Lignan from <i>Alnus japonica</i> Inhibits Adipocyte Differentiation via Cell Cycle and FOXO1 Regulation
In the present study, we isolated a lignan ((−)-(2<i>R</i>,3<i>R</i>)-1,4-<i>O</i>-diferuloylsecoisolariciresinol, DFS) from <i>Alnus japonica</i> and evaluated its antiobesity potential in vitro. We also determined its mechanism of action in a mouse p...
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MDPI AG
2020-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/15/3346 |
| _version_ | 1797561503710183424 |
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| author | Hyejin Lee Ji Hye Jeong Jae-Ha Ryu |
| author_facet | Hyejin Lee Ji Hye Jeong Jae-Ha Ryu |
| author_sort | Hyejin Lee |
| collection | DOAJ |
| description | In the present study, we isolated a lignan ((−)-(2<i>R</i>,3<i>R</i>)-1,4-<i>O</i>-diferuloylsecoisolariciresinol, DFS) from <i>Alnus japonica</i> and evaluated its antiobesity potential in vitro. We also determined its mechanism of action in a mouse pre-adipocyte 3T3-L1 cell line. DFS dose- and day-dependently inhibited adipogenesis by downregulation of adipogenic factors and lipid metabolism-regulating factors during adipocyte differentiation. In particular, DFS suppressed cell cycle-regulating factors and induced G0/G1 cell cycle arrest, implying that it had an inhibitory effect on mitotic clonal expansion which occurred at an early stage of adipogenesis. DFS also suppressed adipogenesis through decreasing Akt phosphorylation and increasing the level of Forkhead box protein-O1 (FOXO1). These results suggest that DFS may be a pharmacological candidate for the development of antiobesity, therapeutic, and nutraceutical products. |
| first_indexed | 2024-03-10T18:15:50Z |
| format | Article |
| id | doaj.art-4c7bbd72725349c9b17a2f3f4ae033cd |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T18:15:50Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-4c7bbd72725349c9b17a2f3f4ae033cd2023-11-20T07:42:46ZengMDPI AGMolecules1420-30492020-07-012515334610.3390/molecules25153346Lignan from <i>Alnus japonica</i> Inhibits Adipocyte Differentiation via Cell Cycle and FOXO1 RegulationHyejin Lee0Ji Hye Jeong1Jae-Ha Ryu2Research Institute of Pharmaceutical Sciences and College of Pharmacy, Sookmyung Women’s University, Seoul 04310, KoreaResearch Institute of Pharmaceutical Sciences and College of Pharmacy, Sookmyung Women’s University, Seoul 04310, KoreaResearch Institute of Pharmaceutical Sciences and College of Pharmacy, Sookmyung Women’s University, Seoul 04310, KoreaIn the present study, we isolated a lignan ((−)-(2<i>R</i>,3<i>R</i>)-1,4-<i>O</i>-diferuloylsecoisolariciresinol, DFS) from <i>Alnus japonica</i> and evaluated its antiobesity potential in vitro. We also determined its mechanism of action in a mouse pre-adipocyte 3T3-L1 cell line. DFS dose- and day-dependently inhibited adipogenesis by downregulation of adipogenic factors and lipid metabolism-regulating factors during adipocyte differentiation. In particular, DFS suppressed cell cycle-regulating factors and induced G0/G1 cell cycle arrest, implying that it had an inhibitory effect on mitotic clonal expansion which occurred at an early stage of adipogenesis. DFS also suppressed adipogenesis through decreasing Akt phosphorylation and increasing the level of Forkhead box protein-O1 (FOXO1). These results suggest that DFS may be a pharmacological candidate for the development of antiobesity, therapeutic, and nutraceutical products.https://www.mdpi.com/1420-3049/25/15/3346<i>Alnus japonica</i>lignan3T3-L1adipogenesisFOXO1 |
| spellingShingle | Hyejin Lee Ji Hye Jeong Jae-Ha Ryu Lignan from <i>Alnus japonica</i> Inhibits Adipocyte Differentiation via Cell Cycle and FOXO1 Regulation Molecules <i>Alnus japonica</i> lignan 3T3-L1 adipogenesis FOXO1 |
| title | Lignan from <i>Alnus japonica</i> Inhibits Adipocyte Differentiation via Cell Cycle and FOXO1 Regulation |
| title_full | Lignan from <i>Alnus japonica</i> Inhibits Adipocyte Differentiation via Cell Cycle and FOXO1 Regulation |
| title_fullStr | Lignan from <i>Alnus japonica</i> Inhibits Adipocyte Differentiation via Cell Cycle and FOXO1 Regulation |
| title_full_unstemmed | Lignan from <i>Alnus japonica</i> Inhibits Adipocyte Differentiation via Cell Cycle and FOXO1 Regulation |
| title_short | Lignan from <i>Alnus japonica</i> Inhibits Adipocyte Differentiation via Cell Cycle and FOXO1 Regulation |
| title_sort | lignan from i alnus japonica i inhibits adipocyte differentiation via cell cycle and foxo1 regulation |
| topic | <i>Alnus japonica</i> lignan 3T3-L1 adipogenesis FOXO1 |
| url | https://www.mdpi.com/1420-3049/25/15/3346 |
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