Genetic Alterations in Papillary Thyroid Carcinoma With Hashimoto’s Thyroiditis： ANK3, an Indolent Maintainer of Papillary Thyroid Carcinoma
Hashimoto’s thyroiditis (TH) is a risk factor for the occurrence of papillary thyroid carcinoma (PTC), which is considered to be the most common type of thyroid cancer. In recent years, the prevalence of PTC with TH has been increasing, but little is known about the genetic alteration in PTC with TH...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-05-01
|
| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2022.894786/full |
| _version_ | 1818206417434181632 |
|---|---|
| author | Chao Zeng Jiali Long Chunmiao Deng Linying Xie Hongmei Ma Yimin Guo Shuguang Liu Min Deng |
| author_facet | Chao Zeng Jiali Long Chunmiao Deng Linying Xie Hongmei Ma Yimin Guo Shuguang Liu Min Deng |
| author_sort | Chao Zeng |
| collection | DOAJ |
| description | Hashimoto’s thyroiditis (TH) is a risk factor for the occurrence of papillary thyroid carcinoma (PTC), which is considered to be the most common type of thyroid cancer. In recent years, the prevalence of PTC with TH has been increasing, but little is known about the genetic alteration in PTC with TH. This study analyzed the mutation spectrum and mutation signature of somatic single nucleotide variants (SNV) for 10 non-tumor and tumor pair tissues of PTC with TH using whole-exome sequencing. The ANK3 protein expression was evaluated by immunohistochemistry in PTC with TH and PTC samples. Moreover, the functional role of ANK3 in PTC cells was determined by CCK-8 proliferation assay, colony formation assays, cell cycle analysis, cell invasion and migration and in vivo study through overexpression assay. Our results showed three distinct mutational signatures and the C>T/G>A substitution was the most common type of SNV. Gene-set enrichment analysis showed that most of the significantly mutated genes were enriched in the regulation of actin cytoskeleton signaling. Moreover, NCOR2, BPTF, ANK3, and PCSK5 were identified as the significantly mutated genes in PTC with TH, most of which have not been previously characterized. Unexpectedly, it was found that ANK3 was overexpressed in cytoplasm close to the membrane of PTC cells with TH and in almost all PTC cases, suggesting its role as a diagnostic marker of PTC. Ectopic expression of ANK3 suppressed invasion and migration, increased apoptosis of B-CPAP and TPC-1 cells. Moreover, our findings revealed that enhanced ANK3 expression inhibits growth of PTC cells both in vitro and in vivo. Ectopic expression of ANK3 significantly enhanced E-cadherin protein expression and inhibited PTC progression, at least in part, by suppression of epithelial-mesenchymal transition (EMT). Our study shows that ANK3 exerts an anti-oncogenic role in the development of PTC and might be an indolent maintainer of PTC. |
| first_indexed | 2024-12-12T04:12:42Z |
| format | Article |
| id | doaj.art-4c7921b8a4ba42d4a4df6483fca6e8f7 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-12T04:12:42Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-4c7921b8a4ba42d4a4df6483fca6e8f72022-12-22T00:38:35ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2022-05-011210.3389/fonc.2022.894786894786Genetic Alterations in Papillary Thyroid Carcinoma With Hashimoto’s Thyroiditis： ANK3, an Indolent Maintainer of Papillary Thyroid CarcinomaChao Zeng0Jiali Long1Chunmiao Deng2Linying Xie3Hongmei Ma4Yimin Guo5Shuguang Liu6Min Deng7Department of Pathology, The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaDepartment of Pathology, The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaDepartment of Pathology, The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaDepartment of Pathology, The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaDepartment of Pathology, The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaDepartment of Pathology, The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaDepartment of Pathology, The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen, ChinaAffiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou, ChinaHashimoto’s thyroiditis (TH) is a risk factor for the occurrence of papillary thyroid carcinoma (PTC), which is considered to be the most common type of thyroid cancer. In recent years, the prevalence of PTC with TH has been increasing, but