com/science/article/pii/S2589936822000330 |
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