Magnolol potently suppressed lipopolysaccharide-induced iNOS and COX-2 expression via downregulating MAPK and NF-κB signaling pathways
Magnolol is a hydroxylated biphenyl compound from the bark of Magnolia officinalis that has been reported to have various biological properties including anti-inflammation. However, the molecular mechanism of anti-inflammation remains unclear although it has been suggested that magnolol inhibits NO...
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| Format: | Article |
| Language: | English |
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Elsevier
2011-07-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464611000491 |
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| author | Ching-Shu Lai You-Syuan Lai Daih-Huang Kuo Chih-Hsiung Wu Chi-Tang Ho Min-Hsiung Pan |
| author_facet | Ching-Shu Lai You-Syuan Lai Daih-Huang Kuo Chih-Hsiung Wu Chi-Tang Ho Min-Hsiung Pan |
| author_sort | Ching-Shu Lai |
| collection | DOAJ |
| description | Magnolol is a hydroxylated biphenyl compound from the bark of Magnolia officinalis that has been reported to have various biological properties including anti-inflammation. However, the molecular mechanism of anti-inflammation remains unclear although it has been suggested that magnolol inhibits NO production in murine macrophage. In this study, we investigated the inhibitory effects of magnolol on the induction of NO synthase (NOS) and COX-2 in RAW 264.7 cells induced by lipopolysaccharide (LPS). Co-treatment with magnolol significantly inhibited LPS-stimulated iNOS and COX-2 protein and gene expression. Western blot analysis and reporter assay showed that magnolol reduced translocation of the p50 and p65 subunit by reducing the degradation and phosphorylation of inhibitor κB (IκB), and subsequent transcriptional activity of NF-κB. We also found that magnolol blocked LPS-induced phosphorylation of extracellular signal-regulated kinase (ERK) 1/2, Jun N-terminal kinase (JNK) 1/2 and phosphatidylinositiol 3-kinase (PI3 K)/Akt signaling but no p38 mitogen-activated protein kinase (MAPK). These results suggest that magnolol inhibits iNOS and COX-2 protein and gene expression by blocking the activation of NF-κB through interference with activation of PI3K/Akt and MAPK signaling. These findings suggest that magnolol may have potential to be developed into an effective anti-inflammatory agent. |
| first_indexed | 2024-12-24T04:18:14Z |
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| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-24T04:18:14Z |
| publishDate | 2011-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-4c7af206480e411bae61ac40fb881ac52022-12-21T17:15:53ZengElsevierJournal of Functional Foods1756-46462011-07-0133198206Magnolol potently suppressed lipopolysaccharide-induced iNOS and COX-2 expression via downregulating MAPK and NF-κB signaling pathwaysChing-Shu Lai0You-Syuan Lai1Daih-Huang Kuo2Chih-Hsiung Wu3Chi-Tang Ho4Min-Hsiung Pan5Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung 811, TaiwanDepartment of Seafood Science, National Kaohsiung Marine University, Kaohsiung 811, TaiwanDepartment of Pharmacy and Graduate Institute of Pharmaceutical Technology, Tajen University, Pingtung 907, TaiwanCenter of Excellence for Cancer Research, Taipei Medical University, Taipei, TaiwanDepartment of Food Science, Rutgers University, New Brunswick, NJ 08901, USADepartment of Seafood Science, National Kaohsiung Marine University, Kaohsiung 811, Taiwan; Corresponding author: Address: Department of Seafood Science, National Kaohsiung Marine University, No. 142, Haijhuan Road, Nanzih District, Kaohsiung 81143, Taiwan. Tel.: +886 7 361 7141x3623; fax: +886 7 361 1261.Magnolol is a hydroxylated biphenyl compound from the bark of Magnolia officinalis that has been reported to have various biological properties including anti-inflammation. However, the molecular mechanism of anti-inflammation remains unclear although it has been suggested that magnolol inhibits NO production in murine macrophage. In this study, we investigated the inhibitory effects of magnolol on the induction of NO synthase (NOS) and COX-2 in RAW 264.7 cells induced by lipopolysaccharide (LPS). Co-treatment with magnolol significantly inhibited LPS-stimulated iNOS and COX-2 protein and gene expression. Western blot analysis and reporter assay showed that magnolol reduced translocation of the p50 and p65 subunit by reducing the degradation and phosphorylation of inhibitor κB (IκB), and subsequent transcriptional activity of NF-κB. We also found that magnolol blocked LPS-induced phosphorylation of extracellular signal-regulated kinase (ERK) 1/2, Jun N-terminal kinase (JNK) 1/2 and phosphatidylinositiol 3-kinase (PI3 K)/Akt signaling but no p38 mitogen-activated protein kinase (MAPK). These results suggest that magnolol inhibits iNOS and COX-2 protein and gene expression by blocking the activation of NF-κB through interference with activation of PI3K/Akt and MAPK signaling. These findings suggest that magnolol may have potential to be developed into an effective anti-inflammatory agent.http://www.sciencedirect.com/science/article/pii/S1756464611000491MagnololInducible NO synthase (iNOS)NF-κBRAW 264.7 monocyte/macrophagesLipopolysaccharide (LPS)Mitogen-activated protein kinase (MAPK) |
| spellingShingle | Ching-Shu Lai You-Syuan Lai Daih-Huang Kuo Chih-Hsiung Wu Chi-Tang Ho Min-Hsiung Pan Magnolol potently suppressed lipopolysaccharide-induced iNOS and COX-2 expression via downregulating MAPK and NF-κB signaling pathways Journal of Functional Foods Magnolol Inducible NO synthase (iNOS) NF-κB RAW 264.7 monocyte/macrophages Lipopolysaccharide (LPS) Mitogen-activated protein kinase (MAPK) |
| title | Magnolol potently suppressed lipopolysaccharide-induced iNOS and COX-2 expression via downregulating MAPK and NF-κB signaling pathways |
| title_full | Magnolol potently suppressed lipopolysaccharide-induced iNOS and COX-2 expression via downregulating MAPK and NF-κB signaling pathways |
| title_fullStr | Magnolol potently suppressed lipopolysaccharide-induced iNOS and COX-2 expression via downregulating MAPK and NF-κB signaling pathways |
| title_full_unstemmed | Magnolol potently suppressed lipopolysaccharide-induced iNOS and COX-2 expression via downregulating MAPK and NF-κB signaling pathways |
| title_short | Magnolol potently suppressed lipopolysaccharide-induced iNOS and COX-2 expression via downregulating MAPK and NF-κB signaling pathways |
| title_sort | magnolol potently suppressed lipopolysaccharide induced inos and cox 2 expression via downregulating mapk and nf κb signaling pathways |
| topic | Magnolol Inducible NO synthase (iNOS) NF-κB RAW 264.7 monocyte/macrophages Lipopolysaccharide (LPS) Mitogen-activated protein kinase (MAPK) |
| url | http://www.sciencedirect.com/science/article/pii/S1756464611000491 |
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