little is known about the genetic alteration in PTC with TH. This study analyzed the mutation spectrum and mutation signature of somatic single nucleotide variants (SNV) for 10 non-tumor and tumor pair tissues of PTC with TH using whole-exome sequencing. The ANK3 protein expression was evaluated by immunohistochemistry in PTC with TH and PTC samples. Moreover, the functional role of ANK3 in PTC cells was determined by CCK-8 proliferation assay, colony formation assays, cell cycle analysis, cell invasion and migration and in vivo study through overexpression assay. Our results showed three distinct mutational signatures and the C>T/G>A substitution was the most common type of SNV. Gene-set enrichment analysis showed that most of the significantly mutated genes were enriched in the regulation of actin cytoskeleton signaling. Moreover, NCOR2, BPTF, ANK3, and PCSK5 were identified as the significantly mutated genes in PTC with TH, most of which have not been previously characterized. Unexpectedly, it was found that ANK3 was overexpressed in cytoplasm close to the membrane of PTC cells with TH and in almost all PTC cases, suggesting its role as a diagnostic marker of PTC. Ectopic expression of ANK3 suppressed invasion and migration, increased apoptosis of B-CPAP and TPC-1 cells. Moreover, our findings revealed that enhanced ANK3 expression inhibits growth of PTC cells both in vitro and in vivo. Ectopic expression of ANK3 significantly enhanced E-cadherin protein expression and inhibited PTC progression, at least in part, by suppression of epithelial-mesenchymal transition (EMT). Our study shows that ANK3 exerts an anti-oncogenic role in the development of PTC and might be an indolent maintainer of PTC.https://www.frontiersin.org/articles/10.3389/fonc.2022.894786/fullpapillary thyroid carcinomaSNVANK3EMTE-cadherin |
| spellingShingle | Chao Zeng Jiali Long Chunmiao Deng Linying Xie Hongmei Ma Yimin Guo Shuguang Liu Min Deng Genetic Alterations in Papillary Thyroid Carcinoma With Hashimoto’s Thyroiditis： ANK3, an Indolent Maintainer of Papillary Thyroid Carcinoma Frontiers in Oncology papillary thyroid carcinoma SNV ANK3 EMT E-cadherin |
| title | Genetic Alterations in Papillary Thyroid Carcinoma With Hashimoto’s Thyroiditis： ANK3, an Indolent Maintainer of Papillary Thyroid Carcinoma |
| title_full | Genetic Alterations in Papillary Thyroid Carcinoma With Hashimoto’s Thyroiditis： ANK3, an Indolent Maintainer of Papillary Thyroid Carcinoma |
| title_fullStr | Genetic Alterations in Papillary Thyroid Carcinoma With Hashimoto’s Thyroiditis： ANK3, an Indolent Maintainer of Papillary Thyroid Carcinoma |
| title_full_unstemmed | Genetic Alterations in Papillary Thyroid Carcinoma With Hashimoto’s Thyroiditis： ANK3, an Indolent Maintainer of Papillary Thyroid Carcinoma |
| title_short | Genetic Alterations in Papillary Thyroid Carcinoma With Hashimoto’s Thyroiditis： ANK3, an Indolent Maintainer of Papillary Thyroid Carcinoma |
| title_sort | genetic alterations in papillary thyroid carcinoma with hashimoto s thyroiditis ank3 an indolent maintainer of papillary thyroid carcinoma |
| topic | papillary thyroid carcinoma SNV ANK3 EMT E-cadherin |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2022.894786/full |
| work_keys_str_mv | AT chaozeng geneticalterationsinpapillarythyroidcarcinomawithhashimotosthyroiditisank3anindolentmaintainerofpapillarythyroidcarcinoma AT jialilong geneticalterationsinpapillarythyroidcarcinomawithhashimotosthyroiditisank3anindolentmaintainerofpapillarythyroidcarcinoma AT chunmiaodeng geneticalterationsinpapillarythyroidcarcinomawithhashimotosthyroiditisank3anindolentmaintainerofpapillarythyroidcarcinoma AT linyingxie geneticalterationsinpapillarythyroidcarcinomawithhashimotosthyroiditisank3anindolentmaintainerofpapillarythyroidcarcinoma AT hongmeima geneticalterationsinpapillarythyroidcarcinomawithhashimotosthyroiditisank3anindolentmaintainerofpapillarythyroidcarcinoma AT yiminguo geneticalterationsinpapillarythyroidcarcinomawithhashimotosthyroiditisank3anindolentmaintainerofpapillarythyroidcarcinoma AT shuguangliu geneticalterationsinpapillarythyroidcarcinomawithhashimotosthyroiditisank3anindolentmaintainerofpapillarythyroidcarcinoma AT mindeng geneticalterationsinpapillarythyroidcarcinomawithhashimotosthyroiditisank3anindolentmaintainerofpapillarythyroidcarcinoma |